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Preparation of large-sized highly uniform agarose beads by novel rotating membrane emulsification
Alternative TitleJ. Membr. Sci.
Li, Xiao-Qing1,2; Li, Qiang1; Gong, Fang-Ling1; Lei, Jian-Du3; Zhao, Xi1,2; Ma, Guang-Hui1; Su, Zhi-Guo1
2015-02-15
Source PublicationJOURNAL OF MEMBRANE SCIENCE
ISSN0376-7388
Volume476Issue:FEBPages:30-39
Abstract

A novel rotating membrane emulsification setup incorporating a 100 mu m pore diameter stainless steel hydrophobic membrane is used to produce MO emulsions consisting of 4 wt% hexaglycerin penta ester (PO-500) as emulsifier, the mixture of liquid paraffin (LP) and petroleum ether (PE) in 11:1 (v/v) as continuous oil phase and agarose solution as the dispersed phase. The agarose beads of average sizes between 108.4 and 385.4 mu m can be obtained after the emulsion solidification process. The membrane rotational speed (500-2000 rpm), agarose concentration (2-4 wt%) and oil phase composition are all investigated as to their effect on emulsion droplet size and size uniformity of agarose beads. The optimal conditions for producing uniform agarose beads are an agarose solution of 3 wt%, a mixture of liquid paraffin and petroleum ether in 11:1 (v/v) and a membrane rotational speed of 900 rpm, under which the average bead diameter is 220 pm with a very narrow size distribution value of 0.76 (Span value). A model describing the operation is presented to predict droplet size under a certain condition and the prediction results fit with the experimental data fairly well. The emulsification reproducibility and stable beads properties demonstrate that the rotating membrane emulsification is a manufacturing protocol for uniform droplets and beads of controlled size. (C) 2014 Elsevier BY. All rights reserved.

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A novel rotating membrane emulsification setup incorporating a 100 mu m pore diameter stainless steel hydrophobic membrane is used to produce MO emulsions consisting of 4 wt% hexaglycerin penta ester (PO-500) as emulsifier, the mixture of liquid paraffin (LP) and petroleum ether (PE) in 11:1 (v/v) as continuous oil phase and agarose solution as the dispersed phase. The agarose beads of average sizes between 108.4 and 385.4 mu m can be obtained after the emulsion solidification process. The membrane rotational speed (500-2000 rpm), agarose concentration (2-4 wt%) and oil phase composition are all investigated as to their effect on emulsion droplet size and size uniformity of agarose beads. The optimal conditions for producing uniform agarose beads are an agarose solution of 3 wt%, a mixture of liquid paraffin and petroleum ether in 11:1 (v/v) and a membrane rotational speed of 900 rpm, under which the average bead diameter is 220 pm with a very narrow size distribution value of 0.76 (Span value). A model describing the operation is presented to predict droplet size under a certain condition and the prediction results fit with the experimental data fairly well. The emulsification reproducibility and stable beads properties demonstrate that the rotating membrane emulsification is a manufacturing protocol for uniform droplets and beads of controlled size. (C) 2014 Elsevier BY. All rights reserved.

KeywordRotating Membrane Emulsification Agarose Beads Droplet Formation Model Column Efficiency
SubtypeArticle
WOS HeadingsScience & Technology ; Technology ; Physical Sciences
DOI10.1016/j.memsci.2014.11.017
URL查看原文
Indexed BySCI
Language英语
WOS KeywordCROSS-FLOW MEMBRANE ; EMULSIONS ; MANUFACTURE ; PORE
WOS Research AreaEngineering ; Polymer Science
WOS SubjectEngineering, Chemical ; Polymer Science
WOS IDWOS:000349382600004
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/11778
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Beijing Forestry Univ, Beijing Key Lab Lignocellulos Chem, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Li, Xiao-Qing,Li, Qiang,Gong, Fang-Ling,et al. Preparation of large-sized highly uniform agarose beads by novel rotating membrane emulsification[J]. JOURNAL OF MEMBRANE SCIENCE,2015,476(FEB):30-39.
APA Li, Xiao-Qing.,Li, Qiang.,Gong, Fang-Ling.,Lei, Jian-Du.,Zhao, Xi.,...&Su, Zhi-Guo.(2015).Preparation of large-sized highly uniform agarose beads by novel rotating membrane emulsification.JOURNAL OF MEMBRANE SCIENCE,476(FEB),30-39.
MLA Li, Xiao-Qing,et al."Preparation of large-sized highly uniform agarose beads by novel rotating membrane emulsification".JOURNAL OF MEMBRANE SCIENCE 476.FEB(2015):30-39.
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