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亲水改性聚氨酯纳米纤维酶膜催化油水两相体系转糖苷反应
栾盼盼; 张羽飞; 张松平; 姜艳军; 李鹏举; 苏志国; 高静
2014
Source Publication过程工程学报
Issue03Pages:469-475
Abstract利用静电纺丝法制备含LiCl的聚氨酯(PU)纳米纤维,用牛血清白蛋白(BSA)对纤维亲水改性,于其上固定β-D-半乳糖苷酶,提高酶在油水两相介质中催化转糖苷反应的活力.结果表明,以PU浓度26%(ω)的电纺液制备的直径240~300 nm的PU纤维膜在LiCl辅助作用下吸附BSA高达222 mg/g,使纤维膜的水接触角由103.7o降至77.3o.改性PU膜固定化酶比活力为1.59 U/mg,而未改性PU膜仅为其79.2%.改性膜固定化酶55℃下的活力半衰期高达游离酶的14倍,在4℃下储存45 d活力仍保持80%,而游离酶活力仅剩余16.3%;改性膜固定化酶重复催化42次转糖苷反应活力仍能保持31%.
Keyword静电纺丝 聚氨酯纳米纤维 半乳糖苷酶 油水两相 转糖苷反应 亲水改性
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/10231
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
栾盼盼,张羽飞,张松平,等. 亲水改性聚氨酯纳米纤维酶膜催化油水两相体系转糖苷反应[J]. 过程工程学报,2014(03):469-475.
APA 栾盼盼.,张羽飞.,张松平.,姜艳军.,李鹏举.,...&高静.(2014).亲水改性聚氨酯纳米纤维酶膜催化油水两相体系转糖苷反应.过程工程学报(03),469-475.
MLA 栾盼盼,et al."亲水改性聚氨酯纳米纤维酶膜催化油水两相体系转糖苷反应".过程工程学报 .03(2014):469-475.
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