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Improvement of the Production Efficiency of L-(+)-Tartaric Acid by Heterogeneous Whole-Cell Bioconversion
Alternative TitleAppl. Biochem. Biotechnol.
Wang, Ziqiang; Wang, Yunshan; Shi, Hong; Su, Zhiguo
2014-04-01
Source PublicationAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
ISSN0273-2289
Volume172Issue:8Pages:3989-4001
AbstractAn Escherichia coli-engineered bacterium with cis-epoxysuccinate hydrolase (ESH) activity was used to catalyze the stereospecific hydrolysis of cis-epoxysuccinic acid to l-(+)-tartaric acid. The effect of the substrate composition on the production efficiency of l-(+)-tartaric acid was investigated. Based on the sodium-type homogeneous substrate system, a heterogeneous substrate system, composed of 1.2 M sodium-type substrate and 1.8 M calcium-type substrate, was designed to improve ESH catalytic efficiency. After process optimization, a catalytic efficiency of 9.37 x 10(-3) g U-1 h(-1) was obtained with fed-batch mode in the heterogeneous substrate system, about a twofold increase compared to the traditional bioconversion process with Nocardia tartaricans cells. The scale-up tests were carried out in a 15-m(3) stirred tank reactor, which indicated that the heterogeneous substrate system had great application prospect for the l-(+)-tartaric acid industrial production.; An Escherichia coli-engineered bacterium with cis-epoxysuccinate hydrolase (ESH) activity was used to catalyze the stereospecific hydrolysis of cis-epoxysuccinic acid to l-(+)-tartaric acid. The effect of the substrate composition on the production efficiency of l-(+)-tartaric acid was investigated. Based on the sodium-type homogeneous substrate system, a heterogeneous substrate system, composed of 1.2 M sodium-type substrate and 1.8 M calcium-type substrate, was designed to improve ESH catalytic efficiency. After process optimization, a catalytic efficiency of 9.37 x 10(-3) g U-1 h(-1) was obtained with fed-batch mode in the heterogeneous substrate system, about a twofold increase compared to the traditional bioconversion process with Nocardia tartaricans cells. The scale-up tests were carried out in a 15-m(3) stirred tank reactor, which indicated that the heterogeneous substrate system had great application prospect for the l-(+)-tartaric acid industrial production.
KeywordL-(+)-tartaric Acid Cis-epoxysuccinate Hydrolase Heterogeneous Bioconversion Process Optimization Scale-up
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1007/s12010-014-0830-5
URL查看原文
Indexed BySCI
Language英语
WOS KeywordCIS-EPOXYSUCCINATE HYDROLASE ; PECTATE GEL BEADS ; EPOXIDE HYDROLASE ; NOCARDIA-TARTARICANS ; ESCHERICHIA-COLI ; EXPRESSION ; PURIFICATION ; ENTRAPMENT ; CULTURE ; CLONING
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS IDWOS:000334433200022
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Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/10989
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Wang, Ziqiang,Wang, Yunshan,Shi, Hong,et al. Improvement of the Production Efficiency of L-(+)-Tartaric Acid by Heterogeneous Whole-Cell Bioconversion[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2014,172(8):3989-4001.
APA Wang, Ziqiang,Wang, Yunshan,Shi, Hong,&Su, Zhiguo.(2014).Improvement of the Production Efficiency of L-(+)-Tartaric Acid by Heterogeneous Whole-Cell Bioconversion.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,172(8),3989-4001.
MLA Wang, Ziqiang,et al."Improvement of the Production Efficiency of L-(+)-Tartaric Acid by Heterogeneous Whole-Cell Bioconversion".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 172.8(2014):3989-4001.
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