CAS OpenIR  > 研究所(批量导入)
Effects of cryopreservation on human mesenchymal stem cells attached to different substrates
Alternative TitleJ. Tissue Eng. Regen. Med.
Xu, Xia1,2; Liu, Yang2,3,4; Cui, Zhan Feng2
2014-08-01
Source PublicationJOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
ISSN1932-6254
Volume8Issue:8Pages:664-672
AbstractThere is a need to preserve cell-seeded scaffolds or cell-matrix constructs for tissue-engineering and other applications. Cryopreservation is likely to be the most practical method. The aim of this study was to investigate how cryopreservation affects cells attached to different substrates and how they respond differently from those in suspension. Human mesenchymal stem cells (hMSCs) were studied for their close relevance to tissue-engineering and stem cell therapy applications, in particular how cryopreservation affects cell adherence, cell growth and the viability of hMSCs attached to different substrates, including glass, gelatin, matrigel and a matrigel sandwich. The effects of cryopreservation on F-actin organization, intracellular pH and mitochondrial localization of the adherent hMSCs were further investigated. It was found that cells attached to a glass surface could hardly survive the common cryopreservation protocol using 10% DMSO and a 1 degrees C/min cooling rate. By contrast, cells attached to gelatin and matrigel could survive to a greater extent. Furthermore, cryopreservation affected the potential of cell attachment and proliferation, resulted in distortion of F-actin, led to alteration of intracellular pH of the hMSCs for all tested substrates and caused a change in the mitochondrial localization of hMSCs on a matrigel substrate and in a matrigel sandwich. Our results showed that cell attachment and cell viability could be improved by changing the interaction between cell and substrate through modification of the substrate properties, which has implications for scaffold design if cell-seeded scaffolds or engineered tissues need to be cryopreserved. Copyright (C) 2012 John Wiley & Sons, Ltd.; There is a need to preserve cell-seeded scaffolds or cell-matrix constructs for tissue-engineering and other applications. Cryopreservation is likely to be the most practical method. The aim of this study was to investigate how cryopreservation affects cells attached to different substrates and how they respond differently from those in suspension. Human mesenchymal stem cells (hMSCs) were studied for their close relevance to tissue-engineering and stem cell therapy applications, in particular how cryopreservation affects cell adherence, cell growth and the viability of hMSCs attached to different substrates, including glass, gelatin, matrigel and a matrigel sandwich. The effects of cryopreservation on F-actin organization, intracellular pH and mitochondrial localization of the adherent hMSCs were further investigated. It was found that cells attached to a glass surface could hardly survive the common cryopreservation protocol using 10% DMSO and a 1 degrees C/min cooling rate. By contrast, cells attached to gelatin and matrigel could survive to a greater extent. Furthermore, cryopreservation affected the potential of cell attachment and proliferation, resulted in distortion of F-actin, led to alteration of intracellular pH of the hMSCs for all tested substrates and caused a change in the mitochondrial localization of hMSCs on a matrigel substrate and in a matrigel sandwich. Our results showed that cell attachment and cell viability could be improved by changing the interaction between cell and substrate through modification of the substrate properties, which has implications for scaffold design if cell-seeded scaffolds or engineered tissues need to be cryopreserved. Copyright (C) 2012 John Wiley & Sons, Ltd.
KeywordHuman Mesenchymal Stem Cells Tissue Engineering Cell Attachment Cryopreservation Cell Recovery Scaffold Design
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1002/term.1570
URL查看原文
Indexed BySCI
Language英语
WOS KeywordBONE-MARROW ; IN-VITRO ; CULTURE ; MATRIX ; PROLIFERATION ; HEPATOCYTES ; PROGENITORS ; MONOLAYERS ; REMOVAL ; COMPLEX
WOS Research AreaCell Biology ; Biotechnology & Applied Microbiology ; Engineering
WOS SubjectCell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Cell Biology ; Engineering, Biomedical
WOS IDWOS:000340245900009
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/11578
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China
2.Univ Oxford, Dept Engn Sci, Inst Biomed Engn, Oxford OX1 3PJ, England
3.Dalian Univ Technol, Sch Chem Engn, Dalian R&D Ctr Stem Cell & Tissue Engn, Dalian, Peoples R China
4.Dalian Med Univ, Regenerat Med Ctr, Affiliated Hosp 1, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Xu, Xia,Liu, Yang,Cui, Zhan Feng. Effects of cryopreservation on human mesenchymal stem cells attached to different substrates[J]. JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE,2014,8(8):664-672.
APA Xu, Xia,Liu, Yang,&Cui, Zhan Feng.(2014).Effects of cryopreservation on human mesenchymal stem cells attached to different substrates.JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE,8(8),664-672.
MLA Xu, Xia,et al."Effects of cryopreservation on human mesenchymal stem cells attached to different substrates".JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE 8.8(2014):664-672.
Files in This Item:
File Name/Size DocType Version Access License
Effects of cryoprese(421KB) 限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Xu, Xia]'s Articles
[Liu, Yang]'s Articles
[Cui, Zhan Feng]'s Articles
Baidu academic
Similar articles in Baidu academic
[Xu, Xia]'s Articles
[Liu, Yang]'s Articles
[Cui, Zhan Feng]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Xu, Xia]'s Articles
[Liu, Yang]'s Articles
[Cui, Zhan Feng]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.