CAS OpenIR  > 研究所(批量导入)
胰高血糖素样肽-1的聚乙二醇定点修饰
张磊; 翟艳琴; 雷建都; 马光辉; 苏志国
2009
Source Publication过程工程学报
Issue06Pages:1169-1173
Abstract采用分子量为5kDa的单甲氧基聚乙二醇琥珀酰亚胺碳酸酯(mPEG-SC)修饰胰高血糖素样肽-1(GLP-1),建立了GLP-1的定点修饰反应及分离纯化工艺,考察了修饰反应各因素对单修饰产物得率及体外活性的影响,得到优化修饰条件为:pH7.5,50mmol/L Na2HPO4-NaH2PO4缓冲液,GLP-1浓度1mg/mL,PEG/GLP-1摩尔比2:1,4℃下反应1h.在此条件下,单修饰PEG-GLP-1得率达50%.反相高效液相色谱分离纯化所得单修饰产品纯度达98%,体外活性保留为未修饰GLP-1的86%,其在Sprague-Dawley远交群大鼠体内的循环半衰期为60min,比原肽提高了20倍.
Keyword胰高血糖素样肽-1 聚乙二醇 定点修饰
Indexed ByCSCD
CSCD IDCSCD:3763597
Citation statistics
Cited Times:2[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/10740
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
张磊,翟艳琴,雷建都,等. 胰高血糖素样肽-1的聚乙二醇定点修饰[J]. 过程工程学报,2009(06):1169-1173.
APA 张磊,翟艳琴,雷建都,马光辉,&苏志国.(2009).胰高血糖素样肽-1的聚乙二醇定点修饰.过程工程学报(06),1169-1173.
MLA 张磊,et al."胰高血糖素样肽-1的聚乙二醇定点修饰".过程工程学报 .06(2009):1169-1173.
Files in This Item:
File Name/Size DocType Version Access License
胰高血糖素样肽_1的聚乙二醇定点修饰_张(995KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[张磊]'s Articles
[翟艳琴]'s Articles
[雷建都]'s Articles
Baidu academic
Similar articles in Baidu academic
[张磊]'s Articles
[翟艳琴]'s Articles
[雷建都]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[张磊]'s Articles
[翟艳琴]'s Articles
[雷建都]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.