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大肠杆菌中透膜小肽抑制α-Synuclein聚集的研究
张亨; 徐锐; 王俊卿; 熊平; 汪浩勇
2010
Source Publication生物技术
Issue05Pages:26-29
Abstract目的:在大肠杆菌中研究透膜小肽抑制α-Synuclein(Α53T,S129Α,WT)的异常聚集。方法:基于α-Syn核心区的疏水区域设计了5种包含7个精氨酸的多聚精氨酸多肽膜透过传输系统的小肽R7P1~R7P5;构建融合蛋白α-Syn-GFP,其中α-Syn基因融合于GFP上游,透膜小肽以双顺反子方式与α-Syn-GFP共表达;以文献报道的可抑制α-Syn异常聚集的小肽ASI1D(MRRGGAVVTG(R)6)为对照,通过监测整体细胞荧光强度研究透膜小肽影响α-Syn(Α53T,S129Α,WT)的异常聚集的情况。结果:5种小肽能不同程度的抑制α-Syn(Α53T,S129Α,WT)的异常聚集;和对照Pc相比,R7P4抑制α-Syn(A53T)异常聚集的效果提高了55%,R7P3抑制α-Syn(S129A)异常聚集的效果提高了64%,R7P4抑制α-Syn(WT)异常聚集的效果提高了39%。结论:在大肠杆菌中,透膜小肽可以有效地抑制α-Syn(Α53T,S129Α,WT)的异常聚集。
KeywordΑ-synuclein 透膜小肽 聚集抑制 帕金森症
Indexed ByCSCD
CSCD IDCSCD:4068909
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Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/10725
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
张亨,徐锐,王俊卿,等. 大肠杆菌中透膜小肽抑制α-Synuclein聚集的研究[J]. 生物技术,2010(05):26-29.
APA 张亨,徐锐,王俊卿,熊平,&汪浩勇.(2010).大肠杆菌中透膜小肽抑制α-Synuclein聚集的研究.生物技术(05),26-29.
MLA 张亨,et al."大肠杆菌中透膜小肽抑制α-Synuclein聚集的研究".生物技术 .05(2010):26-29.
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