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Efficient resveratrol production by immobilized beta-glucosidase on cross-linked chitosan microsphere modified by L-lysine | |
Alternative Title | J. Mol. Catal. B-Enzym. |
Zhang, Jie1; Wang, Dan1; Pan, Jiachuan2; Wang, Jing1; Zhao, Hongbo1; Li, Qiang3; Zhou, Xiaohua1 | |
2014-06-01 | |
Source Publication | JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
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ISSN | 1381-1177 |
Volume | 104Issue:1Pages:29-34 |
Abstract | Resveratrol and its glycosidic derivative polydatin are the major stilbenes in Polygonum cuspidatum, a most widely used traditional herbal medicine. To produce resveratrol via enzymatic hydrolysis of polydatin, beta-glucosidase was immobilized on cross-linked chitosan microspheres modified by L-lysine. The condition of enzyme immobilization was optimized, and the characterization of the immobilized enzyme was carried out. K-m value of immobilized beta-glucosidase was calculated as 47.7 +/- 0.7 mM, and V-max value was 34.6 +/- 0.5 U/mg. Under optimized condition, which was 45 degrees C, pH 6.0, with the initial polydatin concentration of 2.0%, and the flow rate of 1.0 mL/min, the overall hydrolysis yield could reach 85.8 +/- 0.6%, and the activity of the immobilized enzyme remained 92.2 +/- 0.9% after continuous hydrolysis for 8 h. Enzymatic hydrolysis of polydatin extracted from P. cuspidatum is a promising method to increase the supply of resveratrol in pharmaceutical markets worldwide, which also showed a new application of immobilized beta-glucosidase in biotechnological industry. (C) 2014 Elsevier B.V. All rights reserved.; Resveratrol and its glycosidic derivative polydatin are the major stilbenes in Polygonum cuspidatum, a most widely used traditional herbal medicine. To produce resveratrol via enzymatic hydrolysis of polydatin, beta-glucosidase was immobilized on cross-linked chitosan microspheres modified by L-lysine. The condition of enzyme immobilization was optimized, and the characterization of the immobilized enzyme was carried out. K-m value of immobilized beta-glucosidase was calculated as 47.7 +/- 0.7 mM, and V-max value was 34.6 +/- 0.5 U/mg. Under optimized condition, which was 45 degrees C, pH 6.0, with the initial polydatin concentration of 2.0%, and the flow rate of 1.0 mL/min, the overall hydrolysis yield could reach 85.8 +/- 0.6%, and the activity of the immobilized enzyme remained 92.2 +/- 0.9% after continuous hydrolysis for 8 h. Enzymatic hydrolysis of polydatin extracted from P. cuspidatum is a promising method to increase the supply of resveratrol in pharmaceutical markets worldwide, which also showed a new application of immobilized beta-glucosidase in biotechnological industry. (C) 2014 Elsevier B.V. All rights reserved. |
Keyword | Resveratrol Beta-glucosidase Immobilization Cross-linked Chitosan Microsphere L-lysine |
Subtype | Article |
WOS Headings | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
DOI | 10.1016/j.molcatb.2014.03.003 |
URL | 查看原文 |
Indexed By | SCI |
Language | 英语 |
WOS Keyword | TRANS-RESVERATROL ; ALPHA-GLUCOSIDASE ; CLAY COMPOSITE ; STABILITY ; WATER ; WINE |
WOS Research Area | Biochemistry & Molecular Biology ; Chemistry |
WOS Subject | Biochemistry & Molecular Biology ; Chemistry, Physical |
WOS ID | WOS:000336779700005 |
Citation statistics | |
Document Type | 期刊论文 |
Version | 出版稿 |
Identifier | http://ir.ipe.ac.cn/handle/122111/11028 |
Collection | 研究所(批量导入) |
Affiliation | 1.Chongqing Univ, Coll Chem & Chem Engn, Chongqing 400044, Peoples R China 2.Syracuse Univ, Dept Biomed & Chem Engn, Syracuse, NY 13244 USA 3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
Recommended Citation GB/T 7714 | Zhang, Jie,Wang, Dan,Pan, Jiachuan,et al. Efficient resveratrol production by immobilized beta-glucosidase on cross-linked chitosan microsphere modified by L-lysine[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,2014,104(1):29-34. |
APA | Zhang, Jie.,Wang, Dan.,Pan, Jiachuan.,Wang, Jing.,Zhao, Hongbo.,...&Zhou, Xiaohua.(2014).Efficient resveratrol production by immobilized beta-glucosidase on cross-linked chitosan microsphere modified by L-lysine.JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,104(1),29-34. |
MLA | Zhang, Jie,et al."Efficient resveratrol production by immobilized beta-glucosidase on cross-linked chitosan microsphere modified by L-lysine".JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 104.1(2014):29-34. |
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