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Efficient resveratrol production by immobilized beta-glucosidase on cross-linked chitosan microsphere modified by L-lysine
Alternative TitleJ. Mol. Catal. B-Enzym.
Zhang, Jie1; Wang, Dan1; Pan, Jiachuan2; Wang, Jing1; Zhao, Hongbo1; Li, Qiang3; Zhou, Xiaohua1
2014-06-01
Source PublicationJOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
ISSN1381-1177
Volume104Issue:1Pages:29-34
AbstractResveratrol and its glycosidic derivative polydatin are the major stilbenes in Polygonum cuspidatum, a most widely used traditional herbal medicine. To produce resveratrol via enzymatic hydrolysis of polydatin, beta-glucosidase was immobilized on cross-linked chitosan microspheres modified by L-lysine. The condition of enzyme immobilization was optimized, and the characterization of the immobilized enzyme was carried out. K-m value of immobilized beta-glucosidase was calculated as 47.7 +/- 0.7 mM, and V-max value was 34.6 +/- 0.5 U/mg. Under optimized condition, which was 45 degrees C, pH 6.0, with the initial polydatin concentration of 2.0%, and the flow rate of 1.0 mL/min, the overall hydrolysis yield could reach 85.8 +/- 0.6%, and the activity of the immobilized enzyme remained 92.2 +/- 0.9% after continuous hydrolysis for 8 h. Enzymatic hydrolysis of polydatin extracted from P. cuspidatum is a promising method to increase the supply of resveratrol in pharmaceutical markets worldwide, which also showed a new application of immobilized beta-glucosidase in biotechnological industry. (C) 2014 Elsevier B.V. All rights reserved.; Resveratrol and its glycosidic derivative polydatin are the major stilbenes in Polygonum cuspidatum, a most widely used traditional herbal medicine. To produce resveratrol via enzymatic hydrolysis of polydatin, beta-glucosidase was immobilized on cross-linked chitosan microspheres modified by L-lysine. The condition of enzyme immobilization was optimized, and the characterization of the immobilized enzyme was carried out. K-m value of immobilized beta-glucosidase was calculated as 47.7 +/- 0.7 mM, and V-max value was 34.6 +/- 0.5 U/mg. Under optimized condition, which was 45 degrees C, pH 6.0, with the initial polydatin concentration of 2.0%, and the flow rate of 1.0 mL/min, the overall hydrolysis yield could reach 85.8 +/- 0.6%, and the activity of the immobilized enzyme remained 92.2 +/- 0.9% after continuous hydrolysis for 8 h. Enzymatic hydrolysis of polydatin extracted from P. cuspidatum is a promising method to increase the supply of resveratrol in pharmaceutical markets worldwide, which also showed a new application of immobilized beta-glucosidase in biotechnological industry. (C) 2014 Elsevier B.V. All rights reserved.
KeywordResveratrol Beta-glucosidase Immobilization Cross-linked Chitosan Microsphere L-lysine
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.molcatb.2014.03.003
URL查看原文
Indexed BySCI
Language英语
WOS KeywordTRANS-RESVERATROL ; ALPHA-GLUCOSIDASE ; CLAY COMPOSITE ; STABILITY ; WATER ; WINE
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Physical
WOS IDWOS:000336779700005
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/11028
Collection研究所(批量导入)
Affiliation1.Chongqing Univ, Coll Chem & Chem Engn, Chongqing 400044, Peoples R China
2.Syracuse Univ, Dept Biomed & Chem Engn, Syracuse, NY 13244 USA
3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Jie,Wang, Dan,Pan, Jiachuan,et al. Efficient resveratrol production by immobilized beta-glucosidase on cross-linked chitosan microsphere modified by L-lysine[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,2014,104(1):29-34.
APA Zhang, Jie.,Wang, Dan.,Pan, Jiachuan.,Wang, Jing.,Zhao, Hongbo.,...&Zhou, Xiaohua.(2014).Efficient resveratrol production by immobilized beta-glucosidase on cross-linked chitosan microsphere modified by L-lysine.JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,104(1),29-34.
MLA Zhang, Jie,et al."Efficient resveratrol production by immobilized beta-glucosidase on cross-linked chitosan microsphere modified by L-lysine".JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 104.1(2014):29-34.
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