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Novel Vaccine Delivery System Induces Robust Humoral and Cellular Immune Responses Based on Multiple Mechanisms
Alternative TitleAdv. Healthc. Mater.
Wang, Yue-Qi1,2; Wu, Jie1; Fan, Qing-Ze1,2; Zhou, Meng1; Yue, Zhan-Guo1,2; Ma, Guang-Hui1; Su, Zhi-Guo1
2014-05-01
Source PublicationADVANCED HEALTHCARE MATERIALS
ISSN2192-2640
Volume3Issue:5Pages:670-681
AbstractAiming to enhance the immunogenicity of H5N1 split vaccine, the development of a novel antigen delivery system based on quaternized chitosan hydrogel microparticles (Gel MPs) with multiple mechanisms of immunity enhancement is attempted. Gel MPs based on ionic cross-linking are prepared in a simple and mild way. Gel MPs are superior as a vaccine delivery system due to their ability to: 1) enhance cellular uptake and endosomal escape of antigens in dendritic cells (DCs); 2) significantly activate DCs; 3) form an antigen depot and recruit immunity cells to improve antigen capture. Further in vivo investigation shows that Gel MPs, in comparison to aluminum salts (Alum), LPS, and covalent cross-linking quaternized chitosan MPs (GC MPs), induce higher humoral and cellular immune responses with a mixed Th1/Th2 immunity. In conclusion, these results demonstrate that Gel MPs are efficient antigen delivery vehicles based on multiple mechanisms to enhance both humoral and cellular immune responses against H5N1 split antigen.; Aiming to enhance the immunogenicity of H5N1 split vaccine, the development of a novel antigen delivery system based on quaternized chitosan hydrogel microparticles (Gel MPs) with multiple mechanisms of immunity enhancement is attempted. Gel MPs based on ionic cross-linking are prepared in a simple and mild way. Gel MPs are superior as a vaccine delivery system due to their ability to: 1) enhance cellular uptake and endosomal escape of antigens in dendritic cells (DCs); 2) significantly activate DCs; 3) form an antigen depot and recruit immunity cells to improve antigen capture. Further in vivo investigation shows that Gel MPs, in comparison to aluminum salts (Alum), LPS, and covalent cross-linking quaternized chitosan MPs (GC MPs), induce higher humoral and cellular immune responses with a mixed Th1/Th2 immunity. In conclusion, these results demonstrate that Gel MPs are efficient antigen delivery vehicles based on multiple mechanisms to enhance both humoral and cellular immune responses against H5N1 split antigen.
KeywordHydrogels Particulate Delivery System Adjuvants Quaternized Chitosan H5n1 Split Vaccines
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
DOI10.1002/adhm.201300335
URL查看原文
Indexed BySCI
Language英语
WOS KeywordDENDRITIC CELLS ; INFLUENZA VACCINES ; VIRAL-INFECTION ; DRUG-DELIVERY ; H5N1 VIRUS ; IN-VITRO ; T-CELLS ; CHITOSAN ; ADJUVANT ; ACTIVATION
WOS Research AreaMaterials Science
WOS SubjectMaterials Science, Biomaterials
WOS IDWOS:000335730200005
Citation statistics
Cited Times:33[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/10986
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Key Lab Biopharmaceut Prod & Formulat Engn, Inst Proc Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wang, Yue-Qi,Wu, Jie,Fan, Qing-Ze,et al. Novel Vaccine Delivery System Induces Robust Humoral and Cellular Immune Responses Based on Multiple Mechanisms[J]. ADVANCED HEALTHCARE MATERIALS,2014,3(5):670-681.
APA Wang, Yue-Qi.,Wu, Jie.,Fan, Qing-Ze.,Zhou, Meng.,Yue, Zhan-Guo.,...&Su, Zhi-Guo.(2014).Novel Vaccine Delivery System Induces Robust Humoral and Cellular Immune Responses Based on Multiple Mechanisms.ADVANCED HEALTHCARE MATERIALS,3(5),670-681.
MLA Wang, Yue-Qi,et al."Novel Vaccine Delivery System Induces Robust Humoral and Cellular Immune Responses Based on Multiple Mechanisms".ADVANCED HEALTHCARE MATERIALS 3.5(2014):670-681.
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