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Stabilization of target protein during bioseparation
Alternative TitleProg. Biotechnol.
Feng, X. L.; Jin, Y. T.; Su, Z. G.
Conference NameInternational Conference on Bioseparation Engineering: Recovery and Recycle of Resources to Protect the Global Environment
Conference DateJUL 04-07, 1999
Conference PlaceNIKKO, JAPAN
PublisherElsevier Science Bv
AbstractDenaturation of target protein by various separation and purification steps contributes significant part to the total product loss in bioseparation. This report classifies the denaturation into four types including thermal denaturation, shear denaturation, solution denaturation and adsorption denaturation. For stabilization of target protein, three strategies are proposed including careful selection of unit operation to avoid detrimental action, process optimization to reduce the number of steps and the total processing time, and utilization of protective reagents such as PEG during bioseparation. It is important to understand the structure and property of the product to design the best bioseparation route.
KeywordPolyethylene-glycol Chromatography
Indexed ByISTP
Document Type会议论文
Recommended Citation
GB/T 7714
Feng, X. L.,Jin, Y. T.,Su, Z. G.. Stabilization of target protein during bioseparation[C]:Elsevier Science Bv,2000:9-14.
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Book-Bioseparation E(378KB)会议论文 限制开放CC BY-NC-SAApplication Full Text
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