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Microbial 2,3-Butanediol Production by Paenibacillus polymyxa Using Hemicelluloses Sugars from Steam Exploded Corn Stover
Alternative TitleJ. Biobased Mater. Bioenergy
Wang, Ning1,2; Chen, Hong-Zhang1
2014-06-01
Source PublicationJOURNAL OF BIOBASED MATERIALS AND BIOENERGY
ISSN1556-6560
Volume8Issue:3Pages:308-316
AbstractCurrently, the use of low-cost lignocellulosic biomass as an alternative substrate for 2,3-butanediol (2,3-BD) production to improve the economics of the process received considerable attention. But cellulose as the substrate required expensive cellulase, which increased the production costs. In this paper, hemicelluloses sugars were fractionated from corn stover by steam explosion. The objective of this study was to develop the corn stover hemicelluloses as carbon source to produce 2,3BD. After steam explosion, 69.81% of hemicelluloses were degraded to monosaccharide (glucose, xylose and arabinose) and oligosaccharide, meanwhile some by-products, such as acetic acid, furfural, 5-hydroxymethylfurfual (5-HMF) and soluble lignin compounds (SLC) were produced in the steam explosion process as well. Paenibacillus polymyxa S-07 could utilize all the hemicelluloses sugars to ferment 2,3-BD. Acetic acid could not only inhibit the production of by-products, but also promote the conversion of sugars to 2,3-BD. Compared with the fermentation without adding acetic acid, the concentration of 2,3-BD increased from 16.30 g/l to 22.41 g/l with adding 7 g/l acetic acid. Furfural and 5-HMF below 1 g/l promoted the production of 2,3-BD, and inhibited the production of 2,3-BD over 1 g/l. SLC was the inhibitor in the hemicelluloses extract. SLC over 2.62 g/l inhibited the growth of P. polymyxa S-07 severely. After detoxification of the extract with active carbon, SLC below 1.117 g/l almost had no effect on the production of 2,3-BD, the yield of 2,3-BD reached 12.07 g/l using the extract as carbon source, and the conversion rate of 2,3-BD was 0.42 g/g sugar. This study provides a potential feedstock for 2,3-butanediol production.; Currently, the use of low-cost lignocellulosic biomass as an alternative substrate for 2,3-butanediol (2,3-BD) production to improve the economics of the process received considerable attention. But cellulose as the substrate required expensive cellulase, which increased the production costs. In this paper, hemicelluloses sugars were fractionated from corn stover by steam explosion. The objective of this study was to develop the corn stover hemicelluloses as carbon source to produce 2,3BD. After steam explosion, 69.81% of hemicelluloses were degraded to monosaccharide (glucose, xylose and arabinose) and oligosaccharide, meanwhile some by-products, such as acetic acid, furfural, 5-hydroxymethylfurfual (5-HMF) and soluble lignin compounds (SLC) were produced in the steam explosion process as well. Paenibacillus polymyxa S-07 could utilize all the hemicelluloses sugars to ferment 2,3-BD. Acetic acid could not only inhibit the production of by-products, but also promote the conversion of sugars to 2,3-BD. Compared with the fermentation without adding acetic acid, the concentration of 2,3-BD increased from 16.30 g/l to 22.41 g/l with adding 7 g/l acetic acid. Furfural and 5-HMF below 1 g/l promoted the production of 2,3-BD, and inhibited the production of 2,3-BD over 1 g/l. SLC was the inhibitor in the hemicelluloses extract. SLC over 2.62 g/l inhibited the growth of P. polymyxa S-07 severely. After detoxification of the extract with active carbon, SLC below 1.117 g/l almost had no effect on the production of 2,3-BD, the yield of 2,3-BD reached 12.07 g/l using the extract as carbon source, and the conversion rate of 2,3-BD was 0.42 g/g sugar. This study provides a potential feedstock for 2,3-butanediol production.
KeywordBiorefinery Corn Stover Hemicelluloses Steam Explosion 2 3-butanediol
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
DOI10.1166/jbmb.2014.1436
URL查看原文
Indexed BySCI
Language英语
WOS KeywordASSISTED BIOMASS FRACTIONATION ; WHEAT-STRAW ; KLEBSIELLA-PNEUMONIAE ; BACILLUS-POLYMYXA ; ACID ; BIOREFINERIES ; FERMENTATION ; TECHNOLOGIES ; BIOETHANOL ; EXPLOSION
WOS Research AreaChemistry ; Energy & Fuels ; Materials Science
WOS SubjectChemistry, Applied ; Energy & Fuels ; Materials Science, Biomaterials
WOS IDWOS:000339422200004
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/10981
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wang, Ning,Chen, Hong-Zhang. Microbial 2,3-Butanediol Production by Paenibacillus polymyxa Using Hemicelluloses Sugars from Steam Exploded Corn Stover[J]. JOURNAL OF BIOBASED MATERIALS AND BIOENERGY,2014,8(3):308-316.
APA Wang, Ning,&Chen, Hong-Zhang.(2014).Microbial 2,3-Butanediol Production by Paenibacillus polymyxa Using Hemicelluloses Sugars from Steam Exploded Corn Stover.JOURNAL OF BIOBASED MATERIALS AND BIOENERGY,8(3),308-316.
MLA Wang, Ning,et al."Microbial 2,3-Butanediol Production by Paenibacillus polymyxa Using Hemicelluloses Sugars from Steam Exploded Corn Stover".JOURNAL OF BIOBASED MATERIALS AND BIOENERGY 8.3(2014):308-316.
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