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Natural laccase mediators separated from water-washed solution of steam exploded corn straw by nanofiltration and organic solvent fractionation
Alternative TitleBioresour. Technol.
Qiu, Weihua; Zhang, Wenyan; Chen, Hongzhang
2014-03-01
Source PublicationBIORESOURCE TECHNOLOGY
ISSN0960-8524
Volume156Issue:1Pages:368-371
AbstractArtificially synthetic mediators of laccase had the limitation of high cost and possible toxicity. The separation of natural laccase mediators from water-washed solution (WWS) of steam exploded corn straw (SECS) was studied using nano-filtration and successive organic solvents extraction. Results indicated that the UV absorption intensity of nano-filtrated WWS was significantly enhanced. The UV absorption intensity of each extractive from WWS could be ranked as ether extractive (EE) > ethyl acetate extractive (EAE) > chloroform extractive (CE). Decoloration of crystal violet catalyzed by laccase/EE was higher than that by laccase/ABTS, which was 66.95% and 61.9% at 8 h, respectively. All the decoloration rates of malachite green at 60 min using EE, EAE and ABTS as mediator were both more than 80%. This research would benefit for broaden the source of laccase mediator and reduce the using cost of laccase/mediator system. (C) 2014 Elsevier Ltd. All rights reserved.; Artificially synthetic mediators of laccase had the limitation of high cost and possible toxicity. The separation of natural laccase mediators from water-washed solution (WWS) of steam exploded corn straw (SECS) was studied using nano-filtration and successive organic solvents extraction. Results indicated that the UV absorption intensity of nano-filtrated WWS was significantly enhanced. The UV absorption intensity of each extractive from WWS could be ranked as ether extractive (EE) > ethyl acetate extractive (EAE) > chloroform extractive (CE). Decoloration of crystal violet catalyzed by laccase/EE was higher than that by laccase/ABTS, which was 66.95% and 61.9% at 8 h, respectively. All the decoloration rates of malachite green at 60 min using EE, EAE and ABTS as mediator were both more than 80%. This research would benefit for broaden the source of laccase mediator and reduce the using cost of laccase/mediator system. (C) 2014 Elsevier Ltd. All rights reserved.
KeywordNatural Laccase Mediator Water-washed Solution Steam Exploded Corn Straw Nanofiltration Successive Organic Solvent Extraction
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.biortech.2014.01.044
URL查看原文
Indexed BySCI
Language英语
WOS KeywordBIOETHANOL PRODUCTION ; WHEAT-STRAW ; DECOLORIZATION ; PULP ; DYES ; TRIPHENYLMETHANE ; LIGNOCELLULOSE ; DETOXIFICATION ; RECALCITRANT ; TECHNOLOGIES
WOS Research AreaAgriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS SubjectAgricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS IDWOS:000334828500051
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/10953
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Qiu, Weihua,Zhang, Wenyan,Chen, Hongzhang. Natural laccase mediators separated from water-washed solution of steam exploded corn straw by nanofiltration and organic solvent fractionation[J]. BIORESOURCE TECHNOLOGY,2014,156(1):368-371.
APA Qiu, Weihua,Zhang, Wenyan,&Chen, Hongzhang.(2014).Natural laccase mediators separated from water-washed solution of steam exploded corn straw by nanofiltration and organic solvent fractionation.BIORESOURCE TECHNOLOGY,156(1),368-371.
MLA Qiu, Weihua,et al."Natural laccase mediators separated from water-washed solution of steam exploded corn straw by nanofiltration and organic solvent fractionation".BIORESOURCE TECHNOLOGY 156.1(2014):368-371.
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