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Inhibition of methemoglobin formation in aqueous solutions under aerobic conditions by the addition of amino acids
Alternative TitleInt. J. Biol. Macromol.
Wei, Yuping1,2; Li, Chunlong1,2; Zhang, Liang1,2; Su, Zhiguo1; Xu, Xia1
2014-03-01
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
Volume64Issue:1Pages:267-275
AbstractHemoglobin (Hb) as an important iron-containing oxygen-transport protein is easily oxidized to the ferric met-form, methemoglobin (metHb), and loses the capacity of binding oxygen during storage. In this study, the experimental data indicate that the presence of Tyr and Glu significantly suppress the metHb formation in the Hb solutions in aqueous environment under aerobic conditions at the temperature of 25 and 37 C, respectively. At pO(2) of 144 Torr the metHb percentage in the Hb solutions was the lowest with less than 10% at day 7 after incubation with Tyr at the ratio of 24 at pH 9.5 at 25 C. At 37 C, the metHb percentage did not reach 5% after 12 h of incubation with Glu at the ratio of 24 at pH 9. Molecular simulation analysis suggest that the presence of Tyr or Glu may contribute to the formation of the breakwater network, the stabilization of distal histidine, the changes in the size of heme pocket, and eventually result in the inhibition of metHb formation. This study provides insight into a new design for Hb-oxygen based carriers with strongly inhibition of metHb formation in aqueous environment under aerobic conditions, even at physiological temperature in vitro. (C) 2013 Elsevier B.V. All rights reserved.; Hemoglobin (Hb) as an important iron-containing oxygen-transport protein is easily oxidized to the ferric met-form, methemoglobin (metHb), and loses the capacity of binding oxygen during storage. In this study, the experimental data indicate that the presence of Tyr and Glu significantly suppress the metHb formation in the Hb solutions in aqueous environment under aerobic conditions at the temperature of 25 and 37 C, respectively. At pO(2) of 144 Torr the metHb percentage in the Hb solutions was the lowest with less than 10% at day 7 after incubation with Tyr at the ratio of 24 at pH 9.5 at 25 C. At 37 C, the metHb percentage did not reach 5% after 12 h of incubation with Glu at the ratio of 24 at pH 9. Molecular simulation analysis suggest that the presence of Tyr or Glu may contribute to the formation of the breakwater network, the stabilization of distal histidine, the changes in the size of heme pocket, and eventually result in the inhibition of metHb formation. This study provides insight into a new design for Hb-oxygen based carriers with strongly inhibition of metHb formation in aqueous environment under aerobic conditions, even at physiological temperature in vitro. (C) 2013 Elsevier B.V. All rights reserved.
KeywordAmino Acid Hemoglobin Methemoglobin Molecular Simulation Stability
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.ijbiomac.2013.12.010
URL查看原文
Indexed BySCI
Language英语
WOS KeywordHEMOGLOBIN-VESICLES ; OXYGEN CARRIERS ; IN-VIVO ; MOLECULAR-MECHANISM ; LIPID-PEROXIDATION ; CIRCULAR-DICHROISM ; BLOOD SUBSTITUTES ; HB VESICLES ; AUTOXIDATION ; REDUCTION
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000334147300040
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Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/10991
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wei, Yuping,Li, Chunlong,Zhang, Liang,et al. Inhibition of methemoglobin formation in aqueous solutions under aerobic conditions by the addition of amino acids[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2014,64(1):267-275.
APA Wei, Yuping,Li, Chunlong,Zhang, Liang,Su, Zhiguo,&Xu, Xia.(2014).Inhibition of methemoglobin formation in aqueous solutions under aerobic conditions by the addition of amino acids.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,64(1),267-275.
MLA Wei, Yuping,et al."Inhibition of methemoglobin formation in aqueous solutions under aerobic conditions by the addition of amino acids".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 64.1(2014):267-275.
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