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Uniform-sized water-in-oil vaccine formulations enhance immune response against Newcastle disease and avian influenza in chickens
Alternative TitleInt. Immunopharmacol.
Liu, Yuan1,2; Wang, Lianyan1; Zhang, Yu3; Zhang, Weifeng1,2; Chen, Xiaoming1,2; Yang, Tingyuan1; Wang, Zhuo3; Ma, Guanghui1
2014-12-01
Source PublicationINTERNATIONAL IMMUNOPHARMACOLOGY
ISSN1567-5769
Volume23Issue:2Pages:603-608
AbstractWater-in-oil (W/O) emulsion is one of the best "one-shot" delivery system for antigen to generate high and durable antibody response. Here a facile method using premixed Shirasu Porous Glass (SPG) membrane emulsification technique was employed to fabricate uniform-sized emulsion and mechanical stirring was employed to prepare non-uniform-sized emulsion as control. In release kinetics studies, uniform-sized emulsion showed low initial release, and during the followed 17 days, uniform-sized emulsions released antigen faster than emulsions of non-uniform size. In addition, vaccination studies against Newcastle disease virus (NDV) and avian Influenza virus (AIV) demonstrated that emulsion of uniform size induced higher HI antibodies and antigen-specific IgG titers. Furthermore, chickens vaccinated with emulsion of uniform size had a significantly greater ratio of CD8(+)T cells to CD4(+)T cells and a higher percentage of CD8(+)CD4(+)T cells. Taken together, these results indicated that emulsion of uniform size induced a more potent immune response than emulsion prepared by mechanical stirring, and warranted the use of SPG membrane emulsification technique in generating new avian vaccines. (C) 2014 Elsevier B.V. All rights reserved.; Water-in-oil (W/O) emulsion is one of the best "one-shot" delivery system for antigen to generate high and durable antibody response. Here a facile method using premixed Shirasu Porous Glass (SPG) membrane emulsification technique was employed to fabricate uniform-sized emulsion and mechanical stirring was employed to prepare non-uniform-sized emulsion as control. In release kinetics studies, uniform-sized emulsion showed low initial release, and during the followed 17 days, uniform-sized emulsions released antigen faster than emulsions of non-uniform size. In addition, vaccination studies against Newcastle disease virus (NDV) and avian Influenza virus (AIV) demonstrated that emulsion of uniform size induced higher HI antibodies and antigen-specific IgG titers. Furthermore, chickens vaccinated with emulsion of uniform size had a significantly greater ratio of CD8(+)T cells to CD4(+)T cells and a higher percentage of CD8(+)CD4(+)T cells. Taken together, these results indicated that emulsion of uniform size induced a more potent immune response than emulsion prepared by mechanical stirring, and warranted the use of SPG membrane emulsification technique in generating new avian vaccines. (C) 2014 Elsevier B.V. All rights reserved.
KeywordEmulsion Immune Response Membrane Emulsification Size Distribution Vaccine
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.intimp.2014.10.011
URL查看原文
Indexed BySCI
Language英语
WOS KeywordADJUVANT ACTIVITY ; T-CELLS ; RELEASE ; EFFICACY ; POLYSACCHARIDES ; NANOPARTICLES ; PARTICLES ; LIPOSOME ; KINETICS ; IMPROVE
WOS Research AreaImmunology ; Pharmacology & Pharmacy
WOS SubjectImmunology ; Pharmacology & Pharmacy
WOS IDWOS:000346954000032
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Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/11799
Collection研究所(批量导入)
Affiliation1.Chinese Acad ofSciences, Inst Proc Engn, PIA Key Lab Biopharmaceut Prod & Formulat Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Beijing Sinder Weite Technol Co Ltd, Beijing 101302, Peoples R China
Recommended Citation
GB/T 7714
Liu, Yuan,Wang, Lianyan,Zhang, Yu,et al. Uniform-sized water-in-oil vaccine formulations enhance immune response against Newcastle disease and avian influenza in chickens[J]. INTERNATIONAL IMMUNOPHARMACOLOGY,2014,23(2):603-608.
APA Liu, Yuan.,Wang, Lianyan.,Zhang, Yu.,Zhang, Weifeng.,Chen, Xiaoming.,...&Ma, Guanghui.(2014).Uniform-sized water-in-oil vaccine formulations enhance immune response against Newcastle disease and avian influenza in chickens.INTERNATIONAL IMMUNOPHARMACOLOGY,23(2),603-608.
MLA Liu, Yuan,et al."Uniform-sized water-in-oil vaccine formulations enhance immune response against Newcastle disease and avian influenza in chickens".INTERNATIONAL IMMUNOPHARMACOLOGY 23.2(2014):603-608.
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