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Enhanced Humoral and Cell-Mediated Immune Responses Generated by Cationic Polymer-Coated PLA Microspheres with Adsorbed HBsAg
Alternative TitleMol. Pharm.
Chen, Xiaoming1,2; Liu, Yuying3; Wang, Lianyan1; Liu, Yuan1,2; Zhang, Weifeng1,2; Fan, Bei4; Ma, Xiaowei4; Yuan, Qipeng3; Ma, Guanghui1; Su, Zhiguo1
2014-06-01
Source PublicationMOLECULAR PHARMACEUTICS
ISSN1543-8384
Volume11Issue:6Pages:1772-1784
AbstractSurface-engineered particulate delivery systems for vaccine administration have been widely investigated in experimental and clinical studies. However, little is known about charge-coated microspheres as potential recombinant subunit protein antigen delivery systems in terms of adsorption and related immune responses. In the present study, cationic polymers, including chitosan (CS), chitosan chloride (CSC), and polyethylenimine (PEI), were used to coat PIA microspheres to build positively charged surfaces. Antigen adsorption capacity was enhanced with increased surface charge of coated microspheres. In macrophages, HBsAg adsorbed on the surface of cationic microspheres specifically enhanced antigen uptake and augmented CD86, MHC I, and MHC II expression and IL-1 beta, IL-6, TNF-alpha, and IL-12 release. Antigens were more likely to localize independent of lysosomes after phagocytosis in antigen-attached cationic microsphere formulations. After intraperitoneal immunization, cationic microsphere-based vaccine formulations generated a rapid and efficient humoral immune response and cytokine release as compared with aluminum-adsorbed vaccine and free antigens in vivo. Moreover, microspheres coated with cationic polymers with relatively high positive charges and higher antigen adsorption exhibited strong stimulation of the Th1 response. In conclusion, PLA microspheres coated with cationic polymers may be a potential recombinant antigen delivery system to induce strong cell and humoral immune responses.; Surface-engineered particulate delivery systems for vaccine administration have been widely investigated in experimental and clinical studies. However, little is known about charge-coated microspheres as potential recombinant subunit protein antigen delivery systems in terms of adsorption and related immune responses. In the present study, cationic polymers, including chitosan (CS), chitosan chloride (CSC), and polyethylenimine (PEI), were used to coat PIA microspheres to build positively charged surfaces. Antigen adsorption capacity was enhanced with increased surface charge of coated microspheres. In macrophages, HBsAg adsorbed on the surface of cationic microspheres specifically enhanced antigen uptake and augmented CD86, MHC I, and MHC II expression and IL-1 beta, IL-6, TNF-alpha, and IL-12 release. Antigens were more likely to localize independent of lysosomes after phagocytosis in antigen-attached cationic microsphere formulations. After intraperitoneal immunization, cationic microsphere-based vaccine formulations generated a rapid and efficient humoral immune response and cytokine release as compared with aluminum-adsorbed vaccine and free antigens in vivo. Moreover, microspheres coated with cationic polymers with relatively high positive charges and higher antigen adsorption exhibited strong stimulation of the Th1 response. In conclusion, PLA microspheres coated with cationic polymers may be a potential recombinant antigen delivery system to induce strong cell and humoral immune responses.
KeywordPla Microsphere Cationic Polymer Hbsag Adsorption Th1
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1021/mp400597z
URL查看原文
Indexed BySCI
Language英语
WOS KeywordPLASMACYTOID DENDRITIC CELLS ; B-VIRUS INFECTION ; VACCINE ADJUVANT ; EXOGENOUS ANTIGENS ; ALUMINUM ADJUVANTS ; CROSS-PRESENTATION ; DNA VACCINES ; HIV-1 P24 ; T-CELLS ; DELIVERY
WOS Research AreaResearch & Experimental Medicine ; Pharmacology & Pharmacy
WOS SubjectMedicine, Research & Experimental ; Pharmacology & Pharmacy
WOS IDWOS:000336941900005
Citation statistics
Cited Times:42[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/10889
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Graduated Univ, Beijing 100049, Peoples R China
3.Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing 100029, Peoples R China
4.Hualan Biol Engn Inc, Xinxiang 453003, Henan, Peoples R China
Recommended Citation
GB/T 7714
Chen, Xiaoming,Liu, Yuying,Wang, Lianyan,et al. Enhanced Humoral and Cell-Mediated Immune Responses Generated by Cationic Polymer-Coated PLA Microspheres with Adsorbed HBsAg[J]. MOLECULAR PHARMACEUTICS,2014,11(6):1772-1784.
APA Chen, Xiaoming.,Liu, Yuying.,Wang, Lianyan.,Liu, Yuan.,Zhang, Weifeng.,...&Su, Zhiguo.(2014).Enhanced Humoral and Cell-Mediated Immune Responses Generated by Cationic Polymer-Coated PLA Microspheres with Adsorbed HBsAg.MOLECULAR PHARMACEUTICS,11(6),1772-1784.
MLA Chen, Xiaoming,et al."Enhanced Humoral and Cell-Mediated Immune Responses Generated by Cationic Polymer-Coated PLA Microspheres with Adsorbed HBsAg".MOLECULAR PHARMACEUTICS 11.6(2014):1772-1784.
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