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SERS Imaging of Cell-Surface Biomolecules Metabolically Labeled with Bioorthogonal Raman Reporters
Alternative TitleChem.-Asian J.
Xiao, Ming1; Lin, Liang1; Li, Zefan1; Liu, Jie2; Hong, Senlian1; Li, Yaya1; Zheng, Meiling2; Duan, Xuanming2; Chen, Xing1
2014-08-01
Source PublicationCHEMISTRY-AN ASIAN JOURNAL
ISSN1861-4728
Volume9Issue:8Pages:2040-2044
AbstractLive imaging of biomolecules with high specificity and sensitivity as well as minimal perturbation is essential for studying cellular processes. Here, we report the development of a bioorthogonal surface-enhanced Raman scattering (SERS) imaging approach that exploits small Raman reporters for visualizing cell-surface biomolecules. The cells were cultured and imaged by SERS microscopy on arrays of Raman-enhancing nanoparticles coated on silicon wafers or glass slides. The Raman reporters including azides, alkynes, and carbon-deuterium bonds are small in size and spectroscopically bioorthogonal (background-free). We demonstrated that various cell-surface biomolecules including proteins, glycans, and lipids were metabolically incorporated with the corresponding precursors bearing a Raman reporter and visualized by SERS microscopy. The coupling of SERS microscopy with bioorthogonal Raman reporters expands the capabilities of live-cell microscopy beyond the modalities of fluorescence and label-free imaging.; Live imaging of biomolecules with high specificity and sensitivity as well as minimal perturbation is essential for studying cellular processes. Here, we report the development of a bioorthogonal surface-enhanced Raman scattering (SERS) imaging approach that exploits small Raman reporters for visualizing cell-surface biomolecules. The cells were cultured and imaged by SERS microscopy on arrays of Raman-enhancing nanoparticles coated on silicon wafers or glass slides. The Raman reporters including azides, alkynes, and carbon-deuterium bonds are small in size and spectroscopically bioorthogonal (background-free). We demonstrated that various cell-surface biomolecules including proteins, glycans, and lipids were metabolically incorporated with the corresponding precursors bearing a Raman reporter and visualized by SERS microscopy. The coupling of SERS microscopy with bioorthogonal Raman reporters expands the capabilities of live-cell microscopy beyond the modalities of fluorescence and label-free imaging.
KeywordBiomolecules Bioorthogonality Cell Surface Raman Imaging Sers Imaging
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1002/asia.201402151
URL查看原文
Indexed BySCI
Language英语
WOS KeywordSELF-ASSEMBLED MONOLAYERS ; NONCANONICAL AMINO-ACIDS ; IN-VIVO ; FLUORESCENCE MICROSCOPY ; SCATTERING ; PHOSPHOLIPIDS ; NANOPARTICLES ; SPECTROSCOPY ; CHEMISTRY ; DISCOVERY
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000342675900007
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/11690
Collection研究所(批量导入)
Affiliation1.Peking Univ, Peking Tsinghua Ctr Life Sci, Synthet & Funct Biomol Ctr, Coll Chem & Mol Engn,Dept Chem Biol,Beijing Natl, Beijing 100871, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Xiao, Ming,Lin, Liang,Li, Zefan,et al. SERS Imaging of Cell-Surface Biomolecules Metabolically Labeled with Bioorthogonal Raman Reporters[J]. CHEMISTRY-AN ASIAN JOURNAL,2014,9(8):2040-2044.
APA Xiao, Ming.,Lin, Liang.,Li, Zefan.,Liu, Jie.,Hong, Senlian.,...&Chen, Xing.(2014).SERS Imaging of Cell-Surface Biomolecules Metabolically Labeled with Bioorthogonal Raman Reporters.CHEMISTRY-AN ASIAN JOURNAL,9(8),2040-2044.
MLA Xiao, Ming,et al."SERS Imaging of Cell-Surface Biomolecules Metabolically Labeled with Bioorthogonal Raman Reporters".CHEMISTRY-AN ASIAN JOURNAL 9.8(2014):2040-2044.
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