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Membrane permeability of the human pluripotent stem cells to Me2SO, glycerol and 1,2-propanediol
Alternative TitleArch. Biochem. Biophys.
Xu, Yanqing1,2; Zhang, Liang1,2; Xu, Jiandong1; Wei, Yuping1,2; Xu, Xia1
2014-05-15
Source PublicationARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
ISSN0003-9861
Volume550Issue:1Pages:67-76
AbstractDue to the unlimited capacity of self-renew and ability to differentiate into derivatives of three germ layers, human pluripotent stem cells, including human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), have a great potential in regenerative medicine. A major challenge we are facing during the long-term storage of human pluripotent stem cells with the conventional slow cooling rate is the low cell recovery rate after cryopreservation which cannot meet the requirements for the future clinical applications. Evaluating the cell membrane permeability and the corresponding activation energy of hESCs and hiPSCs for water and different cryoprotective agents (CPA), including dimethyl sulfoxide (Me2SO), 1,2-propandiol and glycerol, is important for facilitating the development of cryopreservation protocol to enhance cell recovery after the cryopreservation. The osmotically inactive volume of hESCs and hiPSCs determined using the Boyle-van't Hoff model was 0.32V(0) and 0.42V(0), respectively. The membrane permeability was assessed from the volume changes of cells exposed to Me2SO, 1,2-propanediol and glycerol at the temperatures ranging from 8 to 30 degrees C. These results showed the biophysical differences between hESCs and hiPSCs. Their activation energy for water and CPAs extrapolated from the Arrhenius relationship indicated that the water transport was probably not through the channel-mediated mechanism. (C) 2014 Elsevier Inc. All rights reserved.; Due to the unlimited capacity of self-renew and ability to differentiate into derivatives of three germ layers, human pluripotent stem cells, including human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), have a great potential in regenerative medicine. A major challenge we are facing during the long-term storage of human pluripotent stem cells with the conventional slow cooling rate is the low cell recovery rate after cryopreservation which cannot meet the requirements for the future clinical applications. Evaluating the cell membrane permeability and the corresponding activation energy of hESCs and hiPSCs for water and different cryoprotective agents (CPA), including dimethyl sulfoxide (Me2SO), 1,2-propandiol and glycerol, is important for facilitating the development of cryopreservation protocol to enhance cell recovery after the cryopreservation. The osmotically inactive volume of hESCs and hiPSCs determined using the Boyle-van't Hoff model was 0.32V(0) and 0.42V(0), respectively. The membrane permeability was assessed from the volume changes of cells exposed to Me2SO, 1,2-propanediol and glycerol at the temperatures ranging from 8 to 30 degrees C. These results showed the biophysical differences between hESCs and hiPSCs. Their activation energy for water and CPAs extrapolated from the Arrhenius relationship indicated that the water transport was probably not through the channel-mediated mechanism. (C) 2014 Elsevier Inc. All rights reserved.
KeywordHuman Embryonic Stem Cells Human Induced Pluripotent Stem Cells Cryoprotectant Permeability Hydraulic Permeability Cryopreservation
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.abb.2014.04.010
URL查看原文
Indexed BySCI
Language英语
WOS KeywordRATIONALLY OPTIMIZED CRYOPRESERVATION ; INTRACELLULAR ICE FORMATION ; FREEZING-INJURY ; ROCK INHIBITOR ; HUMAN OOCYTES ; WATER ; KINETICS ; LINES ; DIMETHYLSULFOXIDE ; HYPOTHESIS
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics
WOS SubjectBiochemistry & Molecular Biology ; Biophysics
WOS IDWOS:000336780300009
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/11002
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Xu, Yanqing,Zhang, Liang,Xu, Jiandong,et al. Membrane permeability of the human pluripotent stem cells to Me2SO, glycerol and 1,2-propanediol[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,2014,550(1):67-76.
APA Xu, Yanqing,Zhang, Liang,Xu, Jiandong,Wei, Yuping,&Xu, Xia.(2014).Membrane permeability of the human pluripotent stem cells to Me2SO, glycerol and 1,2-propanediol.ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,550(1),67-76.
MLA Xu, Yanqing,et al."Membrane permeability of the human pluripotent stem cells to Me2SO, glycerol and 1,2-propanediol".ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 550.1(2014):67-76.
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