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Design of novel cell penetrating peptides for the delivery of trehalose into mammalian cells
Alternative TitleBiochim. Biophys. Acta-Biomembr.
Wei, Yuping1,2; Li, Chunlong1,2; Zhang, Liang1,2; Xu, Xia1
2014-07-01
Source PublicationBIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
ISSN0005-2736
Volume1838Issue:7Pages:1911-1920
AbstractStabilization of cells in a desiccated state can significantly simplify the storage and transportation and save expenses for clinical applications. Introduction of the impermeable disaccharide, trehalose, into cells is an important step to improve the desiccation tolerance of cells. In this study, a novel cell penetrating peptide, KRKRWHW, was developed based on molecular simulations. The peptide exhibited little cytotoxicity and high penetrating efficiency into mammalian cells. The cell viability of mouse embryonic fibroblasts (MEFs) after the incubation with various concentrations of KRKRWHW from 0.01 mM to 5 mM at 37 degrees C for 4 h was maintained at around 100%. The peptide was able to penetrate into MEFs within 1 h at 37 degrees C with an efficiency of around 90% at 0.1 mM. Trehalose, as a cargo coupled with the peptide of KRKRWHW through hydrogen bond and pi-pi bond, was successfully loaded into the MEFs. This novel peptide provides a novel approach for the delivery of trehalose into mammalian cells. (C) 2014 Elsevier B.V. All rights reserved.; Stabilization of cells in a desiccated state can significantly simplify the storage and transportation and save expenses for clinical applications. Introduction of the impermeable disaccharide, trehalose, into cells is an important step to improve the desiccation tolerance of cells. In this study, a novel cell penetrating peptide, KRKRWHW, was developed based on molecular simulations. The peptide exhibited little cytotoxicity and high penetrating efficiency into mammalian cells. The cell viability of mouse embryonic fibroblasts (MEFs) after the incubation with various concentrations of KRKRWHW from 0.01 mM to 5 mM at 37 degrees C for 4 h was maintained at around 100%. The peptide was able to penetrate into MEFs within 1 h at 37 degrees C with an efficiency of around 90% at 0.1 mM. Trehalose, as a cargo coupled with the peptide of KRKRWHW through hydrogen bond and pi-pi bond, was successfully loaded into the MEFs. This novel peptide provides a novel approach for the delivery of trehalose into mammalian cells. (C) 2014 Elsevier B.V. All rights reserved.
KeywordCell Penetrating Peptide Trehalose Stabilization Lyophilization Mammalian Cell
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.bbamem.2014.02.011
URL查看原文
Indexed BySCI
Language英语
WOS KeywordMOLECULAR-DYNAMICS SIMULATION ; HIV-1 TAT PROTEIN ; PLASMA-MEMBRANE ; IN-VITRO ; INTRACELLULAR TREHALOSE ; DESICCATION TOLERANCE ; DRY STATE ; STABILIZATION ; ACID ; VIVO
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics
WOS SubjectBiochemistry & Molecular Biology ; Biophysics
WOS IDWOS:000336695300026
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/10992
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wei, Yuping,Li, Chunlong,Zhang, Liang,et al. Design of novel cell penetrating peptides for the delivery of trehalose into mammalian cells[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES,2014,1838(7):1911-1920.
APA Wei, Yuping,Li, Chunlong,Zhang, Liang,&Xu, Xia.(2014).Design of novel cell penetrating peptides for the delivery of trehalose into mammalian cells.BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES,1838(7),1911-1920.
MLA Wei, Yuping,et al."Design of novel cell penetrating peptides for the delivery of trehalose into mammalian cells".BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES 1838.7(2014):1911-1920.
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