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顺式环氧琥珀酸水解酶酶活的快速测定方法
彭亮亮; 王自强; 王云山; 张利平; 苏志国
2010
Source Publication中国农业科技导报
Issue01Pages:135-138
Abstract在对产顺式环氧琥珀酸水解酶(ESH)的细胞超声破碎的基础上,通过正交实验对细胞中ESH生物转化生产L(+)-酒石酸过程中的底物浓度、酶浓度、转化时间进行了分析,建立了一种快速测定ESH酶活的方法,即1 g湿细胞,30 mL 0.2 mol/L的顺式环氧琥珀酸二钠(ES),置于37℃保温转化10 m in后,利用HPLC测定酒石酸含量。此方法具有快速、准确和细胞用量少的特点,可用于菌种选育、发酵过程检测、发酵条件优化及工业生产。
Keyword顺式环氧琥珀酸水解酶 超声破碎 L-酒石酸 生物转化
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/10283
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
彭亮亮,王自强,王云山,等. 顺式环氧琥珀酸水解酶酶活的快速测定方法[J]. 中国农业科技导报,2010(01):135-138.
APA 彭亮亮,王自强,王云山,张利平,&苏志国.(2010).顺式环氧琥珀酸水解酶酶活的快速测定方法.中国农业科技导报(01),135-138.
MLA 彭亮亮,et al."顺式环氧琥珀酸水解酶酶活的快速测定方法".中国农业科技导报 .01(2010):135-138.
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