Knowledge Management System Of Institute of process engineering,CAS
| Inhibitory effect of vanillin on cellulase activity in hydrolysis of cellulosic biomass | |
| Alternative Title | Bioresour. Technol. |
| Li, Yun1,2; Qi, Benkun1; Wan, Yinhua1 | |
| 2014-09-01 | |
| Source Publication | BIORESOURCE TECHNOLOGY
 |
| ISSN | 0960-8524 |
| Volume | 167Issue:sepPages:324-330 |
| Abstract | Pretreatment of lignocellulosic material produces a wide variety of inhibitory compounds, which strongly inhibit the following enzymatic hydrolysis of cellulosic biomass. Vanillin is a kind of phenolics derived from degradation of lignin. The effect of vanillin on cellulase activity for the hydrolysis of cellulose was investigated in detail. The results clearly showed that vanillin can reversibly and non-competitively inhibit the cellulase activity at appropriate concentrations and the value of IC50 was estimated to be 30 g/L. The inhibition kinetics of cellulase by vanillin was studied using HCH-1 model and inhibition constants were determined. Moreover, investigation of three compounds with similar structure of vanillin on cellulase activity demonstrated that aldehyde group and phenolic hydroxyl groups of vanillin had inhibitory effect on cellulase. These results provide valuable and detailed information for understanding the inhibition of lignin derived phenolics on cellulase. (C) 2014 Elsevier Ltd. All rights reserved.; Pretreatment of lignocellulosic material produces a wide variety of inhibitory compounds, which strongly inhibit the following enzymatic hydrolysis of cellulosic biomass. Vanillin is a kind of phenolics derived from degradation of lignin. The effect of vanillin on cellulase activity for the hydrolysis of cellulose was investigated in detail. The results clearly showed that vanillin can reversibly and non-competitively inhibit the cellulase activity at appropriate concentrations and the value of IC50 was estimated to be 30 g/L. The inhibition kinetics of cellulase by vanillin was studied using HCH-1 model and inhibition constants were determined. Moreover, investigation of three compounds with similar structure of vanillin on cellulase activity demonstrated that aldehyde group and phenolic hydroxyl groups of vanillin had inhibitory effect on cellulase. These results provide valuable and detailed information for understanding the inhibition of lignin derived phenolics on cellulase. (C) 2014 Elsevier Ltd. All rights reserved. |
| Keyword | Vanillin Cellulase Inhibition Kinetics Inhibitory Group |
| Subtype | Article |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| DOI | 10.1016/j.biortech.2014.06.035 |
| URL | 查看原文 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | ENZYMATIC-HYDROLYSIS ; LIGNOCELLULOSIC BIOMASS ; SACCHAROMYCES-CEREVISIAE ; DEGRADATION-PRODUCTS ; PHENOLIC-COMPOUNDS ; HCH-1 MODEL ; LIGNIN ; PRETREATMENT ; DETOXIFICATION ; FERMENTATION |
| WOS Research Area | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| WOS Subject | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| WOS ID | WOS:000340891500045 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Version | 出版稿 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/11456 |
| Collection | 研究所（批量导入） |
| Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Li, Yun,Qi, Benkun,Wan, Yinhua. Inhibitory effect of vanillin on cellulase activity in hydrolysis of cellulosic biomass[J]. BIORESOURCE TECHNOLOGY,2014,167(sep):324-330. |
| APA | Li, Yun,Qi, Benkun,&Wan, Yinhua.(2014).Inhibitory effect of vanillin on cellulase activity in hydrolysis of cellulosic biomass.BIORESOURCE TECHNOLOGY,167(sep),324-330. |
| MLA | Li, Yun,et al."Inhibitory effect of vanillin on cellulase activity in hydrolysis of cellulosic biomass".BIORESOURCE TECHNOLOGY 167.sep(2014):324-330. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Inhibitory effect of（1163KB） | 限制开放 | CC BY-NC-SA | Application Full Text | |||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment