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Surface charge of PLA microparticles in regulation of antigen loading, macrophage phagocytosis and activation, and immune effects in vitro
Alternative TitleParticuology
Liu, Yuying1,2; Chen, Xiaoming1,3; Wang, Lianyan1; Yang, Tingyuan1; Yuan, Qipeng2; Ma, Guanghui1
2014-12-01
Source PublicationPARTICUOLOGY
ISSN1674-2001
Volume17Issue:DECPages:74-80
AbstractThe use of microparticles (MPs) as adjuvants has attracted increasing interest in vaccine delivery systems. Many physiochemical characteristics of MPs including hydrodynamic size, surface properties, and morphology can regulate the immune response. Surface charge is an important characteristic of MPs, but how it affects their adjuvanticity remains unknown. In this study, we prepared uniform-sized polylactide MPs coated with various polymers of different positive charge, and investigated how the surface charge affected antigen loading and macrophage phagocytosis and activation in vitro. A higher surface charge greatly enhanced antigen loading and antigen internalization into macrophages, promoted the expression of MHC II and CD80, and increased the secretion level of TNF-alpha. Taken together, these results indicated that surface charge was an important parameter for improving the adjuvanticity of MPs. (C) 2014 Published by Elsevier B.V. on behalf of Chinese Society of Particuology and Institute of Process Engineering, Chinese Academy of Sciences.; The use of microparticles (MPs) as adjuvants has attracted increasing interest in vaccine delivery systems. Many physiochemical characteristics of MPs including hydrodynamic size, surface properties, and morphology can regulate the immune response. Surface charge is an important characteristic of MPs, but how it affects their adjuvanticity remains unknown. In this study, we prepared uniform-sized polylactide MPs coated with various polymers of different positive charge, and investigated how the surface charge affected antigen loading and macrophage phagocytosis and activation in vitro. A higher surface charge greatly enhanced antigen loading and antigen internalization into macrophages, promoted the expression of MHC II and CD80, and increased the secretion level of TNF-alpha. Taken together, these results indicated that surface charge was an important parameter for improving the adjuvanticity of MPs. (C) 2014 Published by Elsevier B.V. on behalf of Chinese Society of Particuology and Institute of Process Engineering, Chinese Academy of Sciences.
KeywordMicroparticle (Mp) Macrophage Surface Charge Antigen Loading Phagocytosis Activation
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
DOI10.1016/j.partic.2014.02.006
URL查看原文
Indexed BySCI
Language英语
WOS KeywordCOMPLEMENT ACTIVATION ; PARTICLE-SIZE ; MICROSPHERES ; ADJUVANTS ; RESPONSES
WOS Research AreaEngineering ; Materials Science
WOS SubjectEngineering, Chemical ; Materials Science, Multidisciplinary
WOS IDWOS:000347656700012
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/11801
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Beijing Univ Chem Technol, Coll Life Sci & Technol, State Key Lab Chem Resource Engn, Beijing 100029, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Liu, Yuying,Chen, Xiaoming,Wang, Lianyan,et al. Surface charge of PLA microparticles in regulation of antigen loading, macrophage phagocytosis and activation, and immune effects in vitro[J]. PARTICUOLOGY,2014,17(DEC):74-80.
APA Liu, Yuying,Chen, Xiaoming,Wang, Lianyan,Yang, Tingyuan,Yuan, Qipeng,&Ma, Guanghui.(2014).Surface charge of PLA microparticles in regulation of antigen loading, macrophage phagocytosis and activation, and immune effects in vitro.PARTICUOLOGY,17(DEC),74-80.
MLA Liu, Yuying,et al."Surface charge of PLA microparticles in regulation of antigen loading, macrophage phagocytosis and activation, and immune effects in vitro".PARTICUOLOGY 17.DEC(2014):74-80.
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