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Culture and characteristics of recombinant protein production of an Escherichia coli strain expressing carboxylesterase B1
Alternative TitleInt. Biodeterior. Biodegrad.
Qiao, C. L.; Shen, B. C.; Xing, J. M.; Huang, J.; Zhang, J. L.; Zhao, D. X.; Yang, B.
2006
Source PublicationInternational Biodeterioration & Biodegradation
Issue2
Pages77-81
AbstractHigh-density culture was achieved through controlling specific growth rate by limiting glucose concentration to < 0.2gL(-1). Carboxylesterase B I capable of hydrolyzing organophosphate esters was purified from Escherichia coli strain BL21 carrying a cloned esterase B I gene from mosquito. The recombinant strain BL21 carrying pET-ESTB1 was used for the fermentation. Product formation was induced by either a temperature shift from 30 to 42 degrees C or by feeding a mixture of glucose and lactose. Cell growth and production of detoxifying enzyme were affected by oxygen availability. The maximum biomass of E. coli BL21 (pET-ESTB1) increased from 14.9 to 31.5 g dry cell weight l(-1). Using the host strain E. coli BL21 (DE3), detoxifying enzyme was over-expressed at a biomass level of up to 31.5 g dry weight l(-1). The enzyme had a molecular mass of 64 kDa, its optimum temperature was approx. 37 degrees C; at pH 7 the relative activity after 3 h was 85.9% at 28 degrees C, 64.9% at 34 degrees C, and 4.5% at 40 degrees C. The enzyme activity of cells grown at lower temperatures was much higher; at 18 degrees C it almost twice than at 20 or 22 degrees C. degrees 2006 Elsevier Ltd. All rights reserved.
KeywordHigh-density Culture e Coli Carboxylesterase B1 Recombinant Gene Expression High-cell-density Fed-batch Cultures Growth Cultivation Pollutants Glucose
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Language英语
Document Type会议论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/11205
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
Qiao, C. L.,Shen, B. C.,Xing, J. M.,et al. Culture and characteristics of recombinant protein production of an Escherichia coli strain expressing carboxylesterase B1[C],2006:77-81.
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