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8-(6-氨基己烷)-氨基-NADH的制备和表征
马洪静; 王平; 苏志国; 张松平
2009
Source Publication过程工程学报
Issue05Pages:975-980
Abstract采用1,6-己二胺对烟酰胺辅酶(NADH)的腺嘌呤C8位点进行定点修饰,通过DEAE-Sepharose层析分离得到产物8-(6-氨基己烷)-氨基-NADH.经优化制备过程和分离条件,其收率可达60%.在pH2.2~8.0内系统考察了修饰产物的pH稳定性,结果表明在不同种类的缓冲液中,修饰后辅酶的稳定性均有显著提高,在pH6.5的柠檬酸盐和pH8.0的磷酸盐缓冲液中,半衰期分别延长11和5倍.通过利用乳酸脱氢酶(LDH)和谷氨酸脱氢酶(GDH)测定修饰前后辅酶的活性,发现相同反应条件下修饰后辅酶的反应速率分别是天然NADH的3.0~3.8倍(LDH)和1.3~1.4倍(GDH).
Keyword8-(6-氨基己烷)-氨基-nadh 辅酶修饰 辅酶稳定性 辅酶活性
Indexed ByCSCD
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Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/10242
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
马洪静,王平,苏志国,等. 8-(6-氨基己烷)-氨基-NADH的制备和表征[J]. 过程工程学报,2009(05):975-980.
APA 马洪静,王平,苏志国,&张松平.(2009).8-(6-氨基己烷)-氨基-NADH的制备和表征.过程工程学报(05),975-980.
MLA 马洪静,et al."8-(6-氨基己烷)-氨基-NADH的制备和表征".过程工程学报 .05(2009):975-980.
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