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Inhibitory effect of vanillin on cellulase activity in hydrolysis of cellulosic biomass
Alternative TitleBioresour. Technol.
Li, Yun1,2; Qi, Benkun1; Wan, Yinhua1
2014-09-01
Source PublicationBIORESOURCE TECHNOLOGY
ISSN0960-8524
Volume167Issue:sepPages:324-330
AbstractPretreatment of lignocellulosic material produces a wide variety of inhibitory compounds, which strongly inhibit the following enzymatic hydrolysis of cellulosic biomass. Vanillin is a kind of phenolics derived from degradation of lignin. The effect of vanillin on cellulase activity for the hydrolysis of cellulose was investigated in detail. The results clearly showed that vanillin can reversibly and non-competitively inhibit the cellulase activity at appropriate concentrations and the value of IC50 was estimated to be 30 g/L. The inhibition kinetics of cellulase by vanillin was studied using HCH-1 model and inhibition constants were determined. Moreover, investigation of three compounds with similar structure of vanillin on cellulase activity demonstrated that aldehyde group and phenolic hydroxyl groups of vanillin had inhibitory effect on cellulase. These results provide valuable and detailed information for understanding the inhibition of lignin derived phenolics on cellulase. (C) 2014 Elsevier Ltd. All rights reserved.; Pretreatment of lignocellulosic material produces a wide variety of inhibitory compounds, which strongly inhibit the following enzymatic hydrolysis of cellulosic biomass. Vanillin is a kind of phenolics derived from degradation of lignin. The effect of vanillin on cellulase activity for the hydrolysis of cellulose was investigated in detail. The results clearly showed that vanillin can reversibly and non-competitively inhibit the cellulase activity at appropriate concentrations and the value of IC50 was estimated to be 30 g/L. The inhibition kinetics of cellulase by vanillin was studied using HCH-1 model and inhibition constants were determined. Moreover, investigation of three compounds with similar structure of vanillin on cellulase activity demonstrated that aldehyde group and phenolic hydroxyl groups of vanillin had inhibitory effect on cellulase. These results provide valuable and detailed information for understanding the inhibition of lignin derived phenolics on cellulase. (C) 2014 Elsevier Ltd. All rights reserved.
KeywordVanillin Cellulase Inhibition Kinetics Inhibitory Group
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.biortech.2014.06.035
URL查看原文
Indexed BySCI
Language英语
WOS KeywordENZYMATIC-HYDROLYSIS ; LIGNOCELLULOSIC BIOMASS ; SACCHAROMYCES-CEREVISIAE ; DEGRADATION-PRODUCTS ; PHENOLIC-COMPOUNDS ; HCH-1 MODEL ; LIGNIN ; PRETREATMENT ; DETOXIFICATION ; FERMENTATION
WOS Research AreaAgriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS SubjectAgricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS IDWOS:000340891500045
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/11456
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Li, Yun,Qi, Benkun,Wan, Yinhua. Inhibitory effect of vanillin on cellulase activity in hydrolysis of cellulosic biomass[J]. BIORESOURCE TECHNOLOGY,2014,167(sep):324-330.
APA Li, Yun,Qi, Benkun,&Wan, Yinhua.(2014).Inhibitory effect of vanillin on cellulase activity in hydrolysis of cellulosic biomass.BIORESOURCE TECHNOLOGY,167(sep),324-330.
MLA Li, Yun,et al."Inhibitory effect of vanillin on cellulase activity in hydrolysis of cellulosic biomass".BIORESOURCE TECHNOLOGY 167.sep(2014):324-330.
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