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Facile immobilization of enzyme on three dimensionally ordered macroporous silica via a biomimetic coating
Alternative TitleNew J. Chem.
Jiang, Yanjun1; Wang, Yaping1; Wang, Hua1; Zhou, Liya1; Gao, Jing1; Zhang, Yufei2; Zhang, Xu1; Wang, Xiaomei1; Li, Jian3
2015
Source PublicationNEW JOURNAL OF CHEMISTRY
ISSN1144-0546
Volume39Issue:2Pages:978-984
Abstract

This study describes a general strategy using norepinephrine (NE) as a stable anchor for the immobilization of enzyme onto three-dimensionally ordered macroporous (3DOM) silica. Penicillin G acylase (PGA) was chosen as a model enzyme. The effect of pH and temperature on the activity of PGA@PN-SiO2 (PGA immobilized on poly(norepinephrine)-modified 3DOM silica) was investigated. The operational stability, storage stability and kinetic properties of the PGA@PN-SiO2 were also examined. Compared with free PGA and PGA@SiO2 (PGA immobilized on blank 3DOM silica through physical adsorption), the stabilities of PGA@PN-SiO2 were improved significantly. PGA@PN-SiO2 retained high activity during the hydrolysis in a continuous packed-bed reactor (PBR) after 30 catalytic cycles, which demonstrated that PGA@PN-SiO2 can bear endurance of continuous catalysis. The method presented in this study has broad potential for immobilizing enzymes and other biomolecules.

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This study describes a general strategy using norepinephrine (NE) as a stable anchor for the immobilization of enzyme onto three-dimensionally ordered macroporous (3DOM) silica. Penicillin G acylase (PGA) was chosen as a model enzyme. The effect of pH and temperature on the activity of PGA@PN-SiO2 (PGA immobilized on poly(norepinephrine)-modified 3DOM silica) was investigated. The operational stability, storage stability and kinetic properties of the PGA@PN-SiO2 were also examined. Compared with free PGA and PGA@SiO2 (PGA immobilized on blank 3DOM silica through physical adsorption), the stabilities of PGA@PN-SiO2 were improved significantly. PGA@PN-SiO2 retained high activity during the hydrolysis in a continuous packed-bed reactor (PBR) after 30 catalytic cycles, which demonstrated that PGA@PN-SiO2 can bear endurance of continuous catalysis. The method presented in this study has broad potential for immobilizing enzymes and other biomolecules.

KeywordPenicillin-g Acylase Mesostructured Cellular Foams Mesoporous Silica Cross-linking Biocatalysts Improvement Stability Lipase
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1039/c4nj01947d
URL查看原文
Indexed BySCI
Language英语
WOS KeywordPENICILLIN-G ACYLASE ; MESOSTRUCTURED CELLULAR FOAMS ; MESOPOROUS SILICA ; CROSS-LINKING ; BIOCATALYSTS ; IMPROVEMENT ; STABILITY ; LIPASE
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000349222800026
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/11761
Collection研究所(批量导入)
Affiliation1.Hebei Univ Technol, Sch Chem Engn & Technol, Tianjin 300130, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
3.Tianjin Univ Sci & Technolgy, Coll Mat Sci & Chem Engn, Tianjin 300457, Peoples R China
Recommended Citation
GB/T 7714
Jiang, Yanjun,Wang, Yaping,Wang, Hua,et al. Facile immobilization of enzyme on three dimensionally ordered macroporous silica via a biomimetic coating[J]. NEW JOURNAL OF CHEMISTRY,2015,39(2):978-984.
APA Jiang, Yanjun.,Wang, Yaping.,Wang, Hua.,Zhou, Liya.,Gao, Jing.,...&Li, Jian.(2015).Facile immobilization of enzyme on three dimensionally ordered macroporous silica via a biomimetic coating.NEW JOURNAL OF CHEMISTRY,39(2),978-984.
MLA Jiang, Yanjun,et al."Facile immobilization of enzyme on three dimensionally ordered macroporous silica via a biomimetic coating".NEW JOURNAL OF CHEMISTRY 39.2(2015):978-984.
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