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Konjac glucomannan microspheres for low-cost desalting of protein solution
Alternative TitleCarbohydr. Polym.
Xiong, Zhidong1,2; Zhou, Weiqing1; Sun, Lijing1; Li, Xiunan1; Zhao, Dawei1,2; Chen, Yi1,2; Li, Juan1; Ma, Guanghui1; Su, Zhiguo1
2014-10-13
Source PublicationCARBOHYDRATE POLYMERS
ISSN0144-8617
Volume111Issue:OCTPages:56-62
AbstractIn this study, low-cost konjac glucomannan (KGM) microspheres used for desalting were developed by an inverse dispersion method. High concentration of KGM was pretreated to reduce viscosity by acid hydrolysis method under the condition of high temperature and pressure. The selectivity of the obtained cross-linked KGM gels with different degree of crosslinking was studied by constructing calibration curves (K-av) of standard molecular weight substances. The stability of the gels was investigated, which showed that the KGM microspheres are tolerant to a wide range of pH and stable in all commonly used aqueous buffers, and insensitive to autoclaving as well. Furthermore, protein-desalting performances of GM-1250, a cross-linked KGM microsphere, were evaluated with two proteins, bovine serum albumin and filamentous hemagglutinin, which turned out that GM-1250 is comparable to the widely used commercial product - Sephadex G25 Fine. From economic considerations, KGM gel is a prospective alternative for dextran gels in protein desalting process. (C) 2014 Elsevier Ltd. All rights reserved.; In this study, low-cost konjac glucomannan (KGM) microspheres used for desalting were developed by an inverse dispersion method. High concentration of KGM was pretreated to reduce viscosity by acid hydrolysis method under the condition of high temperature and pressure. The selectivity of the obtained cross-linked KGM gels with different degree of crosslinking was studied by constructing calibration curves (K-av) of standard molecular weight substances. The stability of the gels was investigated, which showed that the KGM microspheres are tolerant to a wide range of pH and stable in all commonly used aqueous buffers, and insensitive to autoclaving as well. Furthermore, protein-desalting performances of GM-1250, a cross-linked KGM microsphere, were evaluated with two proteins, bovine serum albumin and filamentous hemagglutinin, which turned out that GM-1250 is comparable to the widely used commercial product - Sephadex G25 Fine. From economic considerations, KGM gel is a prospective alternative for dextran gels in protein desalting process. (C) 2014 Elsevier Ltd. All rights reserved.
KeywordKonjac Glucomannan Gel Filtration Chromatography Desalting
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1016/j.carbpol.2014.04.059
URL查看原文
Indexed BySCI
Language英语
WOS KeywordION-EXCHANGE CHROMATOGRAPHY ; GRAFTED AGAROSE MEDIA ; DRUG-DELIVERY ; ADSORPTION ; HYDROGELS ; DIALYSIS ; MANNAN
WOS Research AreaChemistry ; Polymer Science
WOS SubjectChemistry, Applied ; Chemistry, Organic ; Polymer Science
WOS IDWOS:000340302100008
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/11570
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Xiong, Zhidong,Zhou, Weiqing,Sun, Lijing,et al. Konjac glucomannan microspheres for low-cost desalting of protein solution[J]. CARBOHYDRATE POLYMERS,2014,111(OCT):56-62.
APA Xiong, Zhidong.,Zhou, Weiqing.,Sun, Lijing.,Li, Xiunan.,Zhao, Dawei.,...&Su, Zhiguo.(2014).Konjac glucomannan microspheres for low-cost desalting of protein solution.CARBOHYDRATE POLYMERS,111(OCT),56-62.
MLA Xiong, Zhidong,et al."Konjac glucomannan microspheres for low-cost desalting of protein solution".CARBOHYDRATE POLYMERS 111.OCT(2014):56-62.
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