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Simple Technique for Preparing Stable and Recyclable Cross-Linked Enzyme Aggregates with Crude-Pored Microspherical Silica Core
Alternative TitleInd. Eng. Chem. Res.
Cui, Jian Dong1,2,3; Li, Lian Lian1; Zhao, Ya Min1
2014-10-22
Source PublicationINDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
ISSN0888-5885
Volume53Issue:42Pages:16176-16182
AbstractWith the aim of providing a highly stable and easy recyclable biocatalyst, we prepared a new biocatalyst formulation based on cross-linked enzyme aggregate (CLEA) technology by the physical adsorption of cross-linked phenylalanine ammonia lyase on a highly porous microspherical silica gel solid support (S-CLEAs). The main factors in the preparation of S-CLEAs were optimized by response surface methodology (RSM). Analysis by scanning electron microscopy (SEM), FT-IR spectroscopy, and confocal laser scanning microscopy (CLSM) indicated that the CLEAs were successfully immobilized on the crude-pored microspherical silica gel. Compared to standard CLEAs, the S-CLEAs can be easily separated from the reaction mixture by natural precipitation without centrifugation and filtration treatments. Although the S-CLEAs revealed an excellent stability of the enzyme against high temperature and denaturants, the volumetric activity of the S-CLEAs decreased because of the presence of silica gel. In a word, the S-CLEA technology in this study might open an attractive route toward effectively improving the catalytic properties of traditional CLEAs.; With the aim of providing a highly stable and easy recyclable biocatalyst, we prepared a new biocatalyst formulation based on cross-linked enzyme aggregate (CLEA) technology by the physical adsorption of cross-linked phenylalanine ammonia lyase on a highly porous microspherical silica gel solid support (S-CLEAs). The main factors in the preparation of S-CLEAs were optimized by response surface methodology (RSM). Analysis by scanning electron microscopy (SEM), FT-IR spectroscopy, and confocal laser scanning microscopy (CLSM) indicated that the CLEAs were successfully immobilized on the crude-pored microspherical silica gel. Compared to standard CLEAs, the S-CLEAs can be easily separated from the reaction mixture by natural precipitation without centrifugation and filtration treatments. Although the S-CLEAs revealed an excellent stability of the enzyme against high temperature and denaturants, the volumetric activity of the S-CLEAs decreased because of the presence of silica gel. In a word, the S-CLEA technology in this study might open an attractive route toward effectively improving the catalytic properties of traditional CLEAs.
KeywordPhenylalanine Ammonia-lyase Immobilization Stabilization Phenylketonuria Cleas Microcapsules Biocatalysts Replacement Strategies Stability
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
DOI10.1021/ie5021206
URL查看原文
Indexed BySCI
Language英语
WOS KeywordPHENYLALANINE AMMONIA-LYASE ; IMMOBILIZATION ; STABILIZATION ; PHENYLKETONURIA ; CLEAS ; MICROCAPSULES ; BIOCATALYSTS ; REPLACEMENT ; STRATEGIES ; STABILITY
WOS Research AreaEngineering
WOS SubjectEngineering, Chemical
WOS IDWOS:000343687400008
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/11668
Collection研究所(批量导入)
Affiliation1.Hebei Univ Sci & Technol, Coll Biosci & Bioengn, Res Ctr Fermentat Engn Hebei, Shijiazhuang 050000, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China
3.Tianjin Univ Sci & Technol, Key Lab Ind Fermentat Microbiol, Minist Educ, Tianjin 300457, Peoples R China
Recommended Citation
GB/T 7714
Cui, Jian Dong,Li, Lian Lian,Zhao, Ya Min. Simple Technique for Preparing Stable and Recyclable Cross-Linked Enzyme Aggregates with Crude-Pored Microspherical Silica Core[J]. INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,2014,53(42):16176-16182.
APA Cui, Jian Dong,Li, Lian Lian,&Zhao, Ya Min.(2014).Simple Technique for Preparing Stable and Recyclable Cross-Linked Enzyme Aggregates with Crude-Pored Microspherical Silica Core.INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,53(42),16176-16182.
MLA Cui, Jian Dong,et al."Simple Technique for Preparing Stable and Recyclable Cross-Linked Enzyme Aggregates with Crude-Pored Microspherical Silica Core".INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH 53.42(2014):16176-16182.
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