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凝胶过滤层析参数对EV71病毒粗提液分离效果的影响
杨永华; 邵强; 皮川真; 杨全中; 苏志国
2011
Source Publication东北农业大学学报
Issue09Pages:136-140
Abstract通过凝胶过滤层析达到分离纯化EV71肠道病毒粗提液,以提高EV71的纯度。选用不同的洗脱流速、不同样品上样量、不同的缓冲溶液的pH值和不同盐浓度对EV71肠道病毒粗提液进行连续洗脱。结果表明,凝胶过滤层析的洗脱流速、样品上样量、缓冲溶液的pH值和盐浓度对EV71病毒均能不同程度地影响EV71病毒粗提液的分离效果。在试验范围内,选择洗脱流速为60 cm·h-1、样品的上样量为5%CV、缓冲溶液的pH为7.5和盐浓度为0.1 mol·L-1时,对EV71病毒粗提液的分离效果比较理想。
Keyword凝胶过滤层析 分离 Ev71 手足口病
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/10654
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
杨永华,邵强,皮川真,等. 凝胶过滤层析参数对EV71病毒粗提液分离效果的影响[J]. 东北农业大学学报,2011(09):136-140.
APA 杨永华,邵强,皮川真,杨全中,&苏志国.(2011).凝胶过滤层析参数对EV71病毒粗提液分离效果的影响.东北农业大学学报(09),136-140.
MLA 杨永华,et al."凝胶过滤层析参数对EV71病毒粗提液分离效果的影响".东北农业大学学报 .09(2011):136-140.
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