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| Enhanced Humoral and Cell-Mediated Immune Responses Generated by Cationic Polymer-Coated PLA Microspheres with Adsorbed HBsAg | |
| Alternative Title | Mol. Pharm. |
| Chen, Xiaoming1,2; Liu, Yuying3; Wang, Lianyan1; Liu, Yuan1,2; Zhang, Weifeng1,2; Fan, Bei4; Ma, Xiaowei4; Yuan, Qipeng3; Ma, Guanghui1; Su, Zhiguo1 | |
| 2014-06-01 | |
| Source Publication | MOLECULAR PHARMACEUTICS
 |
| ISSN | 1543-8384 |
| Volume | 11Issue:6Pages:1772-1784 |
| Abstract | Surface-engineered particulate delivery systems for vaccine administration have been widely investigated in experimental and clinical studies. However, little is known about charge-coated microspheres as potential recombinant subunit protein antigen delivery systems in terms of adsorption and related immune responses. In the present study, cationic polymers, including chitosan (CS), chitosan chloride (CSC), and polyethylenimine (PEI), were used to coat PIA microspheres to build positively charged surfaces. Antigen adsorption capacity was enhanced with increased surface charge of coated microspheres. In macrophages, HBsAg adsorbed on the surface of cationic microspheres specifically enhanced antigen uptake and augmented CD86, MHC I, and MHC II expression and IL-1 beta, IL-6, TNF-alpha, and IL-12 release. Antigens were more likely to localize independent of lysosomes after phagocytosis in antigen-attached cationic microsphere formulations. After intraperitoneal immunization, cationic microsphere-based vaccine formulations generated a rapid and efficient humoral immune response and cytokine release as compared with aluminum-adsorbed vaccine and free antigens in vivo. Moreover, microspheres coated with cationic polymers with relatively high positive charges and higher antigen adsorption exhibited strong stimulation of the Th1 response. In conclusion, PLA microspheres coated with cationic polymers may be a potential recombinant antigen delivery system to induce strong cell and humoral immune responses.; Surface-engineered particulate delivery systems for vaccine administration have been widely investigated in experimental and clinical studies. However, little is known about charge-coated microspheres as potential recombinant subunit protein antigen delivery systems in terms of adsorption and related immune responses. In the present study, cationic polymers, including chitosan (CS), chitosan chloride (CSC), and polyethylenimine (PEI), were used to coat PIA microspheres to build positively charged surfaces. Antigen adsorption capacity was enhanced with increased surface charge of coated microspheres. In macrophages, HBsAg adsorbed on the surface of cationic microspheres specifically enhanced antigen uptake and augmented CD86, MHC I, and MHC II expression and IL-1 beta, IL-6, TNF-alpha, and IL-12 release. Antigens were more likely to localize independent of lysosomes after phagocytosis in antigen-attached cationic microsphere formulations. After intraperitoneal immunization, cationic microsphere-based vaccine formulations generated a rapid and efficient humoral immune response and cytokine release as compared with aluminum-adsorbed vaccine and free antigens in vivo. Moreover, microspheres coated with cationic polymers with relatively high positive charges and higher antigen adsorption exhibited strong stimulation of the Th1 response. In conclusion, PLA microspheres coated with cationic polymers may be a potential recombinant antigen delivery system to induce strong cell and humoral immune responses. |
| Keyword | Pla Microsphere Cationic Polymer Hbsag Adsorption Th1 |
| Subtype | Article |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1021/mp400597z |
| URL | 查看原文 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | PLASMACYTOID DENDRITIC CELLS ; B-VIRUS INFECTION ; VACCINE ADJUVANT ; EXOGENOUS ANTIGENS ; ALUMINUM ADJUVANTS ; CROSS-PRESENTATION ; DNA VACCINES ; HIV-1 P24 ; T-CELLS ; DELIVERY |
| WOS Research Area | Research & Experimental Medicine ; Pharmacology & Pharmacy |
| WOS Subject | Medicine, Research & Experimental ; Pharmacology & Pharmacy |
| WOS ID | WOS:000336941900005 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Version | 出版稿 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/10889 |
| Collection | 研究所（批量导入） |
| Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Graduated Univ, Beijing 100049, Peoples R China 3.Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing 100029, Peoples R China 4.Hualan Biol Engn Inc, Xinxiang 453003, Henan, Peoples R China |
| Recommended Citation GB/T 7714 | Chen, Xiaoming,Liu, Yuying,Wang, Lianyan,et al. Enhanced Humoral and Cell-Mediated Immune Responses Generated by Cationic Polymer-Coated PLA Microspheres with Adsorbed HBsAg[J]. MOLECULAR PHARMACEUTICS,2014,11(6):1772-1784. |
| APA | Chen, Xiaoming.,Liu, Yuying.,Wang, Lianyan.,Liu, Yuan.,Zhang, Weifeng.,...&Su, Zhiguo.(2014).Enhanced Humoral and Cell-Mediated Immune Responses Generated by Cationic Polymer-Coated PLA Microspheres with Adsorbed HBsAg.MOLECULAR PHARMACEUTICS,11(6),1772-1784. |
| MLA | Chen, Xiaoming,et al."Enhanced Humoral and Cell-Mediated Immune Responses Generated by Cationic Polymer-Coated PLA Microspheres with Adsorbed HBsAg".MOLECULAR PHARMACEUTICS 11.6(2014):1772-1784. |
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| Enhanced Humoral and（7111KB） | 限制开放 | CC BY-NC-SA | Application Full Text | |||
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