Knowledge Management System Of Institute of process engineering,CAS
| Efficient resveratrol production by immobilized beta-glucosidase on cross-linked chitosan microsphere modified by L-lysine | |
| Alternative Title | J. Mol. Catal. B-Enzym. |
| Zhang, Jie1; Wang, Dan1; Pan, Jiachuan2; Wang, Jing1; Zhao, Hongbo1; Li, Qiang3; Zhou, Xiaohua1 | |
| 2014-06-01 | |
| Source Publication | JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
 |
| ISSN | 1381-1177 |
| Volume | 104Issue:1Pages:29-34 |
| Abstract | Resveratrol and its glycosidic derivative polydatin are the major stilbenes in Polygonum cuspidatum, a most widely used traditional herbal medicine. To produce resveratrol via enzymatic hydrolysis of polydatin, beta-glucosidase was immobilized on cross-linked chitosan microspheres modified by L-lysine. The condition of enzyme immobilization was optimized, and the characterization of the immobilized enzyme was carried out. K-m value of immobilized beta-glucosidase was calculated as 47.7 +/- 0.7 mM, and V-max value was 34.6 +/- 0.5 U/mg. Under optimized condition, which was 45 degrees C, pH 6.0, with the initial polydatin concentration of 2.0%, and the flow rate of 1.0 mL/min, the overall hydrolysis yield could reach 85.8 +/- 0.6%, and the activity of the immobilized enzyme remained 92.2 +/- 0.9% after continuous hydrolysis for 8 h. Enzymatic hydrolysis of polydatin extracted from P. cuspidatum is a promising method to increase the supply of resveratrol in pharmaceutical markets worldwide, which also showed a new application of immobilized beta-glucosidase in biotechnological industry. (C) 2014 Elsevier B.V. All rights reserved.; Resveratrol and its glycosidic derivative polydatin are the major stilbenes in Polygonum cuspidatum, a most widely used traditional herbal medicine. To produce resveratrol via enzymatic hydrolysis of polydatin, beta-glucosidase was immobilized on cross-linked chitosan microspheres modified by L-lysine. The condition of enzyme immobilization was optimized, and the characterization of the immobilized enzyme was carried out. K-m value of immobilized beta-glucosidase was calculated as 47.7 +/- 0.7 mM, and V-max value was 34.6 +/- 0.5 U/mg. Under optimized condition, which was 45 degrees C, pH 6.0, with the initial polydatin concentration of 2.0%, and the flow rate of 1.0 mL/min, the overall hydrolysis yield could reach 85.8 +/- 0.6%, and the activity of the immobilized enzyme remained 92.2 +/- 0.9% after continuous hydrolysis for 8 h. Enzymatic hydrolysis of polydatin extracted from P. cuspidatum is a promising method to increase the supply of resveratrol in pharmaceutical markets worldwide, which also showed a new application of immobilized beta-glucosidase in biotechnological industry. (C) 2014 Elsevier B.V. All rights reserved. |
| Keyword | Resveratrol Beta-glucosidase Immobilization Cross-linked Chitosan Microsphere L-lysine |
| Subtype | Article |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| DOI | 10.1016/j.molcatb.2014.03.003 |
| URL | 查看原文 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | TRANS-RESVERATROL ; ALPHA-GLUCOSIDASE ; CLAY COMPOSITE ; STABILITY ; WATER ; WINE |
| WOS Research Area | Biochemistry & Molecular Biology ; Chemistry |
| WOS Subject | Biochemistry & Molecular Biology ; Chemistry, Physical |
| WOS ID | WOS:000336779700005 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Version | 出版稿 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/11028 |
| Collection | 研究所（批量导入） |
| Affiliation | 1.Chongqing Univ, Coll Chem & Chem Engn, Chongqing 400044, Peoples R China 2.Syracuse Univ, Dept Biomed & Chem Engn, Syracuse, NY 13244 USA 3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| Recommended Citation GB/T 7714 | Zhang, Jie,Wang, Dan,Pan, Jiachuan,et al. Efficient resveratrol production by immobilized beta-glucosidase on cross-linked chitosan microsphere modified by L-lysine[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,2014,104(1):29-34. |
| APA | Zhang, Jie.,Wang, Dan.,Pan, Jiachuan.,Wang, Jing.,Zhao, Hongbo.,...&Zhou, Xiaohua.(2014).Efficient resveratrol production by immobilized beta-glucosidase on cross-linked chitosan microsphere modified by L-lysine.JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,104(1),29-34. |
| MLA | Zhang, Jie,et al."Efficient resveratrol production by immobilized beta-glucosidase on cross-linked chitosan microsphere modified by L-lysine".JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 104.1(2014):29-34. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Efficient resveratro（626KB） | 限制开放 | CC BY-NC-SA | Application Full Text | |||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment