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Formation of Nitrile Hydratase Cross-Linked Enzyme Aggregates in Mesoporous Onion-like Silica: Preparation and Catalytic Properties
Alternative TitleInd. Eng. Chem. Res.
Gao, J.; Wang, Q.; Jiang, Y. J.; Gao, J. K.; Liu, Z. H.; Zhou, L. Y.; Zhang, Y. F.
2015
Source PublicationIndustrial & Engineering Chemistry Research
Issue1Pages:83-90
Abstract

Nitrile hydratase cross-linked enzyme aggregates (CLEAs) were formed in mesoporous onion-like silica (NHase-CLEAs@MOS) by using macromolecular dextran polyaldehyde as a cross-linker through the carrier-bound CLEAs method. The effect of preparation parameters on the recovery of enzyme activity was investigated. The properties such as pH, thermal and storage stability, and kinetic parameters of NHase-CLEAs@MOS were also studied. The maximum amount of NHase absorbed in MOS was 535 mg/g. Under optimized conditions, the maximum activity recovery of NHase-CLEAs@MOS was 48.2%. The stabilities of NHase-CLEAs@MOS were improved significantly compared to the NHase@MOS prepared by physical adsorption and free NHase. This work demonstrated that the mesoporous onion-like silica can be efficiently employed as host materials for NHase immobilization, and the carrier-bound CLEAs method can lead to enhanced activity and stability of the immobilized enzymes.

KeywordAlpha-amylase Immobilization Cleas Stabilization Biocatalysis Laccase Decolorization Bioconversion Acrylamide Linking
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Indexed BySCI
Language英语
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/11731
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
Gao, J.,Wang, Q.,Jiang, Y. J.,et al. Formation of Nitrile Hydratase Cross-Linked Enzyme Aggregates in Mesoporous Onion-like Silica: Preparation and Catalytic Properties[J]. Industrial & Engineering Chemistry Research,2015(1):83-90.
APA Gao, J..,Wang, Q..,Jiang, Y. J..,Gao, J. K..,Liu, Z. H..,...&Zhang, Y. F..(2015).Formation of Nitrile Hydratase Cross-Linked Enzyme Aggregates in Mesoporous Onion-like Silica: Preparation and Catalytic Properties.Industrial & Engineering Chemistry Research(1),83-90.
MLA Gao, J.,et al."Formation of Nitrile Hydratase Cross-Linked Enzyme Aggregates in Mesoporous Onion-like Silica: Preparation and Catalytic Properties".Industrial & Engineering Chemistry Research .1(2015):83-90.
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