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Immunological detection of hepatocellular carcinoma biomarker GP73 based on dissolved magnetic nanoparticles
Alternative TitleColloid Surf. A-Physicochem. Eng. Asp.
Yang, Mingzhu1; Guan, Yueping1; Yang, Yu2; Xie, Li3; Xia, Tingting1; Xiong, Wubin1; Guo, Chen4
2014-02-20
Source PublicationCOLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
ISSN0927-7757
Volume443Issue:FEB.Pages:280-285
AbstractThe peroxidase-like activity of dissolved Fe3O4 magnetic nanoparticles was reported in this work. The chromogenic results of dissolved magnetic nanoparticles demonstrated almost the same change trend as standard ferric ion solutions. And the chromogenic reactions of dissolved Fe3O4 magnetic nanoparticles were more sensitive than that of whole Fe3O4 magnetic nanoparticles. The chromogenic reaction of dissolved Fe3O4 magnetic nanoparticles was utilized to replace the chromogenic reaction of horseradish peroxidase in traditional enzyme-linked immunosorbent assay and applied in the immunoassay of hepatocellular carcinoma biomarker GP73. The method based on the chromogenic reaction of dissolved Fe3O4 magnetic nanoparticles achieved a detection limit of 250 ng/mL, which was more sensitive than the method based on the peroxidase-like activity of whole Fe3O4 magnetic nanoparticles. The immunological detection method based on dissolved Fe3O4 magnetic nanoparticles proved to be simple, sensitive, specific and low-cost in this paper. (C) 2013 Elsevier B.V. All rights reserved.; The peroxidase-like activity of dissolved Fe3O4 magnetic nanoparticles was reported in this work. The chromogenic results of dissolved magnetic nanoparticles demonstrated almost the same change trend as standard ferric ion solutions. And the chromogenic reactions of dissolved Fe3O4 magnetic nanoparticles were more sensitive than that of whole Fe3O4 magnetic nanoparticles. The chromogenic reaction of dissolved Fe3O4 magnetic nanoparticles was utilized to replace the chromogenic reaction of horseradish peroxidase in traditional enzyme-linked immunosorbent assay and applied in the immunoassay of hepatocellular carcinoma biomarker GP73. The method based on the chromogenic reaction of dissolved Fe3O4 magnetic nanoparticles achieved a detection limit of 250 ng/mL, which was more sensitive than the method based on the peroxidase-like activity of whole Fe3O4 magnetic nanoparticles. The immunological detection method based on dissolved Fe3O4 magnetic nanoparticles proved to be simple, sensitive, specific and low-cost in this paper. (C) 2013 Elsevier B.V. All rights reserved.
KeywordMagnetic Nanoparticles Gp73 Immunoassay
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1016/j.colsurfa.2013.11.026
URL查看原文
Indexed BySCI
Language英语
WOS KeywordPEROXIDASE-LIKE ACTIVITY ; SENSITIVE DETECTION ; HYDROGEN-PEROXIDE ; IMMUNOASSAY ; ASSAY ; SERUM
WOS Research AreaChemistry
WOS SubjectChemistry, Physical
WOS IDWOS:000342501300039
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/11661
Collection研究所(批量导入)
Affiliation1.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China
2.China Acad Inspect & Quarantine, Beijing 100123, Peoples R China
3.Capital Med Univ, Beijing Youan Hosp, Beijing 100054, Peoples R China
4.Chinese Acad Sci, Inst Proc Engn, Lab Separat Sci & Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Yang, Mingzhu,Guan, Yueping,Yang, Yu,et al. Immunological detection of hepatocellular carcinoma biomarker GP73 based on dissolved magnetic nanoparticles[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2014,443(FEB.):280-285.
APA Yang, Mingzhu.,Guan, Yueping.,Yang, Yu.,Xie, Li.,Xia, Tingting.,...&Guo, Chen.(2014).Immunological detection of hepatocellular carcinoma biomarker GP73 based on dissolved magnetic nanoparticles.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,443(FEB.),280-285.
MLA Yang, Mingzhu,et al."Immunological detection of hepatocellular carcinoma biomarker GP73 based on dissolved magnetic nanoparticles".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 443.FEB.(2014):280-285.
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