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咪唑和吡啶离子液体对青霉素酰化酶催化性能的影响
周华从; 李伟; 高红帅; 贾连伟; 杨良嵘; 徐芃; 刘会洲
2010
Source Publication过程工程学报
Issue06Pages:1181-1186
Abstract采用两步法合成并表征了阳离子不同的咪唑型和吡啶型离子液体[C4mim]BF4,[C7mim]BF4,[C4pyr]BF4和[C7pyr]BF4,并将其应用于固定化青霉素G酰化酶(ImPGA)催化分解青霉素G钾盐的反应,考察了阳离子对酶活性、稳定性、动力学的影响,及ImPGA在不同离子液体中酶活性受底物(青霉素G钾盐)抑制情况.结果表明,ImPGA在[C7pyr]BF4,[C4pyr]BF4和[C7mim]BF4中活性依次降低,在亲水性较强的[C4mim]BF4中易失去活性,其在3种离子液体中37℃下保存30min活性没有降低,米氏常数Km依次为752.15,160.57和62.74mmol/L,最大反应速率Vmax依次为0.204,0.042和0.024mmol/(g·min);底物浓度为26.85~295.32mmol/L时酶活性不受底物抑制.
Keyword青霉素酰化酶 离子液体 活性 稳定性 动力学
Indexed ByCSCD
CSCD IDCSCD:4101372
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Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/10848
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
周华从,李伟,高红帅,等. 咪唑和吡啶离子液体对青霉素酰化酶催化性能的影响[J]. 过程工程学报,2010(06):1181-1186.
APA 周华从.,李伟.,高红帅.,贾连伟.,杨良嵘.,...&刘会洲.(2010).咪唑和吡啶离子液体对青霉素酰化酶催化性能的影响.过程工程学报(06),1181-1186.
MLA 周华从,et al."咪唑和吡啶离子液体对青霉素酰化酶催化性能的影响".过程工程学报 .06(2010):1181-1186.
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