Formation of Nitrile Hydratase Cross-Linked Enzyme Aggregates in Mesoporous Onion-like Silica: Preparation and Catalytic Properties

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	Formation of Nitrile Hydratase Cross-Linked Enzyme Aggregates in Mesoporous Onion-like Silica: Preparation and Catalytic Properties
Alternative Title	Ind. Eng. Chem. Res.
	Gao, J.; Wang, Q.; Jiang, Y. J.; Gao, J. K.; Liu, Z. H.; Zhou, L. Y.; Zhang, Y. F.
	2015
Source Publication	Industrial & Engineering Chemistry Research
Issue	1 Pages:83-90
Abstract	Nitrile hydratase cross-linked enzyme aggregates (CLEAs) were formed in mesoporous onion-like silica (NHase-CLEAs@MOS) by using macromolecular dextran polyaldehyde as a cross-linker through the carrier-bound CLEAs method. The effect of preparation parameters on the recovery of enzyme activity was investigated. The properties such as pH, thermal and storage stability, and kinetic parameters of NHase-CLEAs@MOS were also studied. The maximum amount of NHase absorbed in MOS was 535 mg/g. Under optimized conditions, the maximum activity recovery of NHase-CLEAs@MOS was 48.2%. The stabilities of NHase-CLEAs@MOS were improved significantly compared to the NHase@MOS prepared by physical adsorption and free NHase. This work demonstrated that the mesoporous onion-like silica can be efficiently employed as host materials for NHase immobilization, and the carrier-bound CLEAs method can lead to enhanced activity and stability of the immobilized enzymes.
Keyword	Alpha-amylase Immobilization Cleas Stabilization Biocatalysis Laccase Decolorization Bioconversion Acrylamide Linking
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Indexed By	SCI
Language	英语
Document Type	期刊论文
Identifier	http://ir.ipe.ac.cn/handle/122111/11731
Collection	研究所（批量导入）
Recommended Citation GB/T 7714	Gao, J.,Wang, Q.,Jiang, Y. J.,et al. Formation of Nitrile Hydratase Cross-Linked Enzyme Aggregates in Mesoporous Onion-like Silica: Preparation and Catalytic Properties[J]. Industrial & Engineering Chemistry Research,2015(1):83-90.
APA	Gao, J..,Wang, Q..,Jiang, Y. J..,Gao, J. K..,Liu, Z. H..,...&Zhang, Y. F..(2015).Formation of Nitrile Hydratase Cross-Linked Enzyme Aggregates in Mesoporous Onion-like Silica: Preparation and Catalytic Properties.Industrial & Engineering Chemistry Research(1),83-90.
MLA	Gao, J.,et al."Formation of Nitrile Hydratase Cross-Linked Enzyme Aggregates in Mesoporous Onion-like Silica: Preparation and Catalytic Properties".Industrial & Engineering Chemistry Research .1(2015):83-90.

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