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Immune responses to vaccines involving a combined antigen-nanoparticle mixture and nanoparticle-encapsulated antigen formulation
Alternative TitleBiomaterials
Zhang, Weifeng1,2; Wang, Lianyan1; Liu, Yuan1,2; Chen, Xiaoming1,2; Liu, Qi1,2; Jia, Jilei1,2; Yang, Tingyuan1; Qiu, Shaohui3; Ma, Guanghui1
2014-07-01
Source PublicationBIOMATERIALS
ISSN0142-9612
Volume35Issue:23Pages:6086-6097
AbstractMany physicochemical characteristics significantly influence the adjuvant effect of micro/nanoparticles; one critical factor is the kinetics of antigen exposure to the immune system by particle-adjuvanted vaccines. Here, we investigated how various antigen-nanoparticle formulations impacted antigen exposure to the immune system and the resultant antigen-specific immune responses. We formulated antigen with poly(lactic-co-glycolic acid) (PLGA) nanoparticles by encapsulating antigen within nanoparticles or by simply mixing soluble antigen with the nanoparticles. Our results indicated that the combined formulation (composed of antigen encapsulated in nanoparticles and antigen mixed with nanoparticles) induced more powerful antigen-specific immune responses than each single-component formulation. Mice immunized with the combined vaccine formulation displayed enhanced induction of antigen-specific IgG antibodies with high avidity, increased cytokine secretion by splenocytes, and improved generation of memory T cell. Enhanced immune responses elicited by the combined vaccine formulation might be attributed to the antigen-depot effect at the injection site, effective provision of both adequate initial antigen exposure and long-term antigen persistence, and efficient induction of dendritic cell (DC) activation and follicular helper T cell differentiation in draining lymph nodes. Understanding the effect of antigen nanoparticle formulations on the resultant immune responses might have significant implications for rational vaccine design. (C) 2014 Elsevier Ltd. All rights reserved.; Many physicochemical characteristics significantly influence the adjuvant effect of micro/nanoparticles; one critical factor is the kinetics of antigen exposure to the immune system by particle-adjuvanted vaccines. Here, we investigated how various antigen-nanoparticle formulations impacted antigen exposure to the immune system and the resultant antigen-specific immune responses. We formulated antigen with poly(lactic-co-glycolic acid) (PLGA) nanoparticles by encapsulating antigen within nanoparticles or by simply mixing soluble antigen with the nanoparticles. Our results indicated that the combined formulation (composed of antigen encapsulated in nanoparticles and antigen mixed with nanoparticles) induced more powerful antigen-specific immune responses than each single-component formulation. Mice immunized with the combined vaccine formulation displayed enhanced induction of antigen-specific IgG antibodies with high avidity, increased cytokine secretion by splenocytes, and improved generation of memory T cell. Enhanced immune responses elicited by the combined vaccine formulation might be attributed to the antigen-depot effect at the injection site, effective provision of both adequate initial antigen exposure and long-term antigen persistence, and efficient induction of dendritic cell (DC) activation and follicular helper T cell differentiation in draining lymph nodes. Understanding the effect of antigen nanoparticle formulations on the resultant immune responses might have significant implications for rational vaccine design. (C) 2014 Elsevier Ltd. All rights reserved.
KeywordAdjuvant Nanoparticles Vaccine Formulations Immune Response Mechanism Of Action
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
DOI10.1016/j.biomaterials.2014.04.022
URL查看原文
Indexed BySCI
Language英语
WOS KeywordT-CELL RESPONSE ; PARTICLE-SIZE ; ADJUVANTS ; DELIVERY ; MICROPARTICLES ; KINETICS ; PROMOTE ; DIFFERENTIATION ; HYDROPHOBICITY ; PERSISTENCE
WOS Research AreaEngineering ; Materials Science
WOS SubjectEngineering, Biomedical ; Materials Science, Biomaterials
WOS IDWOS:000337212200011
Citation statistics
Cited Times:78[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/11036
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, PLA Key Lab Biopharmaceut Prod & Formulat Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Natl Inst Food & Drug Control, Beijing 100050, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Weifeng,Wang, Lianyan,Liu, Yuan,et al. Immune responses to vaccines involving a combined antigen-nanoparticle mixture and nanoparticle-encapsulated antigen formulation[J]. BIOMATERIALS,2014,35(23):6086-6097.
APA Zhang, Weifeng.,Wang, Lianyan.,Liu, Yuan.,Chen, Xiaoming.,Liu, Qi.,...&Ma, Guanghui.(2014).Immune responses to vaccines involving a combined antigen-nanoparticle mixture and nanoparticle-encapsulated antigen formulation.BIOMATERIALS,35(23),6086-6097.
MLA Zhang, Weifeng,et al."Immune responses to vaccines involving a combined antigen-nanoparticle mixture and nanoparticle-encapsulated antigen formulation".BIOMATERIALS 35.23(2014):6086-6097.
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