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德氏假单胞R-8菌脱硫的“硫饥饿”诱导机理
余志坚; 李焕杰; 王海胜; 李玉光; 闫艳春; 李信
2009
Source Publication应用与环境生物学报
Issue02Pages:235-239
Abstract以专一性脱硫菌德氏假单胞菌(Pseudomonas delafieldii)R-8为出发菌株,构建了含有重组质粒pRT-D的重组菌株R-8-D.在不同硫酸盐浓度条件下,研究了R-8和R-8-D脱硫的"硫饥饿"诱导机理,结果表明,在充足的Na2SO4(>0.023mmolL-1)条件下,R-8菌首先利用硫酸盐生长,脱硫酶的合成受阻遏,DBT不被利用,而R-8菌处于"硫饥饿"状态(Na2SO4浓度≤0.023mmolL-1)时,诱导了脱硫酶的合成,能利用DBT生长;Na2SO4在临界浓度以上时,R-8-D菌阻遏了脱硫基因的报告基因lacZ的表达,低于临界浓度时不抑制lacZ表达.本实验结果从细胞和分子水平上证实了R-8菌脱硫属"硫饥饿"诱导类型,并首次确定了脱硫微生物"硫饥饿"诱导的硫酸盐临界浓度为0.023mmolL-1,为构建高活性的、不受硫酸盐抑制的脱硫工程菌提供了理论依据和技术支持.图10表1参14
Keyword德氏假单胞菌 硫酸盐 Dbt “硫饥饿”诱导 Ppr9tt 脱硫基因
Indexed ByCSCD
CSCD IDCSCD:3737334
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Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/10689
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
余志坚,李焕杰,王海胜,等. 德氏假单胞R-8菌脱硫的“硫饥饿”诱导机理[J]. 应用与环境生物学报,2009(02):235-239.
APA 余志坚,李焕杰,王海胜,李玉光,闫艳春,&李信.(2009).德氏假单胞R-8菌脱硫的“硫饥饿”诱导机理.应用与环境生物学报(02),235-239.
MLA 余志坚,et al."德氏假单胞R-8菌脱硫的“硫饥饿”诱导机理".应用与环境生物学报 .02(2009):235-239.
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