Knowledge Management System Of Institute of process engineering,CAS
| Immunological detection of hepatocellular carcinoma biomarker GP73 based on dissolved magnetic nanoparticles | |
| Alternative Title | Colloid Surf. A-Physicochem. Eng. Asp. |
| Yang, Mingzhu1; Guan, Yueping1; Yang, Yu2; Xie, Li3; Xia, Tingting1; Xiong, Wubin1; Guo, Chen4 | |
| 2014-02-20 | |
| Source Publication | COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
 |
| ISSN | 0927-7757 |
| Volume | 443Issue:FEB.Pages:280-285 |
| Abstract | The peroxidase-like activity of dissolved Fe3O4 magnetic nanoparticles was reported in this work. The chromogenic results of dissolved magnetic nanoparticles demonstrated almost the same change trend as standard ferric ion solutions. And the chromogenic reactions of dissolved Fe3O4 magnetic nanoparticles were more sensitive than that of whole Fe3O4 magnetic nanoparticles. The chromogenic reaction of dissolved Fe3O4 magnetic nanoparticles was utilized to replace the chromogenic reaction of horseradish peroxidase in traditional enzyme-linked immunosorbent assay and applied in the immunoassay of hepatocellular carcinoma biomarker GP73. The method based on the chromogenic reaction of dissolved Fe3O4 magnetic nanoparticles achieved a detection limit of 250 ng/mL, which was more sensitive than the method based on the peroxidase-like activity of whole Fe3O4 magnetic nanoparticles. The immunological detection method based on dissolved Fe3O4 magnetic nanoparticles proved to be simple, sensitive, specific and low-cost in this paper. (C) 2013 Elsevier B.V. All rights reserved.; The peroxidase-like activity of dissolved Fe3O4 magnetic nanoparticles was reported in this work. The chromogenic results of dissolved magnetic nanoparticles demonstrated almost the same change trend as standard ferric ion solutions. And the chromogenic reactions of dissolved Fe3O4 magnetic nanoparticles were more sensitive than that of whole Fe3O4 magnetic nanoparticles. The chromogenic reaction of dissolved Fe3O4 magnetic nanoparticles was utilized to replace the chromogenic reaction of horseradish peroxidase in traditional enzyme-linked immunosorbent assay and applied in the immunoassay of hepatocellular carcinoma biomarker GP73. The method based on the chromogenic reaction of dissolved Fe3O4 magnetic nanoparticles achieved a detection limit of 250 ng/mL, which was more sensitive than the method based on the peroxidase-like activity of whole Fe3O4 magnetic nanoparticles. The immunological detection method based on dissolved Fe3O4 magnetic nanoparticles proved to be simple, sensitive, specific and low-cost in this paper. (C) 2013 Elsevier B.V. All rights reserved. |
| Keyword | Magnetic Nanoparticles Gp73 Immunoassay |
| Subtype | Article |
| WOS Headings | Science & Technology ; Physical Sciences |
| DOI | 10.1016/j.colsurfa.2013.11.026 |
| URL | 查看原文 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | PEROXIDASE-LIKE ACTIVITY ; SENSITIVE DETECTION ; HYDROGEN-PEROXIDE ; IMMUNOASSAY ; ASSAY ; SERUM |
| WOS Research Area | Chemistry |
| WOS Subject | Chemistry, Physical |
| WOS ID | WOS:000342501300039 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Version | 出版稿 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/11661 |
| Collection | 研究所（批量导入） |
| Affiliation | 1.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China 2.China Acad Inspect & Quarantine, Beijing 100123, Peoples R China 3.Capital Med Univ, Beijing Youan Hosp, Beijing 100054, Peoples R China 4.Chinese Acad Sci, Inst Proc Engn, Lab Separat Sci & Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| Recommended Citation GB/T 7714 | Yang, Mingzhu,Guan, Yueping,Yang, Yu,et al. Immunological detection of hepatocellular carcinoma biomarker GP73 based on dissolved magnetic nanoparticles[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2014,443(FEB.):280-285. |
| APA | Yang, Mingzhu.,Guan, Yueping.,Yang, Yu.,Xie, Li.,Xia, Tingting.,...&Guo, Chen.(2014).Immunological detection of hepatocellular carcinoma biomarker GP73 based on dissolved magnetic nanoparticles.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,443(FEB.),280-285. |
| MLA | Yang, Mingzhu,et al."Immunological detection of hepatocellular carcinoma biomarker GP73 based on dissolved magnetic nanoparticles".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 443.FEB.(2014):280-285. |
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| Immunological detect（1557KB） | 限制开放 | CC BY-NC-SA | Application Full Text | |||
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