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Polyelectrolyte Doped Hollow Nanofibers for Positional Assembly of Bienzyme System for Cascade Reaction at O/W Interface
Alternative TitleACS Catal.
Ji, Xiaoyuan1,2; Su, Zhiguo1,5; Wang, Ping1,3,4; Ma, Guanghui1; Zhang, Songping1,5
2014-12-01
Source PublicationACS CATALYSIS
ISSN2155-5435
Volume4Issue:12Pages:4548-4559
AbstractCationic polyelectrolyte doped hollow nanofibers prepared via facial coaxial electrospinning technology have been used for positional assembly of two enzymes, glucose oxidase (GOD) and Candida antactica lipase B (CALB), at two different positions, namely, in their lumen and on their surface. Therefore, the result is four combinations, including lumen (GOD+CALB), surface (GOD+CALB), surface (GOD)-lumen (CALB), and lumen (GOD)-surface (CALB). Surface attachment of enzymes was achieved by layer-by-layer (LbL) technology, which is based on the ion-exchange interactions between oppositely charged enzymes and polyelectrolyte that was doped in hollow nanofibers; whereas placing enzymes inside the lumen of hollow nanofibers was realized by in situ encapsulation during coelectrospinning. The hollow nanofibers-based biencyine systems were used for a cascade reaction in an oil-aqueous biphasic system, in which glucose was oxidized by GOD to generate H2O2, which was used as substrate and oxidant for CALB-catalyzed epoxidation of oleic acid in the second step. The bienzyme nanofibers membrane was found to float spontaneously at the O/W interface, which is advantageous to biphasic biocatalysis. Assembly strategies of the two enzymes affect their biocatalytic efficiency significantly by influencing the utilization efficiency of H2O2 in the reaction process. The highest reaction rate was attained by lumen (GOD)-surface (CALB), corresponding to 114.45 times enhancement as compared to that of the free bienzyme system.; Cationic polyelectrolyte doped hollow nanofibers prepared via facial coaxial electrospinning technology have been used for positional assembly of two enzymes, glucose oxidase (GOD) and Candida antactica lipase B (CALB), at two different positions, namely, in their lumen and on their surface. Therefore, the result is four combinations, including lumen (GOD+CALB), surface (GOD+CALB), surface (GOD)-lumen (CALB), and lumen (GOD)-surface (CALB). Surface attachment of enzymes was achieved by layer-by-layer (LbL) technology, which is based on the ion-exchange interactions between oppositely charged enzymes and polyelectrolyte that was doped in hollow nanofibers; whereas placing enzymes inside the lumen of hollow nanofibers was realized by in situ encapsulation during coelectrospinning. The hollow nanofibers-based biencyine systems were used for a cascade reaction in an oil-aqueous biphasic system, in which glucose was oxidized by GOD to generate H2O2, which was used as substrate and oxidant for CALB-catalyzed epoxidation of oleic acid in the second step. The bienzyme nanofibers membrane was found to float spontaneously at the O/W interface, which is advantageous to biphasic biocatalysis. Assembly strategies of the two enzymes affect their biocatalytic efficiency significantly by influencing the utilization efficiency of H2O2 in the reaction process. The highest reaction rate was attained by lumen (GOD)-surface (CALB), corresponding to 114.45 times enhancement as compared to that of the free bienzyme system.
KeywordCascade Reactions Coaxial Electrospinning Polyelectrolyte Hollow Nanofibers Positional Assembly
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1021/cs501383j
URL查看原文
Indexed BySCI
Language英语
WOS KeywordUNSATURATED FATTY-ACID ; CHEMOENZYMATIC EPOXIDATION ; BINDING CHLOROPEROXIDASE ; ENZYME IMMOBILIZATION ; ARTIFICIAL CELLS ; BRADFORD METHOD ; SOYBEAN OIL ; PLANT OILS ; BIOCATALYSIS ; PERFORMANCE
WOS Research AreaChemistry
WOS SubjectChemistry, Physical
WOS IDWOS:000346041600035
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/11754
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA
4.Univ Minnesota, Inst Biotechnol, St Paul, MN 55108 USA
5.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300072, Peoples R China
Recommended Citation
GB/T 7714
Ji, Xiaoyuan,Su, Zhiguo,Wang, Ping,et al. Polyelectrolyte Doped Hollow Nanofibers for Positional Assembly of Bienzyme System for Cascade Reaction at O/W Interface[J]. ACS CATALYSIS,2014,4(12):4548-4559.
APA Ji, Xiaoyuan,Su, Zhiguo,Wang, Ping,Ma, Guanghui,&Zhang, Songping.(2014).Polyelectrolyte Doped Hollow Nanofibers for Positional Assembly of Bienzyme System for Cascade Reaction at O/W Interface.ACS CATALYSIS,4(12),4548-4559.
MLA Ji, Xiaoyuan,et al."Polyelectrolyte Doped Hollow Nanofibers for Positional Assembly of Bienzyme System for Cascade Reaction at O/W Interface".ACS CATALYSIS 4.12(2014):4548-4559.
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