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大孔吸附树脂纯化芦笋皂苷工艺优化
赵芳春; 赵兵; 黄云祥
2012
Source Publication食品工业科技
Issue18Pages:248-252
Abstract以芦笋下脚料提取物作为原料,优化了大孔吸附树脂纯化芦笋皂苷的工艺。通过静态法,对8种大孔吸附树脂纯化芦笋皂苷的效果进行比较,结果表明HPD-100树脂纯化芦笋皂苷效果最佳,并对HPD-100大孔树脂纯化芦笋皂苷工艺进行了优化。在上样质量浓度15mg/mL、溶液处理量150mL、1mL/min流速过柱;吸附后,先用50mL蒸馏水洗去杂质,再用200mL80%乙醇洗脱,总皂苷的含量由14.26%提高到35.49%(mean±SD)。将初次纯化产物按照上述工艺再纯化一次,总皂苷含量可高于50%,达到国家五类新药的含量要求。HPD-100可较好地吸附分离芦笋皂苷,纯化效率高,且其操作简单、安全、成本低廉,有较高的推广应用价值。
Keyword芦笋 皂苷 大孔吸附树脂 纯化 Hpd-100
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/10805
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
赵芳春,赵兵,黄云祥. 大孔吸附树脂纯化芦笋皂苷工艺优化[J]. 食品工业科技,2012(18):248-252.
APA 赵芳春,赵兵,&黄云祥.(2012).大孔吸附树脂纯化芦笋皂苷工艺优化.食品工业科技(18),248-252.
MLA 赵芳春,et al."大孔吸附树脂纯化芦笋皂苷工艺优化".食品工业科技 .18(2012):248-252.
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