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Coating Gigaporous Polystyrene Microspheres with Cross-linked Poly(vinyl alcohol) Hydrogel as a Rapid Protein Chromatography Matrix
Alternative TitleACS Appl. Mater. Interfaces
Qu, Jian-Bo1; Huan, Guan-Sheng1; Chen, Yan-Li1; Zhou, Wei-Qing2; Liu, Jian-Guo1; Huang, Fang1
2014-08-13
Source PublicationACS APPLIED MATERIALS & INTERFACES
ISSN1944-8244
Volume6Issue:15Pages:12752-12760
AbstractGigaporous polystyrene (PS) microspheres were hydrophilized by in situ polymerization to give a stable cross-linked poly(vinyl alcohol) (PVA) hydrogel coating, which can shield proteins from the hydrophobic PS surface underneath. The amination of microspheres (PS-NH2) was first carried out through acetylization, oximation and reduction, and then 4,4'-azobis (4-cyanovaleric acid) (ACV), a polymerization initiator, was covalently immobilized on PS-NH2 through amide bond formation, and the cross-linked poly(vinyl acetate) (PVAc) was prepared by radical polymerization at the surfaces of ACV-immobilized PS microspheres (PS-ACV). Finally, the cross-linked PVA hydrogel coated gigaporous PS microspheres (PS-PVA) was easily achieved through alcoholysis of PVAc. Results suggested that the PS microspheres were effectively coated with cross-linked PVA hydrogel, where the gigaporrous structure remained under optimal conditions. After hydrophilic modification (PS-PVA), the protein-resistant ability of microspheres was greatly improved. The hydroxyl-rich PS-PVA surface can be easily derivatized by classical chemical methods. Performance advantages of the PS-PVA column in flow experiment include good permeability, low backpressure, and mechanical stability. These results indicated that PS-PVA should be promising in rapid protein chromatography.; Gigaporous polystyrene (PS) microspheres were hydrophilized by in situ polymerization to give a stable cross-linked poly(vinyl alcohol) (PVA) hydrogel coating, which can shield proteins from the hydrophobic PS surface underneath. The amination of microspheres (PS-NH2) was first carried out through acetylization, oximation and reduction, and then 4,4'-azobis (4-cyanovaleric acid) (ACV), a polymerization initiator, was covalently immobilized on PS-NH2 through amide bond formation, and the cross-linked poly(vinyl acetate) (PVAc) was prepared by radical polymerization at the surfaces of ACV-immobilized PS microspheres (PS-ACV). Finally, the cross-linked PVA hydrogel coated gigaporous PS microspheres (PS-PVA) was easily achieved through alcoholysis of PVAc. Results suggested that the PS microspheres were effectively coated with cross-linked PVA hydrogel, where the gigaporrous structure remained under optimal conditions. After hydrophilic modification (PS-PVA), the protein-resistant ability of microspheres was greatly improved. The hydroxyl-rich PS-PVA surface can be easily derivatized by classical chemical methods. Performance advantages of the PS-PVA column in flow experiment include good permeability, low backpressure, and mechanical stability. These results indicated that PS-PVA should be promising in rapid protein chromatography.
KeywordGigaporous Coating Polystyrene Particles Cross-linked Poly(Vinyl Alcohol) Hydrogel Protein Adsorption
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
DOI10.1021/am5027067
URL查看原文
Indexed BySCI
Language英语
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; PERFUSION CHROMATOGRAPHY ; AFFINITY-CHROMATOGRAPHY ; STATIONARY-PHASE ; ADSORPTION ; SEPARATION ; PARTICLES ; HYDROPHILIZATION ; SUPPORTS ; DEXTRAN
WOS Research AreaScience & Technology - Other Topics ; Materials Science
WOS SubjectNanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS IDWOS:000340446300112
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/11508
Collection研究所(批量导入)
Affiliation1.China Univ Petr East China, Ctr Bioengn & Biotechnol, State Key Lab Heavy Oil Proc, Qingdao 266580, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Qu, Jian-Bo,Huan, Guan-Sheng,Chen, Yan-Li,et al. Coating Gigaporous Polystyrene Microspheres with Cross-linked Poly(vinyl alcohol) Hydrogel as a Rapid Protein Chromatography Matrix[J]. ACS APPLIED MATERIALS & INTERFACES,2014,6(15):12752-12760.
APA Qu, Jian-Bo,Huan, Guan-Sheng,Chen, Yan-Li,Zhou, Wei-Qing,Liu, Jian-Guo,&Huang, Fang.(2014).Coating Gigaporous Polystyrene Microspheres with Cross-linked Poly(vinyl alcohol) Hydrogel as a Rapid Protein Chromatography Matrix.ACS APPLIED MATERIALS & INTERFACES,6(15),12752-12760.
MLA Qu, Jian-Bo,et al."Coating Gigaporous Polystyrene Microspheres with Cross-linked Poly(vinyl alcohol) Hydrogel as a Rapid Protein Chromatography Matrix".ACS APPLIED MATERIALS & INTERFACES 6.15(2014):12752-12760.
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