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On-line modeling intracellular carbon and energy metabolism of Nannochloropsis sp. in nitrogen-repletion and nitrogen-limitation cultures
Alternative TitleBioresour. Technol.
Zhang, Dongmei1,2; Yan, Fei1,2; Sun, Zhongliang1,2; Zhang, Qinghua1; Xue, Shengzhang1; Cong, Wei1
2014-07-01
Source PublicationBIORESOURCE TECHNOLOGY
ISSN0960-8524
Volume164Issue:1Pages:86-92
AbstractIn this study, a photobioreactor cultivation system and a calculation method for on-line monitoring of carbon and energy metabolism of microalgae were developed using Nannochloropsis sp. in nitrogen-repletion and nitrogen-limitation cultures. Only 30-60% of carbon fixed in Calvin cycle was used for biomass and the rest was lost in light respiration. The net fixed carbon was assumed to be incorporated into protein, lipids, carbohydrates, and nucleic acids, whose contents calculated on-line fitted well with the experimental measurements. Intracellular ATPs were quantitatively divided for biomass production and cell maintenance, and the result is in accordance with known reports. Due to light limitation induced by high cell concentration in batch cultures, the proportion of CO2 loss in light respiration and the proportion of energy for maintenance rapidly increased in culturing process. Nitrogen starvation reduced the light respiration, thus decreasing CO2 loss and maintenance energy, but no effect on ATP requirement for cell growth. (C) 2014 Elsevier Ltd. All rights reserved.; In this study, a photobioreactor cultivation system and a calculation method for on-line monitoring of carbon and energy metabolism of microalgae were developed using Nannochloropsis sp. in nitrogen-repletion and nitrogen-limitation cultures. Only 30-60% of carbon fixed in Calvin cycle was used for biomass and the rest was lost in light respiration. The net fixed carbon was assumed to be incorporated into protein, lipids, carbohydrates, and nucleic acids, whose contents calculated on-line fitted well with the experimental measurements. Intracellular ATPs were quantitatively divided for biomass production and cell maintenance, and the result is in accordance with known reports. Due to light limitation induced by high cell concentration in batch cultures, the proportion of CO2 loss in light respiration and the proportion of energy for maintenance rapidly increased in culturing process. Nitrogen starvation reduced the light respiration, thus decreasing CO2 loss and maintenance energy, but no effect on ATP requirement for cell growth. (C) 2014 Elsevier Ltd. All rights reserved.
KeywordMicroalgae Carbon Metabolism Energy Metabolism On-line Monitor Biomass Composition
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.biortech.2014.04.083
URL查看原文
Indexed BySCI
Language英语
WOS KeywordCHLAMYDOMONAS-REINHARDTII ; LIPID-ACCUMULATION ; LIGHT-INTENSITY ; GROWTH-RATES ; BIOMASS ; PHOTOBIOREACTOR ; MICROALGAE ; TEMPERATURE ; STARVATION ; NUTRIENTS
WOS Research AreaAgriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS SubjectAgricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS IDWOS:000338710500013
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/11025
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Dongmei,Yan, Fei,Sun, Zhongliang,et al. On-line modeling intracellular carbon and energy metabolism of Nannochloropsis sp. in nitrogen-repletion and nitrogen-limitation cultures[J]. BIORESOURCE TECHNOLOGY,2014,164(1):86-92.
APA Zhang, Dongmei,Yan, Fei,Sun, Zhongliang,Zhang, Qinghua,Xue, Shengzhang,&Cong, Wei.(2014).On-line modeling intracellular carbon and energy metabolism of Nannochloropsis sp. in nitrogen-repletion and nitrogen-limitation cultures.BIORESOURCE TECHNOLOGY,164(1),86-92.
MLA Zhang, Dongmei,et al."On-line modeling intracellular carbon and energy metabolism of Nannochloropsis sp. in nitrogen-repletion and nitrogen-limitation cultures".BIORESOURCE TECHNOLOGY 164.1(2014):86-92.
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