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胰高血糖素样肽-1在乙醇中的聚乙二醇修饰
王友傲; 翟艳琴; 雷建都; 马光辉; 苏志国
2010
Source Publication过程工程学报
Issue03Pages:593-597
Abstract以乙醇为溶剂,单甲氧基聚乙二醇琥珀酰亚胺碳酸酯(mPEG-SC)为修饰剂,对胰高血糖素样肽-1(GLP-1)进行了修饰反应条件的优化,同时对单修饰产物Mono-PEG-GLP-1进行分离纯化,并考察了其体外稳定性和体内活性.得到的优化反应条件为:GLP-1浓度1mg/mL,mPEG-SC与GLP-1摩尔比1:1,37℃下反应24h,该产物最高转化率达77%.采用冷冻离心方式对Mono-PEG-GLP-1进行初步分离和浓缩,然后经反相液相色谱进一步高效纯化,纯度可达97%以上.体外实验表明,Mono-PEG-GLP-1在血清中具有更好的稳定性及更强的抗胰蛋白酶消化能力.体内动物实验表明,Mono-PEG-GLP-1具有更好的降血糖作用.
Keyword胰高血糖素样肽-1 单甲氧基聚乙二醇琥珀酰亚胺碳酸酯 聚乙二醇修饰 乙醇
Indexed ByCSCD
CSCD IDCSCD:3912443
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Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/10498
Collection研究所(批量导入)
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GB/T 7714
王友傲,翟艳琴,雷建都,等. 胰高血糖素样肽-1在乙醇中的聚乙二醇修饰[J]. 过程工程学报,2010(03):593-597.
APA 王友傲,翟艳琴,雷建都,马光辉,&苏志国.(2010).胰高血糖素样肽-1在乙醇中的聚乙二醇修饰.过程工程学报(03),593-597.
MLA 王友傲,et al."胰高血糖素样肽-1在乙醇中的聚乙二醇修饰".过程工程学报 .03(2010):593-597.
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