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Hydrophilic modification gigaporous resins with poly(ethylenimine) for high-throughput proteins ion-exchange chromatography
Alternative TitleJ. Chromatogr. A
Zhang, Rongyue; Li, Qiang; Gao, Yang; Li, Juan; Huang, Yongdong; Song, Cui; Zhou, Weiqing; Ma, Guanghui; Su, Zhiguo
2014-05-23
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1343Issue:1Pages:109-118
AbstractHigh hydrophilicity of gigaporous microspheres based on a copolymer of poly(glycidyl methacrylate)-co-divinyl benzene (PGMA-DVB) was successfully realized through coating the branched polyethyleneimine (PEI) in PGMA-DVB microspheres. PEI with various molecules weights and different branching agents were identified in terms of protein recovery as evaluation approach. For this evaluation, PEI600 (M-w = 600) and poly (ethylene glycol) diglycidyl ether (PEGDE, M-w = 400) were used as modification agent and branching agent, respectively. The modified microspheres showed good permeability and revealed a certain mechanical strength. After modification, the protein recovery increased from 40% to >90%. The protein recovery increased with the branched generations and the first and second generations could give the protein recovery of 93% and 96%, respectively. Meanwhile, the ionic capacity also showed a rising trend in the range of 0.11-0.32 mmol/mL with the branched generations. But the dynamic binding capacity of protein (bovine serum albumin, BSA as the model protein) increased at first and then decreased. Analysis of the dry microspheres structure by mercury intrusion method as well as observation of the branched PEI on PGMA-DVB membrane in aqueous solution indicated that excess PEI chains with the extended state in the second generation would block the small pores and decrease the accessible surface area. Therefore, the protein capacity on the second generation, on the contrary, was lower than that on the first generation. Meanwhile, it was found that the PEI chains in the modified microspheres changed their construction from the extended to the collapsed state with increase of NaCl concentration. And the corresponding pore size of the modified microspheres increased with salt concentration through low-field nuclear magnetic resonance. Dynamic binding capacity of proteins on the modified supports did not significantly change with increase of the flow rate. The media showed good performance for separation three model proteins at high flow rate of 1084 cm/h. This modified gigaporous microspheres had a large potential in application for rapid separation of biomolecules. (C) 2014 Elsevier B.V. All rights reserved.; High hydrophilicity of gigaporous microspheres based on a copolymer of poly(glycidyl methacrylate)-co-divinyl benzene (PGMA-DVB) was successfully realized through coating the branched polyethyleneimine (PEI) in PGMA-DVB microspheres. PEI with various molecules weights and different branching agents were identified in terms of protein recovery as evaluation approach. For this evaluation, PEI600 (M-w = 600) and poly (ethylene glycol) diglycidyl ether (PEGDE, M-w = 400) were used as modification agent and branching agent, respectively. The modified microspheres showed good permeability and revealed a certain mechanical strength. After modification, the protein recovery increased from 40% to >90%. The protein recovery increased with the branched generations and the first and second generations could give the protein recovery of 93% and 96%, respectively. Meanwhile, the ionic capacity also showed a rising trend in the range of 0.11-0.32 mmol/mL with the branched generations. But the dynamic binding capacity of protein (bovine serum albumin, BSA as the model protein) increased at first and then decreased. Analysis of the dry microspheres structure by mercury intrusion method as well as observation of the branched PEI on PGMA-DVB membrane in aqueous solution indicated that excess PEI chains with the extended state in the second generation would block the small pores and decrease the accessible surface area. Therefore, the protein capacity on the second generation, on the contrary, was lower than that on the first generation. Meanwhile, it was found that the PEI chains in the modified microspheres changed their construction from the extended to the collapsed state with increase of NaCl concentration. And the corresponding pore size of the modified microspheres increased with salt concentration through low-field nuclear magnetic resonance. Dynamic binding capacity of proteins on the modified supports did not significantly change with increase of the flow rate. The media showed good performance for separation three model proteins at high flow rate of 1084 cm/h. This modified gigaporous microspheres had a large potential in application for rapid separation of biomolecules. (C) 2014 Elsevier B.V. All rights reserved.
KeywordHydrophilicity Gigaporous Microspheres Anion-exchange Medium Branched Polyethyleneimine High-throughput
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.chroma.2014.03.064
URL查看原文
Indexed BySCI
Language英语
WOS KeywordTRANSFER RADICAL POLYMERIZATION ; MICELLES SWELLING METHOD ; HIGH-CAPACITY ; CAPILLARY ELECTROCHROMATOGRAPHY ; POLY(GLYCIDYL METHACRYLATE) ; PERFUSION CHROMATOGRAPHY ; POLYSTYRENE MICROSPHERES ; LIQUID-CHROMATOGRAPHY ; STATIONARY PHASES ; RAPID SEPARATION
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000336195800014
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Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/11032
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Rongyue,Li, Qiang,Gao, Yang,et al. Hydrophilic modification gigaporous resins with poly(ethylenimine) for high-throughput proteins ion-exchange chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1343(1):109-118.
APA Zhang, Rongyue.,Li, Qiang.,Gao, Yang.,Li, Juan.,Huang, Yongdong.,...&Su, Zhiguo.(2014).Hydrophilic modification gigaporous resins with poly(ethylenimine) for high-throughput proteins ion-exchange chromatography.JOURNAL OF CHROMATOGRAPHY A,1343(1),109-118.
MLA Zhang, Rongyue,et al."Hydrophilic modification gigaporous resins with poly(ethylenimine) for high-throughput proteins ion-exchange chromatography".JOURNAL OF CHROMATOGRAPHY A 1343.1(2014):109-118.
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