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无水体系中环氧氯丙烷活化琼脂糖凝胶制备高载量固定化金属亲和层析介质
张松平; 丁锐; 苏志国; 王平
2010
Source Publication过程工程学报
Issue05Pages:971-975
Abstract在以二甲基亚砜为溶剂的无水体系中,利用环氧氯丙烷对琼脂糖凝胶Sepharose6FastFlow进行活化,并偶联亚氨基二乙酸和Cu2+制备了固定化金属亲和层析介质.结果表明,该体系中环氧氯丙烷对琼脂糖凝胶的活化效率大幅度提高,在40%(φ)环氧氯丙烷、0.02g/mLNaOH及50℃、反应时间4h的优化条件下,环氧基活化密度最高达165μmol/mL,较目前报道的最高值提高50%以上.最终所制介质的Cu2+螯合密度为128.3μmol/mL,对BSA的平衡吸附容量达2.05mmol/L.以0.5mol/L咪唑为洗脱剂,被吸附的BSA洗脱率可达90%以上.
Keyword固定化金属亲和层析 琼脂糖凝胶 环氧氯丙烷 活化
Indexed ByCSCD
CSCD IDCSCD:4042951
Citation statistics
Cited Times:1[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/10757
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
张松平,丁锐,苏志国,等. 无水体系中环氧氯丙烷活化琼脂糖凝胶制备高载量固定化金属亲和层析介质[J]. 过程工程学报,2010(05):971-975.
APA 张松平,丁锐,苏志国,&王平.(2010).无水体系中环氧氯丙烷活化琼脂糖凝胶制备高载量固定化金属亲和层析介质.过程工程学报(05),971-975.
MLA 张松平,et al."无水体系中环氧氯丙烷活化琼脂糖凝胶制备高载量固定化金属亲和层析介质".过程工程学报 .05(2010):971-975.
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