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Biodegradable polylactide microspheres enhance specific immune response induced by Hepatitis B surface antigen
Alternative TitleHuman Vaccines Immunother.
Qiu, Shaohui1; Wei, Qiang2; Liang, Zhenglun1; Ma, Guanghui2; Wang, Lianyan2; An, Wenqi3; Ma, Xiaowei3; Fang, Xin1; He, Peng1; Li, Hemin1; Hu, Zhongyu1
2014
Source PublicationHUMAN VACCINES & IMMUNOTHERAPEUTICS
ISSN2164-5515
Volume10Issue:8Pages:2350-2356
AbstractHepatitis B (HB) infection caused by Hepatitis B virus (HBV) is the most common liver disease in the world. HB vaccine, when administered in conjunction with alum adjuvants, induces Th2 immunity that confers protection against HBV. However, currently available vaccine formulations and adjuvants do not elicit adequate Th1 and CTL responses that are important for prevention of maternal transmission of the virus. Microspheres synthesized from poly (D, L-lactide-co-glycolide) (PLGA) or poly (D, L-lactide) (PLA) polymers have been considered as promising tools for in vivo delivery of antigens and drugs. Here we describe PLA microspheres synthesized by premix membrane emulsification method and their application in formulating a new microsphere based HB vaccine. To evaluate the immunogenicity of this microsphere vaccine, BALB/c mice were immunized with microsphere vaccine and a series of immunological assays were conducted. Results of Enzyme-linked ImmunoSpot (ELISPOT) assays revealed that the number of interferon-gamma (IFN-gamma)-producing splenocytes and CD8(+) T cells increased significantly in the microsphere vaccine group. Microsphere vaccine group showed enhanced specific cell lysis when compared with HB surface antigen (HBsAg) only group in Cr-51 cytotoxicity assays. Moreover, microsphere vaccine elicited a comparable level of antibody production as that of HB vaccine administered with alum adjuvant. We show that phagocytosis of HBsAg by dendritic cells is more pronounced in microsphere vaccine group when compared with other control groups. These results clearly demonstrate the potential of using PLA microspheres as effective HB vaccine adjuvants for an enhanced Th1 immune response.; Hepatitis B (HB) infection caused by Hepatitis B virus (HBV) is the most common liver disease in the world. HB vaccine, when administered in conjunction with alum adjuvants, induces Th2 immunity that confers protection against HBV. However, currently available vaccine formulations and adjuvants do not elicit adequate Th1 and CTL responses that are important for prevention of maternal transmission of the virus. Microspheres synthesized from poly (D, L-lactide-co-glycolide) (PLGA) or poly (D, L-lactide) (PLA) polymers have been considered as promising tools for in vivo delivery of antigens and drugs. Here we describe PLA microspheres synthesized by premix membrane emulsification method and their application in formulating a new microsphere based HB vaccine. To evaluate the immunogenicity of this microsphere vaccine, BALB/c mice were immunized with microsphere vaccine and a series of immunological assays were conducted. Results of Enzyme-linked ImmunoSpot (ELISPOT) assays revealed that the number of interferon-gamma (IFN-gamma)-producing splenocytes and CD8(+) T cells increased significantly in the microsphere vaccine group. Microsphere vaccine group showed enhanced specific cell lysis when compared with HB surface antigen (HBsAg) only group in Cr-51 cytotoxicity assays. Moreover, microsphere vaccine elicited a comparable level of antibody production as that of HB vaccine administered with alum adjuvant. We show that phagocytosis of HBsAg by dendritic cells is more pronounced in microsphere vaccine group when compared with other control groups. These results clearly demonstrate the potential of using PLA microspheres as effective HB vaccine adjuvants for an enhanced Th1 immune response.
KeywordPolylactide Microspheres Hbsag Ifn-gamma Ctl Anti-hbs
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.4161/hv.29559
URL查看原文
Indexed BySCI
Language英语
WOS KeywordVIRUS ; MICROPARTICLES ; RELEASE ; VACCINE ; IMMUNOGENICITY ; NANOPARTICLES ; IMMUNIZATION ; PATHOGENESIS ; INDUCTION ; INFECTION
WOS Research AreaBiotechnology & Applied Microbiology ; Immunology
WOS SubjectBiotechnology & Applied Microbiology ; Immunology
WOS IDWOS:000344318300034
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/11684
Collection研究所(批量导入)
Affiliation1.Minist Hlth Res Qual & Standardizat Biotech Prod, Key Lab, Natl Inst Food & Drug Control, Div Hepatitis Virus Vaccine, Beijing, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing, Peoples R China
3.Hualan Biol Engn Inc Xinxiang, Xinxiang, Henan, Peoples R China
Recommended Citation
GB/T 7714
Qiu, Shaohui,Wei, Qiang,Liang, Zhenglun,et al. Biodegradable polylactide microspheres enhance specific immune response induced by Hepatitis B surface antigen[J]. HUMAN VACCINES & IMMUNOTHERAPEUTICS,2014,10(8):2350-2356.
APA Qiu, Shaohui.,Wei, Qiang.,Liang, Zhenglun.,Ma, Guanghui.,Wang, Lianyan.,...&Hu, Zhongyu.(2014).Biodegradable polylactide microspheres enhance specific immune response induced by Hepatitis B surface antigen.HUMAN VACCINES & IMMUNOTHERAPEUTICS,10(8),2350-2356.
MLA Qiu, Shaohui,et al."Biodegradable polylactide microspheres enhance specific immune response induced by Hepatitis B surface antigen".HUMAN VACCINES & IMMUNOTHERAPEUTICS 10.8(2014):2350-2356.
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