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Investigation of continuous-batch mode of two-stage culture of Nannochloropsis sp for lipid production
Alternative TitleBioprocess. Biosyst. Eng.
Zhang, Dongmei1,2; Xue, Shengzhang1; Sun, Zhongliang1; Liang, Kehong1; Wang, Lu3; Zhang, Qinghua1; Cong, Wei1
2014-10-01
Source PublicationBIOPROCESS AND BIOSYSTEMS ENGINEERING
ISSN1615-7591
Volume37Issue:10Pages:2073-2082
AbstractMicroalgal lipid induction through nitrogen stress often suffers from a contradiction between biomass productivity and lipid content, i.e., either high biomass productivity with low lipid content or vice versa. A two-stage nitrogen-replete and nitrogen-deplete (NR-ND) culture was suggested to be an option to attain high lipid productivity. In this study, the effects of culture conditions and modes on biomass productivity and lipid productivity of Nannochloropsis sp. in the two stages were comprehensively investigated. The optimal culture conditions for the two stages, aiming to high biomass productivity and lipid productivity respectively, were consistent, i.e., CO2 content in aeration (1 %), phosphorus concentration in medium (181 mu mol/L), incident light intensity (150 mu E/(m(2)s)), temperature (25 A degrees C). Different culture modes of the two stages were compared. The overall lipid productivity of the two-stage continuous-batch mode achieved 0.123 g/(L day), which was 60.3, 48.2, 34.9 and 13.5 % higher than that of single nitrogen-replete batch, single nitrogen-limited batch, continuous nitrogen-replete culture and two-stage batch-batch culture, respectively, and also higher than most reported values. This contribution provides fundamental data for the two-stage NR-ND cultivation process design of Nannochloropsis sp.; Microalgal lipid induction through nitrogen stress often suffers from a contradiction between biomass productivity and lipid content, i.e., either high biomass productivity with low lipid content or vice versa. A two-stage nitrogen-replete and nitrogen-deplete (NR-ND) culture was suggested to be an option to attain high lipid productivity. In this study, the effects of culture conditions and modes on biomass productivity and lipid productivity of Nannochloropsis sp. in the two stages were comprehensively investigated. The optimal culture conditions for the two stages, aiming to high biomass productivity and lipid productivity respectively, were consistent, i.e., CO2 content in aeration (1 %), phosphorus concentration in medium (181 mu mol/L), incident light intensity (150 mu E/(m(2)s)), temperature (25 A degrees C). Different culture modes of the two stages were compared. The overall lipid productivity of the two-stage continuous-batch mode achieved 0.123 g/(L day), which was 60.3, 48.2, 34.9 and 13.5 % higher than that of single nitrogen-replete batch, single nitrogen-limited batch, continuous nitrogen-replete culture and two-stage batch-batch culture, respectively, and also higher than most reported values. This contribution provides fundamental data for the two-stage NR-ND cultivation process design of Nannochloropsis sp.
KeywordLipid Productivity Two-stage Cultivation Nannochloropsis Sp. Continuous Culture Medium Recycle
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1007/s00449-014-1185-6
URL查看原文
Indexed BySCI
Language英语
WOS KeywordFATTY-ACID-COMPOSITION ; CHLORELLA-VULGARIS ; CHLAMYDOMONAS-REINHARDTII ; BIODIESEL PRODUCTION ; BIOFUELS PRODUCTION ; WASTE-WATER ; MICROALGAE ; GROWTH ; ACCUMULATION ; TEMPERATURE
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
WOS IDWOS:000342171500017
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/11666
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing Key Lab Bioproc, Beijing 100029, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Dongmei,Xue, Shengzhang,Sun, Zhongliang,et al. Investigation of continuous-batch mode of two-stage culture of Nannochloropsis sp for lipid production[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2014,37(10):2073-2082.
APA Zhang, Dongmei.,Xue, Shengzhang.,Sun, Zhongliang.,Liang, Kehong.,Wang, Lu.,...&Cong, Wei.(2014).Investigation of continuous-batch mode of two-stage culture of Nannochloropsis sp for lipid production.BIOPROCESS AND BIOSYSTEMS ENGINEERING,37(10),2073-2082.
MLA Zhang, Dongmei,et al."Investigation of continuous-batch mode of two-stage culture of Nannochloropsis sp for lipid production".BIOPROCESS AND BIOSYSTEMS ENGINEERING 37.10(2014):2073-2082.
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