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海洋底泥环境DNA提取与纯化方法比较研究
王晓辉; 黄李淑馨; 白凤武; 杜昱光
2014
Source Publication大连理工大学学报
Issue03Pages:272-277
Abstract为了探索有效的海洋底泥宏基因组DNA提取与纯化方法,分别采用CTAB结合SDS法、CTAB结合玻璃珠法、SDS结合蛋白酶K法和SoilMasterTM试剂盒法提取海洋底泥宏基因组DNA,用柱式腐殖酸清除试剂盒法与电洗脱法纯化宏基因组DNA,并对宏基因组DNA的产量与纯度进行评价.结果表明:SDS结合蛋白酶K法提取率最高,DNA片段完整;SoilMasterTM试剂盒法与CTAB结合SDS法次之;CTAB结合玻璃珠法有严重降解;电洗脱法可以得到高纯度、大于42kb宏基因组DNA片段.因此,SDS结合蛋白酶K法和电洗脱法提取纯化海洋近海底泥宏基因组DNA优于其他方法.
Keyword宏基因组dna 提取 纯化 海洋底泥
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/10481
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
王晓辉,黄李淑馨,白凤武,等. 海洋底泥环境DNA提取与纯化方法比较研究[J]. 大连理工大学学报,2014(03):272-277.
APA 王晓辉,黄李淑馨,白凤武,&杜昱光.(2014).海洋底泥环境DNA提取与纯化方法比较研究.大连理工大学学报(03),272-277.
MLA 王晓辉,et al."海洋底泥环境DNA提取与纯化方法比较研究".大连理工大学学报 .03(2014):272-277.
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