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Mechanistic studies for monodisperse exenatide-loaded PLGA microspheres prepared by different methods based on SPG membrane emulsification
Alternative TitleActa Biomater.
Qi, Feng1,2; Wu, Jie1; Yang, Tingyuan1; Ma, Guanghui1,3; Su, Zhiguo1,3
2014-10-01
Source PublicationACTA BIOMATERIALIA
ISSN1742-7061
Volume10Issue:10Pages:4247-4256
AbstractPoly(DL-lactic-co-glycolic acid) (PLGA) microspheres have been widely prepared by many methods, including solvent evaporation, solvent extraction and the co-solvent method. However, very few studies have compared the properties of microspheres fabricated by these methods. This is partly because the broad size distribution of the resultant particles severely complicates the analysis and affects the reliability of the comparison. To this end, uniform-sized PLGA microspheres have been prepared by Shirasu porous glass premix membrane emulsification and used to encapsulate exenatide, a drug for treating Type 2 diabetes. Based on this technique, the influences on the properties of microspheres fabricated by the aforementioned three methods were intensively investigated, including in vitro release, degradation and pharmacology. We found that these microspheres presented totally different release behaviors in vitro and in vivo, but exhibited a similar trend of PLGA degradation. Moreover, the internal structural evolution visually demonstrated these release behaviors. We selected for further examination the microsphere prepared by solvent evaporation because of its constant release rate, and explored its pharmacodynamics, histology, etc., in more detail. This microsphere when injected once showed equivalent efficacy to that of twice-daily injections of exenatide with no inflammatory response. (C) 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.; Poly(DL-lactic-co-glycolic acid) (PLGA) microspheres have been widely prepared by many methods, including solvent evaporation, solvent extraction and the co-solvent method. However, very few studies have compared the properties of microspheres fabricated by these methods. This is partly because the broad size distribution of the resultant particles severely complicates the analysis and affects the reliability of the comparison. To this end, uniform-sized PLGA microspheres have been prepared by Shirasu porous glass premix membrane emulsification and used to encapsulate exenatide, a drug for treating Type 2 diabetes. Based on this technique, the influences on the properties of microspheres fabricated by the aforementioned three methods were intensively investigated, including in vitro release, degradation and pharmacology. We found that these microspheres presented totally different release behaviors in vitro and in vivo, but exhibited a similar trend of PLGA degradation. Moreover, the internal structural evolution visually demonstrated these release behaviors. We selected for further examination the microsphere prepared by solvent evaporation because of its constant release rate, and explored its pharmacodynamics, histology, etc., in more detail. This microsphere when injected once showed equivalent efficacy to that of twice-daily injections of exenatide with no inflammatory response. (C) 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
KeywordPlga Controlled Release Exenatide Spg Membrane Emulsification Microsphere
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
DOI10.1016/j.actbio.2014.06.018
URL查看原文
Indexed BySCI
Language英语
WOS KeywordPOLY(LACTIC-CO-GLYCOLIC ACID) MICROSPHERES ; IN-VITRO ; BIODEGRADABLE MICROPARTICLES ; SUSTAINED-RELEASE ; PLA NANOPARTICLES ; PROTEIN DELIVERY ; DRUG-RELEASE ; MORPHOLOGY ; DEGRADATION ; EMULSION
WOS Research AreaEngineering ; Materials Science
WOS SubjectEngineering, Biomedical ; Materials Science, Biomaterials
WOS IDWOS:000342523800019
Citation statistics
Cited Times:38[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/11652
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, State Key Lab Biochem Engn, PLA Key Lab Biopharmaceut Prod & Formulat Engn, Inst Proc Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300072, Peoples R China
Recommended Citation
GB/T 7714
Qi, Feng,Wu, Jie,Yang, Tingyuan,et al. Mechanistic studies for monodisperse exenatide-loaded PLGA microspheres prepared by different methods based on SPG membrane emulsification[J]. ACTA BIOMATERIALIA,2014,10(10):4247-4256.
APA Qi, Feng,Wu, Jie,Yang, Tingyuan,Ma, Guanghui,&Su, Zhiguo.(2014).Mechanistic studies for monodisperse exenatide-loaded PLGA microspheres prepared by different methods based on SPG membrane emulsification.ACTA BIOMATERIALIA,10(10),4247-4256.
MLA Qi, Feng,et al."Mechanistic studies for monodisperse exenatide-loaded PLGA microspheres prepared by different methods based on SPG membrane emulsification".ACTA BIOMATERIALIA 10.10(2014):4247-4256.
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