CAS OpenIR  > 研究所(批量导入)
Characterisation of a chitinase from Pseudoalteromonas sp. DL-6, a marine psychrophilic bacterium
Alternative TitleInt. J. Biol. Macromol.
Wang, Xiaohui1,2,3,5; Zhao, Yong1; Tan, Haidong1; Chi, Naiyu2; Zhang, Qingfang2; Du, Yuguang4; Yin, Heng1
2014-09-01
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
Volume70Issue:SEP.Pages:455-462
AbstractIn this study, we isolated a new psychrophilic bacterium, Pseudoalteromonas sp. DL-6 from marine sediments, which grew well on chitin-containing plates at 4 degrees C. One endo-type chitinase gene, chiA, was cloned from the genomic DNA of this bacterium and heterologously expressed in Escherichia coli BL21 (DE3). ChiA showed very high catalytic activity, even at 4 degrees C, and exhibited maximal activity on a chitinous substrate at pH 8.0 and 20 degrees C. Kinetic studies indicated that ChiA has a greater catalytic efficiency on 4-methylumbelliferyl-beta-D-N,N',N"-triacetylchitotriose[4-MU(GlcNAc)(3)] than on 4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside[4-MU(GlcNAc)(2)]. Electrospray ionisation mass spectrometry (ESI-MS) analysis showed that the hydrolysis products of powdered chitin after ChiA digestion consisted of a series of chitin oligomers with different degrees of polymerisation. The ChiA mode of action was also examined using (GlcNAc)(2-6) as a substrate, and the results suggested that ChiA is a non-processive endo-type chitinase. (C) 2014 Elsevier B.V. All rights reserved.; In this study, we isolated a new psychrophilic bacterium, Pseudoalteromonas sp. DL-6 from marine sediments, which grew well on chitin-containing plates at 4 degrees C. One endo-type chitinase gene, chiA, was cloned from the genomic DNA of this bacterium and heterologously expressed in Escherichia coli BL21 (DE3). ChiA showed very high catalytic activity, even at 4 degrees C, and exhibited maximal activity on a chitinous substrate at pH 8.0 and 20 degrees C. Kinetic studies indicated that ChiA has a greater catalytic efficiency on 4-methylumbelliferyl-beta-D-N,N',N"-triacetylchitotriose[4-MU(GlcNAc)(3)] than on 4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside[4-MU(GlcNAc)(2)]. Electrospray ionisation mass spectrometry (ESI-MS) analysis showed that the hydrolysis products of powdered chitin after ChiA digestion consisted of a series of chitin oligomers with different degrees of polymerisation. The ChiA mode of action was also examined using (GlcNAc)(2-6) as a substrate, and the results suggested that ChiA is a non-processive endo-type chitinase. (C) 2014 Elsevier B.V. All rights reserved.
KeywordChitinase Purification Characterisation
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.ijbiomac.2014.07.033
URL查看原文
Indexed BySCI
Language英语
WOS KeywordSP STRAIN O-7 ; SERRATIA-MARCESCENS ; RECALCITRANT POLYSACCHARIDES ; FAMILY-18 CHITINASE ; MOLECULAR ANALYSIS ; MORITELLA-MARINA ; BINDING DOMAIN ; GENE ; PROCESSIVITY ; DEGRADATION
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000342478800065
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/11658
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Univ, Sch Life Sci & Biotechnol, Dalian 116622, Peoples R China
3.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China
4.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wang, Xiaohui,Zhao, Yong,Tan, Haidong,et al. Characterisation of a chitinase from Pseudoalteromonas sp. DL-6, a marine psychrophilic bacterium[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2014,70(SEP.):455-462.
APA Wang, Xiaohui.,Zhao, Yong.,Tan, Haidong.,Chi, Naiyu.,Zhang, Qingfang.,...&Yin, Heng.(2014).Characterisation of a chitinase from Pseudoalteromonas sp. DL-6, a marine psychrophilic bacterium.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,70(SEP.),455-462.
MLA Wang, Xiaohui,et al."Characterisation of a chitinase from Pseudoalteromonas sp. DL-6, a marine psychrophilic bacterium".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 70.SEP.(2014):455-462.
Files in This Item:
File Name/Size DocType Version Access License
Characterisation of (904KB) 限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Xiaohui]'s Articles
[Zhao, Yong]'s Articles
[Tan, Haidong]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Xiaohui]'s Articles
[Zhao, Yong]'s Articles
[Tan, Haidong]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Xiaohui]'s Articles
[Zhao, Yong]'s Articles
[Tan, Haidong]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.