Knowledge Management System Of Institute of process engineering,CAS
| Stabilization of target protein during bioseparation | |
| Alternative Title | Prog. Biotechnol. |
| Feng, X. L.; Jin, Y. T.; Su, Z. G. | |
| 2000 | |
| Conference Name | International Conference on Bioseparation Engineering: Recovery and Recycle of Resources to Protect the Global Environment |
| Pages | 9-14 |
| Conference Date | JUL 04-07, 1999 |
| Conference Place | NIKKO, JAPAN |
| Publisher | Elsevier Science Bv |
| Abstract | Denaturation of target protein by various separation and purification steps contributes significant part to the total product loss in bioseparation. This report classifies the denaturation into four types including thermal denaturation, shear denaturation, solution denaturation and adsorption denaturation. For stabilization of target protein, three strategies are proposed including careful selection of unit operation to avoid detrimental action, process optimization to reduce the number of steps and the total processing time, and utilization of protective reagents such as PEG during bioseparation. It is important to understand the structure and property of the product to design the best bioseparation route. |
| Keyword | Polyethylene-glycol Chromatography |
| URL | 查看原文 |
| Indexed By | ISTP |
| Language | 英语 |
| Document Type | 会议论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/11097 |
| Collection | 研究所（批量导入） |
| Recommended Citation GB/T 7714 | Feng, X. L.,Jin, Y. T.,Su, Z. G.. Stabilization of target protein during bioseparation[C]:Elsevier Science Bv,2000:9-14. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Book-Bioseparation E（378KB） | 会议论文 | 限制开放 | CC BY-NC-SA | Application Full Text | ||
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