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| Coating Gigaporous Polystyrene Microspheres with Cross-linked Poly(vinyl alcohol) Hydrogel as a Rapid Protein Chromatography Matrix | |
| Alternative Title | ACS Appl. Mater. Interfaces |
| Qu, Jian-Bo1; Huan, Guan-Sheng1; Chen, Yan-Li1; Zhou, Wei-Qing2; Liu, Jian-Guo1; Huang, Fang1 | |
| 2014-08-13 | |
| Source Publication | ACS APPLIED MATERIALS & INTERFACES
 |
| ISSN | 1944-8244 |
| Volume | 6Issue:15Pages:12752-12760 |
| Abstract | Gigaporous polystyrene (PS) microspheres were hydrophilized by in situ polymerization to give a stable cross-linked poly(vinyl alcohol) (PVA) hydrogel coating, which can shield proteins from the hydrophobic PS surface underneath. The amination of microspheres (PS-NH2) was first carried out through acetylization, oximation and reduction, and then 4,4'-azobis (4-cyanovaleric acid) (ACV), a polymerization initiator, was covalently immobilized on PS-NH2 through amide bond formation, and the cross-linked poly(vinyl acetate) (PVAc) was prepared by radical polymerization at the surfaces of ACV-immobilized PS microspheres (PS-ACV). Finally, the cross-linked PVA hydrogel coated gigaporous PS microspheres (PS-PVA) was easily achieved through alcoholysis of PVAc. Results suggested that the PS microspheres were effectively coated with cross-linked PVA hydrogel, where the gigaporrous structure remained under optimal conditions. After hydrophilic modification (PS-PVA), the protein-resistant ability of microspheres was greatly improved. The hydroxyl-rich PS-PVA surface can be easily derivatized by classical chemical methods. Performance advantages of the PS-PVA column in flow experiment include good permeability, low backpressure, and mechanical stability. These results indicated that PS-PVA should be promising in rapid protein chromatography.; Gigaporous polystyrene (PS) microspheres were hydrophilized by in situ polymerization to give a stable cross-linked poly(vinyl alcohol) (PVA) hydrogel coating, which can shield proteins from the hydrophobic PS surface underneath. The amination of microspheres (PS-NH2) was first carried out through acetylization, oximation and reduction, and then 4,4'-azobis (4-cyanovaleric acid) (ACV), a polymerization initiator, was covalently immobilized on PS-NH2 through amide bond formation, and the cross-linked poly(vinyl acetate) (PVAc) was prepared by radical polymerization at the surfaces of ACV-immobilized PS microspheres (PS-ACV). Finally, the cross-linked PVA hydrogel coated gigaporous PS microspheres (PS-PVA) was easily achieved through alcoholysis of PVAc. Results suggested that the PS microspheres were effectively coated with cross-linked PVA hydrogel, where the gigaporrous structure remained under optimal conditions. After hydrophilic modification (PS-PVA), the protein-resistant ability of microspheres was greatly improved. The hydroxyl-rich PS-PVA surface can be easily derivatized by classical chemical methods. Performance advantages of the PS-PVA column in flow experiment include good permeability, low backpressure, and mechanical stability. These results indicated that PS-PVA should be promising in rapid protein chromatography. |
| Keyword | Gigaporous Coating Polystyrene Particles Cross-linked Poly(Vinyl Alcohol) Hydrogel Protein Adsorption |
| Subtype | Article |
| WOS Headings | Science & Technology ; Technology |
| DOI | 10.1021/am5027067 |
| URL | 查看原文 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | PERFORMANCE LIQUID-CHROMATOGRAPHY ; PERFUSION CHROMATOGRAPHY ; AFFINITY-CHROMATOGRAPHY ; STATIONARY-PHASE ; ADSORPTION ; SEPARATION ; PARTICLES ; HYDROPHILIZATION ; SUPPORTS ; DEXTRAN |
| WOS Research Area | Science & Technology - Other Topics ; Materials Science |
| WOS Subject | Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
| WOS ID | WOS:000340446300112 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Version | 出版稿 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/11508 |
| Collection | 研究所（批量导入） |
| Affiliation | 1.China Univ Petr East China, Ctr Bioengn & Biotechnol, State Key Lab Heavy Oil Proc, Qingdao 266580, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| Recommended Citation GB/T 7714 | Qu, Jian-Bo,Huan, Guan-Sheng,Chen, Yan-Li,et al. Coating Gigaporous Polystyrene Microspheres with Cross-linked Poly(vinyl alcohol) Hydrogel as a Rapid Protein Chromatography Matrix[J]. ACS APPLIED MATERIALS & INTERFACES,2014,6(15):12752-12760. |
| APA | Qu, Jian-Bo,Huan, Guan-Sheng,Chen, Yan-Li,Zhou, Wei-Qing,Liu, Jian-Guo,&Huang, Fang.(2014).Coating Gigaporous Polystyrene Microspheres with Cross-linked Poly(vinyl alcohol) Hydrogel as a Rapid Protein Chromatography Matrix.ACS APPLIED MATERIALS & INTERFACES,6(15),12752-12760. |
| MLA | Qu, Jian-Bo,et al."Coating Gigaporous Polystyrene Microspheres with Cross-linked Poly(vinyl alcohol) Hydrogel as a Rapid Protein Chromatography Matrix".ACS APPLIED MATERIALS & INTERFACES 6.15(2014):12752-12760. |
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| File Name/Size | DocType | Version | Access | License | ||
| Coating Gigaporous P（6660KB） | 限制开放 | CC BY-NC-SA | Application Full Text | |||
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