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Hydrophilic organic/salt-containing aqueous two-phase solvent system for counter-current chromatography: A novel technique for separation of polar compounds
Alternative TitleJ. Chromatogr. A
Liu, Dan1; Zou, Xiaowei1; Gao, Mingzhe1; Gu, Ming2; Xiao, Hongbin1,3
2014-08-22
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1356Issue:augPages:157-162
AbstractHydrophilic organic/salt-containing aqueous two-phase system composing of ethanol, water and ammonium sulfate for separation polar compounds was investigated on multilayer coil associated with J-type HSCCC devices. Compared to the classical polar solvent system based on 1-butanol-water or PEG1000-ammonium sulfate-water, the water content of upper phase in ethanol-ammonium sulfate-water systems was from 53.7% to 32.8% (wt%), closed to PEG1000-ammonium sulfate-water aqueous two-phase systems and higher than 1-butanol-water (22.0%, wt%). Therefore, the polarity of ethanol-ammonium sulfate-water is in the middle of 1-butanol-water and PEG-ammonium sulfate-water system, which is quite good for separating polar compounds like phenols, nucleosides and amino acids with low partition coefficient in 1-octanol-water system. The retention of stationary phase in four elution mode on type-J counter-current chromatography devices with multilayer coil column changed from 26% to 71%. Hydrodynamic trend possess both intermediate and hydrophilic solvent system property, which closely related to the composition of solvent system. The applicability of this system was demonstrated by successful separation of adenosine, uridine guanosine and cytidine. (C) 2014 Elsevier B.V. All rights reserved.; Hydrophilic organic/salt-containing aqueous two-phase system composing of ethanol, water and ammonium sulfate for separation polar compounds was investigated on multilayer coil associated with J-type HSCCC devices. Compared to the classical polar solvent system based on 1-butanol-water or PEG1000-ammonium sulfate-water, the water content of upper phase in ethanol-ammonium sulfate-water systems was from 53.7% to 32.8% (wt%), closed to PEG1000-ammonium sulfate-water aqueous two-phase systems and higher than 1-butanol-water (22.0%, wt%). Therefore, the polarity of ethanol-ammonium sulfate-water is in the middle of 1-butanol-water and PEG-ammonium sulfate-water system, which is quite good for separating polar compounds like phenols, nucleosides and amino acids with low partition coefficient in 1-octanol-water system. The retention of stationary phase in four elution mode on type-J counter-current chromatography devices with multilayer coil column changed from 26% to 71%. Hydrodynamic trend possess both intermediate and hydrophilic solvent system property, which closely related to the composition of solvent system. The applicability of this system was demonstrated by successful separation of adenosine, uridine guanosine and cytidine. (C) 2014 Elsevier B.V. All rights reserved.
KeywordHydrophilic Organic/salt-containing Aqueous Two-phase System High-speed Counter-current Chromatography Partition Coefficient Elution Mode
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.chroma.2014.06.050
URL查看原文
Indexed BySCI
Language英语
WOS KeywordSALVIA-MILTIORRHIZA BUNGE ; FERMENTATION BROTHS ; CHLOROGENIC ACID ; QUINIC ACID ; PURIFICATION ; EXTRACTION ; PHASE ; 1,3-PROPANEDIOL ; ANTIOXIDANTS ; DERIVATIVES
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000340302600016
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/11465
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China
3.Chinese Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Liu, Dan,Zou, Xiaowei,Gao, Mingzhe,et al. Hydrophilic organic/salt-containing aqueous two-phase solvent system for counter-current chromatography: A novel technique for separation of polar compounds[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1356(aug):157-162.
APA Liu, Dan,Zou, Xiaowei,Gao, Mingzhe,Gu, Ming,&Xiao, Hongbin.(2014).Hydrophilic organic/salt-containing aqueous two-phase solvent system for counter-current chromatography: A novel technique for separation of polar compounds.JOURNAL OF CHROMATOGRAPHY A,1356(aug),157-162.
MLA Liu, Dan,et al."Hydrophilic organic/salt-containing aqueous two-phase solvent system for counter-current chromatography: A novel technique for separation of polar compounds".JOURNAL OF CHROMATOGRAPHY A 1356.aug(2014):157-162.
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