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Preparation of the antithrombotic and antimicrobial coating through layer-by-layer self-assembly of nattokinase-nanosilver complex and polyethylenimine
Alternative TitleColloid Surf. B-Biointerfaces
Wei, Xuetuan1,2; Luo, Mingfang3; Liu, Huizhou2
2014-04-01
Source PublicationCOLLOIDS AND SURFACES B-BIOINTERFACES
ISSN0927-7765
Volume116Issue:1Pages:418-423
AbstractThe bifunctional coating with antithrombotic and antimicrobial activity was developed using nattokinase (NK) and nanosilver (AgNPs). Firstly, the adsorption interactions between NK and AgNPs were confirmed, and the composite particles of NK-AgNPs were prepared by adsorption of NK with AgNPs. At 5 FU/mL of NK concentration, the saturation adsorption capacity reached 24.35 FU/mg AgNPs with a high activity recovery of 97%, and adsorption by AgNPs also enhanced the heat stability and anticoagulant effect of NK. Based on the electrostatic force driven layer-by-layer self-assembly, the NK-AgNPs were further assembled with polyethylenimine (PEI) to form coating. UV-vis analysis showed that the self-assembly process was regular, and atom force microscopy analysis indicated that NK-AgNPs were uniformly embedded into the coating. The NK-AgNPs-PEI composite coating showed potent antithrombotic activity and antibacterial activity. This study developed a novel strategy to construct the bifunctional coating with antithrombotic and antimicrobial properties, and the coating material showed promising potential to be applied in the medical device. (C) 2014 Elsevier B.V. All rights reserved.; The bifunctional coating with antithrombotic and antimicrobial activity was developed using nattokinase (NK) and nanosilver (AgNPs). Firstly, the adsorption interactions between NK and AgNPs were confirmed, and the composite particles of NK-AgNPs were prepared by adsorption of NK with AgNPs. At 5 FU/mL of NK concentration, the saturation adsorption capacity reached 24.35 FU/mg AgNPs with a high activity recovery of 97%, and adsorption by AgNPs also enhanced the heat stability and anticoagulant effect of NK. Based on the electrostatic force driven layer-by-layer self-assembly, the NK-AgNPs were further assembled with polyethylenimine (PEI) to form coating. UV-vis analysis showed that the self-assembly process was regular, and atom force microscopy analysis indicated that NK-AgNPs were uniformly embedded into the coating. The NK-AgNPs-PEI composite coating showed potent antithrombotic activity and antibacterial activity. This study developed a novel strategy to construct the bifunctional coating with antithrombotic and antimicrobial properties, and the coating material showed promising potential to be applied in the medical device. (C) 2014 Elsevier B.V. All rights reserved.
KeywordNattokinase Nanosilver Antithrombotic Antimicrobial Coating
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences ; Technology
DOI10.1016/j.colsurfb.2014.01.034
URL查看原文
Indexed BySCI
Language英语
WOS KeywordHYDROPHILIC SURFACE-COATINGS ; SILVER NANOPARTICLES ; HEPARIN ; FOOD
WOS Research AreaBiophysics ; Chemistry ; Materials Science
WOS SubjectBiophysics ; Chemistry, Physical ; Materials Science, Biomaterials
WOS IDWOS:000335706100055
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/10990
Collection研究所(批量导入)
Affiliation1.Huazhong Agr Univ, Coll Food Sci & Technol, Minist Educ, Key Lab Environm Correlat Dietol, Wuhan 430070, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
3.Chinese Acad Sci, Inst Microbiol, Technol Transfer Ctr, Beijing 100101, Peoples R China
Recommended Citation
GB/T 7714
Wei, Xuetuan,Luo, Mingfang,Liu, Huizhou. Preparation of the antithrombotic and antimicrobial coating through layer-by-layer self-assembly of nattokinase-nanosilver complex and polyethylenimine[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2014,116(1):418-423.
APA Wei, Xuetuan,Luo, Mingfang,&Liu, Huizhou.(2014).Preparation of the antithrombotic and antimicrobial coating through layer-by-layer self-assembly of nattokinase-nanosilver complex and polyethylenimine.COLLOIDS AND SURFACES B-BIOINTERFACES,116(1),418-423.
MLA Wei, Xuetuan,et al."Preparation of the antithrombotic and antimicrobial coating through layer-by-layer self-assembly of nattokinase-nanosilver complex and polyethylenimine".COLLOIDS AND SURFACES B-BIOINTERFACES 116.1(2014):418-423.
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