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Inhibition of Cellulase, b-Glucosidase, and Xylanase Activities and Enzymatic Hydrolysis of Dilute Acid Pretreated Wheat Straw by Acetone-Butanol-Ethanol Fermentation Products
Alternative TitleEnviron. Prog. Sustain. Energy
Qi, Benkun; Chen, Xiangrong; Yi, Su; Wan, Yinhua
2014-07-01
Source PublicationENVIRONMENTAL PROGRESS & SUSTAINABLE ENERGY
ISSN1944-7442
Volume33Issue:2Pages:497-503
AbstractSimultaneous saccharification and fermentation has been regarded as a promising process for bioconversion of lignocellulosic biomass to acetone-butanol-ethanol (ABE); however, there exits potential inhibition of cellulase, -glucosidase, and xylanase by the fermentation product(s) due to the combination of enzymatic hydrolysis and microbial fermentation in one vessel. The effects of ABE fermentation products including acetone, butanol, ethanol, acetic acid, and butyric acid, individually and in a combination, on the cellulase, -glucosidase, and xylanase activities and enzymatic saccharification of dilute acid pretreated wheat straw (WS) were studied. The experimental results showed that butanol and mixture of inhibitor compounds strongly depressed cellulolytic and hemicellulolytic activities, and no synergistic inhibiting effect between inhibitors on enzyme activities was observed. Saccharification of pretreated WS was mainly affected by butanol and temperature. Moreover, preincubation of enzyme solutions with inhibitors mixture for 72 h decreased cellulase and xylanase activities by 15-25% at the three temperatures examined (30, 37, and 50 degrees C), and a significant decrease (64%) in -glucosidase activity was found at 50 degrees C. (c) 2013 American Institute of Chemical Engineers Environ Prog, 33: 497-503, 2014; Simultaneous saccharification and fermentation has been regarded as a promising process for bioconversion of lignocellulosic biomass to acetone-butanol-ethanol (ABE); however, there exits potential inhibition of cellulase, -glucosidase, and xylanase by the fermentation product(s) due to the combination of enzymatic hydrolysis and microbial fermentation in one vessel. The effects of ABE fermentation products including acetone, butanol, ethanol, acetic acid, and butyric acid, individually and in a combination, on the cellulase, -glucosidase, and xylanase activities and enzymatic saccharification of dilute acid pretreated wheat straw (WS) were studied. The experimental results showed that butanol and mixture of inhibitor compounds strongly depressed cellulolytic and hemicellulolytic activities, and no synergistic inhibiting effect between inhibitors on enzyme activities was observed. Saccharification of pretreated WS was mainly affected by butanol and temperature. Moreover, preincubation of enzyme solutions with inhibitors mixture for 72 h decreased cellulase and xylanase activities by 15-25% at the three temperatures examined (30, 37, and 50 degrees C), and a significant decrease (64%) in -glucosidase activity was found at 50 degrees C. (c) 2013 American Institute of Chemical Engineers Environ Prog, 33: 497-503, 2014
KeywordAcetone-butanol-ethanol Cellulase Enzymatic Hydrolysis -glucosidase Xylanase
SubtypeArticle
WOS HeadingsScience & Technology ; Technology ; Life Sciences & Biomedicine
DOI10.1002/ep.11789
URL查看原文
Indexed BySCI
Language英语
WOS KeywordSIMULTANEOUS SACCHARIFICATION ; CLOSTRIDIUM-BEIJERINCKII ; HARDWOOD ; BIOMASS ; BIOFUEL
WOS Research AreaEngineering ; Environmental Sciences & Ecology
WOS SubjectEngineering, Environmental ; Engineering, Chemical ; Engineering, Industrial ; Environmental Sciences
WOS IDWOS:000334298800021
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/10949
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Qi, Benkun,Chen, Xiangrong,Yi, Su,et al. Inhibition of Cellulase, b-Glucosidase, and Xylanase Activities and Enzymatic Hydrolysis of Dilute Acid Pretreated Wheat Straw by Acetone-Butanol-Ethanol Fermentation Products[J]. ENVIRONMENTAL PROGRESS & SUSTAINABLE ENERGY,2014,33(2):497-503.
APA Qi, Benkun,Chen, Xiangrong,Yi, Su,&Wan, Yinhua.(2014).Inhibition of Cellulase, b-Glucosidase, and Xylanase Activities and Enzymatic Hydrolysis of Dilute Acid Pretreated Wheat Straw by Acetone-Butanol-Ethanol Fermentation Products.ENVIRONMENTAL PROGRESS & SUSTAINABLE ENERGY,33(2),497-503.
MLA Qi, Benkun,et al."Inhibition of Cellulase, b-Glucosidase, and Xylanase Activities and Enzymatic Hydrolysis of Dilute Acid Pretreated Wheat Straw by Acetone-Butanol-Ethanol Fermentation Products".ENVIRONMENTAL PROGRESS & SUSTAINABLE ENERGY 33.2(2014):497-503.
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