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Heat treatment increases the bioactivity of C-terminally PEGylated staphylokinase
Alternative TitleProcess Biochem.
Xue, Xiaoying1,2; Ji, Shaoyang1; Mu, Qimeng1,2; Hu, Tao1
2014-07-01
Source PublicationPROCESS BIOCHEMISTRY
ISSN1359-5113
Volume49Issue:7Pages:1092-1096
AbstractPEGylation can effectively improve the therapeutic potential of staphylokinase (SAK), a thrombolysis agent for therapy of myocardial infarction. However, polyethylene glycol (PEG) can sterically shield SAK and drastically decrease its bioactivity. In the present study, N-terminally PEGylated SAKs.(5 and 20 kDa PEG), C-terminally PEGylated SAKs with phenyl linker and the ones with amyl linker (5 and 20 kDa PEG) were prepared. The effects of the PEG length, the PEGylation site and linker chemistry on the bioactivity of the heat-treated PEGylated SAK were investigated. Heat treatment at 70 C for 2 h can improve the bioactivity of the C-terminally PEGylated SAKs, where the one with amyl linker and 20 kDa PEG showed the highest increase extent (27%) in the bioactivity. Thus, our study can advance the development of long-acting pharmaceutical protein with high bioactivity. (C) 2014 Elsevier Ltd. All rights reserved.; PEGylation can effectively improve the therapeutic potential of staphylokinase (SAK), a thrombolysis agent for therapy of myocardial infarction. However, polyethylene glycol (PEG) can sterically shield SAK and drastically decrease its bioactivity. In the present study, N-terminally PEGylated SAKs.(5 and 20 kDa PEG), C-terminally PEGylated SAKs with phenyl linker and the ones with amyl linker (5 and 20 kDa PEG) were prepared. The effects of the PEG length, the PEGylation site and linker chemistry on the bioactivity of the heat-treated PEGylated SAK were investigated. Heat treatment at 70 C for 2 h can improve the bioactivity of the C-terminally PEGylated SAKs, where the one with amyl linker and 20 kDa PEG showed the highest increase extent (27%) in the bioactivity. Thus, our study can advance the development of long-acting pharmaceutical protein with high bioactivity. (C) 2014 Elsevier Ltd. All rights reserved.
KeywordPolyethylene Glycol Staphylokinase Heat Treatment Bioactivity
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.procbio.2014.04.001
URL查看原文
Indexed BySCI
Language英语
WOS KeywordDRUG-DELIVERY ; STABILITY ; PROTEINS
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
WOS IDWOS:000338605500005
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/11003
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Xue, Xiaoying,Ji, Shaoyang,Mu, Qimeng,et al. Heat treatment increases the bioactivity of C-terminally PEGylated staphylokinase[J]. PROCESS BIOCHEMISTRY,2014,49(7):1092-1096.
APA Xue, Xiaoying,Ji, Shaoyang,Mu, Qimeng,&Hu, Tao.(2014).Heat treatment increases the bioactivity of C-terminally PEGylated staphylokinase.PROCESS BIOCHEMISTRY,49(7),1092-1096.
MLA Xue, Xiaoying,et al."Heat treatment increases the bioactivity of C-terminally PEGylated staphylokinase".PROCESS BIOCHEMISTRY 49.7(2014):1092-1096.
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