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阿胶酶解物抗氧化肽的分离与质谱分析
李笑尘; 闫利华; 王智民; 张启伟; 高建萍; 陈两绵; 王锦玉; 仝燕; 张贵锋
2013
Source Publication中国中药杂志
Issue07Pages:1021-1025
Abstract采用阴离子交换纤维及Sephadex G-25柱色谱法对阿胶酶解物进行分离纯化,以DPPH,ABTS法对阿胶酶解物及其不同部位进行体外抗氧化能力测定,从阿胶酶解物中筛选出体外抗氧化活性最强的部位,命名为GFC-1。GFC-1质量浓度为2.0g·L-1时对DPPH清除率为47.95%,0.40g·L-1时对ABTS清除率为97.20%;利用LC-ESI-MS/MS结合TurboSE-QUEST检索软件及Swiss-Prot数据库从GFC-1中鉴定出9个小分子肽,并从中识别出高重复核心序列GPAGPP*GPP*(P*为羟脯氨酸)。
Keyword阿胶 抗氧化肽 Lc-esi-ms/ms 氨基酸序列
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/10106
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
李笑尘,闫利华,王智民,等. 阿胶酶解物抗氧化肽的分离与质谱分析[J]. 中国中药杂志,2013(07):1021-1025.
APA 李笑尘.,闫利华.,王智民.,张启伟.,高建萍.,...&张贵锋.(2013).阿胶酶解物抗氧化肽的分离与质谱分析.中国中药杂志(07),1021-1025.
MLA 李笑尘,et al."阿胶酶解物抗氧化肽的分离与质谱分析".中国中药杂志 .07(2013):1021-1025.
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