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Novel Magnetic Cross-Linked Lipase Aggregates for Improving the Resolution of (R, S)-2-octanol
Alternative TitleChirality
Liu, Ying; Guo, Chen; Liu, Chun-Zhao1
2015-03-01
Source PublicationCHIRALITY
ISSN0899-0042
Volume27Issue:3Pages:199-204
Abstract

Novel magnetic cross-linked lipase aggregates were fabricated by immobilizing the cross-linked lipase aggregates onto magnetic particles with a high number of -NH2 terminal groups using p-benzoquinone as the cross-linking agent. At the optimal fabrication conditions, 100% of immobilization efficiency and 139% of activity recovery of the magnetic cross-linked lipase aggregates were achieved. The magnetic cross-linked lipase aggregates were able to efficiently resolve (R, S)-2-octanol, and retained 100% activity and 100% enantioselectivity after 10cycles of reuse, whereas the cross-linked lipase aggregates only retained about 50% activity and 70% enantioselectivity due to insufficient cross-linking. These results provide a great potential for industrial applications of the magnetic cross-linked lipase aggregates. Chirality 27:199-204, 2015. (c) 2014 Wiley Periodicals, Inc.

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Novel magnetic cross-linked lipase aggregates were fabricated by immobilizing the cross-linked lipase aggregates onto magnetic particles with a high number of -NH2 terminal groups using p-benzoquinone as the cross-linking agent. At the optimal fabrication conditions, 100% of immobilization efficiency and 139% of activity recovery of the magnetic cross-linked lipase aggregates were achieved. The magnetic cross-linked lipase aggregates were able to efficiently resolve (R, S)-2-octanol, and retained 100% activity and 100% enantioselectivity after 10cycles of reuse, whereas the cross-linked lipase aggregates only retained about 50% activity and 70% enantioselectivity due to insufficient cross-linking. These results provide a great potential for industrial applications of the magnetic cross-linked lipase aggregates. Chirality 27:199-204, 2015. (c) 2014 Wiley Periodicals, Inc.

KeywordYarrowia Lipolytica Lipase Magnetic Nanoparticles Cross-linked Enzyme Aggregates Immobilization (r s)-2-octanol Enantioselectivity
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1002/chir.22411
URL查看原文
Indexed BySCI
Language英语
WOS KeywordOPTIMUM PERFORMANCE ; CLEAS ; IMMOBILIZATION ; BIOCATALYSTS
WOS Research AreaPharmacology & Pharmacy ; Chemistry
WOS SubjectChemistry, Medicinal ; Chemistry, Analytical ; Chemistry, Organic ; Pharmacology & Pharmacy
WOS IDWOS:000350137200003
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/11798
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Liu, Ying,Guo, Chen,Liu, Chun-Zhao. Novel Magnetic Cross-Linked Lipase Aggregates for Improving the Resolution of (R, S)-2-octanol[J]. CHIRALITY,2015,27(3):199-204.
APA Liu, Ying,Guo, Chen,&Liu, Chun-Zhao.(2015).Novel Magnetic Cross-Linked Lipase Aggregates for Improving the Resolution of (R, S)-2-octanol.CHIRALITY,27(3),199-204.
MLA Liu, Ying,et al."Novel Magnetic Cross-Linked Lipase Aggregates for Improving the Resolution of (R, S)-2-octanol".CHIRALITY 27.3(2015):199-204.
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