CAS OpenIR  > 研究所(批量导入)
Construction of reductive pathway in Saccharomyces cerevisiae for effective succinic acid fermentation at low pH value
Alternative TitleBioresour. Technol.
Yan, Daojiang1,2; Wang, Caixia1,2; Zhou, Jiemin1,2; Liu, Yilan1,2; Yang, Maohua1; Xing, Jianmin1
2014-03-01
Source PublicationBIORESOURCE TECHNOLOGY
ISSN0960-8524
Volume156Issue:1Pages:232-239
AbstractSuccinic acid is an important precursor for the synthesis of high-value-added products. Saccharomyces cerevisiae is a suitable platform for succinic acid production because of its high tolerance towards acidity. In this study, a modified pathway for succinate production was established and investigated in S. cerevisiae. The engineered strain could produce up to 6.17 +/- 0.34 g/L of succinate through the constructed pathway. The succinate titer was further improved to 8.09 +/- 0.28 g/L by the deletion of GPD1 and even higher to 9.98 +/- 0.23 g/L with a yield of 0.32 mol/mol glucose through regulation of biotin and urea levels. Under optimal supplemental CO2 conditions in a bioreactor, the engineered strain produced 12.97 +/- 0.42 g/L succinate with a yield of 0.21 mol/mol glucose at pH 3.8. These results demonstrated that the proposed engineering strategy was efficient for succinic acid production at low pH value. (C) 2014 Elsevier Ltd. All rights reserved.; Succinic acid is an important precursor for the synthesis of high-value-added products. Saccharomyces cerevisiae is a suitable platform for succinic acid production because of its high tolerance towards acidity. In this study, a modified pathway for succinate production was established and investigated in S. cerevisiae. The engineered strain could produce up to 6.17 +/- 0.34 g/L of succinate through the constructed pathway. The succinate titer was further improved to 8.09 +/- 0.28 g/L by the deletion of GPD1 and even higher to 9.98 +/- 0.23 g/L with a yield of 0.32 mol/mol glucose through regulation of biotin and urea levels. Under optimal supplemental CO2 conditions in a bioreactor, the engineered strain produced 12.97 +/- 0.42 g/L succinate with a yield of 0.21 mol/mol glucose at pH 3.8. These results demonstrated that the proposed engineering strategy was efficient for succinic acid production at low pH value. (C) 2014 Elsevier Ltd. All rights reserved.
KeywordSuccinic Acid Reductive Pathway Reducing Power Low Ph Saccharomyces Cerevisiae
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.biortech.2014.01.053
URL查看原文
Indexed BySCI
Language英语
WOS KeywordCHEMOSTAT CULTURES ; ESCHERICHIA-COLI ; PYRUVATE ; GENE ; ISOENZYMES ; EVOLUTION ; FUMARASE ; GLUCOSE
WOS Research AreaAgriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS SubjectAgricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS IDWOS:000334828500032
Citation statistics
Cited Times:56[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/11004
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Yan, Daojiang,Wang, Caixia,Zhou, Jiemin,et al. Construction of reductive pathway in Saccharomyces cerevisiae for effective succinic acid fermentation at low pH value[J]. BIORESOURCE TECHNOLOGY,2014,156(1):232-239.
APA Yan, Daojiang,Wang, Caixia,Zhou, Jiemin,Liu, Yilan,Yang, Maohua,&Xing, Jianmin.(2014).Construction of reductive pathway in Saccharomyces cerevisiae for effective succinic acid fermentation at low pH value.BIORESOURCE TECHNOLOGY,156(1),232-239.
MLA Yan, Daojiang,et al."Construction of reductive pathway in Saccharomyces cerevisiae for effective succinic acid fermentation at low pH value".BIORESOURCE TECHNOLOGY 156.1(2014):232-239.
Files in This Item:
File Name/Size DocType Version Access License
Construction of redu(543KB) 限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yan, Daojiang]'s Articles
[Wang, Caixia]'s Articles
[Zhou, Jiemin]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yan, Daojiang]'s Articles
[Wang, Caixia]'s Articles
[Zhou, Jiemin]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yan, Daojiang]'s Articles
[Wang, Caixia]'s Articles
[Zhou, Jiemin]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.